Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages
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| Format: | artículo biblioteca |
| Language: | English |
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Taylor & Francis
2022-12-19
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| Subjects: | RNA sequencing, Anestrous, Enrichment, Hypothalamus, Pars tuberalis, |
| Online Access: | http://hdl.handle.net/10261/296484 http://dx.doi.org/10.13039/501100010067 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85144302644 |
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dig-inia-es-10261-2964842024-05-14T20:39:17Z Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages Lakhssassi, Kenza Ureña, Irene Marín, Belén Sarto, María Pilar Lahoz, Belén Alabart, José Luis Calvo, Jorge Hugo Serrano, Magdalena Ministerio de Educación y Ciencia (España) European Commission Gobierno de Aragón Lakhssassi, Kenza [0000-0002-9793-7494] Ureña, Irene [0000-0001-9724-9977] Marín, Belén [0000-0002-1590-3347] Sarto, María Pilar [0000-0003-1486-3950] Lahoz, Belén [0000-0001-9394-5027] Alabart, J. L. [0000-0001-5015-8103] Calvo, Jorge Hugo [0000-0001-9513-0219] Serrano, Magdalena [0000-0002-1621-3102] RNA sequencing Anestrous Enrichment Hypothalamus Pars tuberalis 14 Pág. INIA For understanding the molecular events underlying the follicular (F) and luteal (L) phases of estrous cycle, and anestrous (A) phase, the pars tuberalis (PT), and hypothalamus (HT) transcriptomes of 21 ewes were studied. In HT, 72 and 3 differential expression genes (DEGs) were found when comparing F vs. A and L vs. A, respectively. In PT, 6 and 4 DEGs were found in F vs. A and L vs. A comparisons, respectively. Enrichment analysis for DEGs between the F and A phases in the HT revealed significant clusters, mainly associated with actin-binding, and cytoskeleton, that are related to neural plasticity modulated by gonadal steroid hormones, as well as with oxytocin signaling. We found that DEGs in PT had higher differences in expression levels than those found in HT. In this sense, the ITLN was highly upregulated in the F and L vs. A phases, being MRPL57 and IRX4 highly downregulated in L vs. A comparison. The DDC gene in PT, related to LH regulation, was upregulated in the F phase. The gene set enrichment analysis (GSEA) revealed multiple pathways related to neurotransmission and neuronal plasticity. Our study reveals new candidate genes involved in the reproductive stages' transitions in seasonal sheep. K. Lakhssassi is supported by doctoral grant from the Ministry of Education and Science of Spain. This study was partially financed by the Ministry of Education and Science of Spain research projects INIA-RTA2013-00041-C02 and INIA-RTA2015-0090-C03, and the European Union Regional Development funds; and the Research Group Funds of the Aragón Government (Grupo SAGAS Ref. A14_20R). Peer reviewed 2023-03-02T12:56:43Z 2023-03-02T12:56:43Z 2022-12-19 artículo http://purl.org/coar/resource_type/c_6501 Animal Biotechnology: (2022) 1049-5398 http://hdl.handle.net/10261/296484 10.1080/10495398.2022.2155174 http://dx.doi.org/10.13039/501100010067 http://dx.doi.org/10.13039/501100000780 36534535 2-s2.0-85144302644 https://api.elsevier.com/content/abstract/scopus_id/85144302644 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//RTA2013-00041-C02-02/ES/Determinación de la base genética de la fertilidad en el ganado ovino mediante herramientas genómicas..2- Ovino de carne/ info:eu-repo/grantAgreement/MEC//RTA2015-0090-C03 Animal biotechnology Publisher's version https://doi.org/10.1080/10495398.2022.2155174 Sí open Taylor & Francis |
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English |
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RNA sequencing Anestrous Enrichment Hypothalamus Pars tuberalis RNA sequencing Anestrous Enrichment Hypothalamus Pars tuberalis |
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RNA sequencing Anestrous Enrichment Hypothalamus Pars tuberalis RNA sequencing Anestrous Enrichment Hypothalamus Pars tuberalis Lakhssassi, Kenza Ureña, Irene Marín, Belén Sarto, María Pilar Lahoz, Belén Alabart, José Luis Calvo, Jorge Hugo Serrano, Magdalena Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages |
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14 Pág.
INIA |
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Ministerio de Educación y Ciencia (España) |
| author_facet |
Ministerio de Educación y Ciencia (España) Lakhssassi, Kenza Ureña, Irene Marín, Belén Sarto, María Pilar Lahoz, Belén Alabart, José Luis Calvo, Jorge Hugo Serrano, Magdalena |
| format |
artículo |
| topic_facet |
RNA sequencing Anestrous Enrichment Hypothalamus Pars tuberalis |
| author |
Lakhssassi, Kenza Ureña, Irene Marín, Belén Sarto, María Pilar Lahoz, Belén Alabart, José Luis Calvo, Jorge Hugo Serrano, Magdalena |
| author_sort |
Lakhssassi, Kenza |
| title |
Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages |
| title_short |
Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages |
| title_full |
Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages |
| title_fullStr |
Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages |
| title_full_unstemmed |
Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages |
| title_sort |
characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages |
| publisher |
Taylor & Francis |
| publishDate |
2022-12-19 |
| url |
http://hdl.handle.net/10261/296484 http://dx.doi.org/10.13039/501100010067 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85144302644 |
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