Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic developmental stages of Bos taurus
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2023-02-28
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| Subjects: | Bos taurus, Differentially expressed lncRNAs, Long non-coding RNAs, |
| Online Access: | http://hdl.handle.net/10261/309751 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100008758 https://api.elsevier.com/content/abstract/scopus_id/85146058297 |
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dig-inia-es-10261-3097512024-05-14T20:45:10Z Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic developmental stages of Bos taurus Jali, Itishree Vanamamalai, Venkata Krishna Garg, Priyanka Navarrete-López, Paula Gutiérrez-Adán, Alfonso Sharma, Shailesh Council of Science and Technology UP (India) Ministerio de Ciencia e Innovación (España) Jali, Itishree [0000-0002-6383-8674] Vanamamalai, Venkata Krishna [-0002-9388-521X] Garg, Priyanka [0000-0002-9402-8711] Navarrete-López, Paula [0002-7093-2191] Gutiérrez-Adán, Alfonso [0000-0001-9893-9179] Sharma, Shailesh [0000-0001-7765-1635] Bos taurus Differentially expressed lncRNAs Long non-coding RNAs 13 Pág. X-chromosomes inactivation (XCI) is a phenomenon that aims to equalize the dosage of X-linked gene products between XY males and XX females in mammals. XIST gene is the master regulator of X chromosome inactivation during early embryonic developmental stages of Bos taurus. Biological molecule such as lncRNA plays significant role in the control of XCI, by RNA-based regulatory mechanisms and are non-coding regions of the genome. In our study, using in-silico transcriptome data analysis approach, we analysed RNA-seq data of E35, E39 and E43 samples from bovine genital ridges of early embryonic stages, and identified lncRNA transcripts. More than 7 lakh lncRNA transcripts were identified. Further, our study identified DE-lncRNAs and genes between male and female and studied their co-expression. More than four thousand differentially expressed lncRNAs identified. The co-expression and RT-PCR study in the result showed that there exists an association between the XIST and DE-lncRNAs in early embryonic gonads of bovine at E35. In this study, the association between DE-lncRNAs and XIST gene indicates, the potentially important role of DE-lncRNAs during embryo development in bovine. In conclusion, this study shows there exist an interplay between genes and lncRNAs at transcriptome level of bovine during early embryonic days. This research was supported by CSIR, India (Council of Scientific and Industrial Research, India) for PhD fellowships, DBT, India (Department of Biotechnology, India) for project fellowships and by the Spanish Ministry of Science and Innovation-MICINN (PID2021-122507OB-I00 to A. Gutierrez-Adan. Peer reviewed 2023-05-26T09:39:27Z 2023-05-26T09:39:27Z 2023-02-28 artículo http://purl.org/coar/resource_type/c_6501 International Journal of Biological Macromolecules 229: 896–908 (2023) 0141-8130 http://hdl.handle.net/10261/309751 10.1016/j.ijbiomac.2022.12.221 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100008758 36572076 2-s2.0-85146058297 https://api.elsevier.com/content/abstract/scopus_id/85146058297 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//PID2021-122507OB-I00 Departamento de Reproducción Animal Publisher's version Sí open Elsevier |
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Bos taurus Differentially expressed lncRNAs Long non-coding RNAs Bos taurus Differentially expressed lncRNAs Long non-coding RNAs |
| spellingShingle |
Bos taurus Differentially expressed lncRNAs Long non-coding RNAs Bos taurus Differentially expressed lncRNAs Long non-coding RNAs Jali, Itishree Vanamamalai, Venkata Krishna Garg, Priyanka Navarrete-López, Paula Gutiérrez-Adán, Alfonso Sharma, Shailesh Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic developmental stages of Bos taurus |
| description |
13 Pág. |
| author2 |
Council of Science and Technology UP (India) |
| author_facet |
Council of Science and Technology UP (India) Jali, Itishree Vanamamalai, Venkata Krishna Garg, Priyanka Navarrete-López, Paula Gutiérrez-Adán, Alfonso Sharma, Shailesh |
| format |
artículo |
| topic_facet |
Bos taurus Differentially expressed lncRNAs Long non-coding RNAs |
| author |
Jali, Itishree Vanamamalai, Venkata Krishna Garg, Priyanka Navarrete-López, Paula Gutiérrez-Adán, Alfonso Sharma, Shailesh |
| author_sort |
Jali, Itishree |
| title |
Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic developmental stages of Bos taurus |
| title_short |
Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic developmental stages of Bos taurus |
| title_full |
Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic developmental stages of Bos taurus |
| title_fullStr |
Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic developmental stages of Bos taurus |
| title_full_unstemmed |
Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic developmental stages of Bos taurus |
| title_sort |
identification and differential expression of long non-coding rnas and their association with xist gene during early embryonic developmental stages of bos taurus |
| publisher |
Elsevier |
| publishDate |
2023-02-28 |
| url |
http://hdl.handle.net/10261/309751 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100008758 https://api.elsevier.com/content/abstract/scopus_id/85146058297 |
| work_keys_str_mv |
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| _version_ |
1802819736086511616 |