Haploid origin of cork oak anther embryos detected by enzyme and RAPD gene markers
In vitro-induced cork oak (Quercus suber L.) embryos from anther cultures proved to be of haploid origin both by enzyme and RAPD gene marker analysis. The problem considered was to ascertain if embryo cultures originated either from a single haploid cell, from a microspore, or from multiple haploid cells. Therefore, a heterozygotic gene was searched for in the parent tree. The gene coding for shikimate dehydrogenase (SKDH1) proved to be heterozygous in the parental tree, and subsequently, these allozymes were screened for the embryos induced in anther cultures from the same tree. Only haploid embryos were found, confirming the microspore origin. Different genotypes were not identified inside each anther by isozyme analysis, probably because of selective pressure for one embryo early in development, but both parental SKDH1 alleles were found in the embryos of different anthers. The banding patterns detected by RAPD markers permitted the identification of multiple microspore origins inside each anther.
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Language: | English |
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University of Chicago Press
2000
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Subjects: | Haploid, Cork oak, Quercus suber, RAPD markers, |
Online Access: | http://hdl.handle.net/20.500.12792/4921 http://hdl.handle.net/10261/294816 |
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dig-inia-es-10261-2948162023-02-20T10:42:16Z Haploid origin of cork oak anther embryos detected by enzyme and RAPD gene markers Bueno, M. A. Agúndez, M. D. Gomez, A. Carrascosa, M. J. Manzanera, J. A. Haploid Cork oak Quercus suber RAPD markers In vitro-induced cork oak (Quercus suber L.) embryos from anther cultures proved to be of haploid origin both by enzyme and RAPD gene marker analysis. The problem considered was to ascertain if embryo cultures originated either from a single haploid cell, from a microspore, or from multiple haploid cells. Therefore, a heterozygotic gene was searched for in the parent tree. The gene coding for shikimate dehydrogenase (SKDH1) proved to be heterozygous in the parental tree, and subsequently, these allozymes were screened for the embryos induced in anther cultures from the same tree. Only haploid embryos were found, confirming the microspore origin. Different genotypes were not identified inside each anther by isozyme analysis, probably because of selective pressure for one embryo early in development, but both parental SKDH1 alleles were found in the embryos of different anthers. The banding patterns detected by RAPD markers permitted the identification of multiple microspore origins inside each anther. 2023-02-20T10:42:16Z 2023-02-20T10:42:16Z 2000 journal article International Journal of Plant Sciences 161(3): 363-367 (2000) 1058-5893 http://hdl.handle.net/20.500.12792/4921 http://hdl.handle.net/10261/294816 10.1086/314265 1537-5315 en none University of Chicago Press |
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Haploid Cork oak Quercus suber RAPD markers Haploid Cork oak Quercus suber RAPD markers Bueno, M. A. Agúndez, M. D. Gomez, A. Carrascosa, M. J. Manzanera, J. A. Haploid origin of cork oak anther embryos detected by enzyme and RAPD gene markers |
description |
In vitro-induced cork oak (Quercus suber L.) embryos from anther cultures proved to be of haploid origin both by enzyme and RAPD gene marker analysis. The problem considered was to ascertain if embryo cultures originated either from a single haploid cell, from a microspore, or from multiple haploid cells. Therefore, a heterozygotic gene was searched for in the parent tree. The gene coding for shikimate dehydrogenase (SKDH1) proved to be heterozygous in the parental tree, and subsequently, these allozymes were screened for the embryos induced in anther cultures from the same tree. Only haploid embryos were found, confirming the microspore origin. Different genotypes were not identified inside each anther by isozyme analysis, probably because of selective pressure for one embryo early in development, but both parental SKDH1 alleles were found in the embryos of different anthers. The banding patterns detected by RAPD markers permitted the identification of multiple microspore origins inside each anther. |
format |
journal article |
topic_facet |
Haploid Cork oak Quercus suber RAPD markers |
author |
Bueno, M. A. Agúndez, M. D. Gomez, A. Carrascosa, M. J. Manzanera, J. A. |
author_facet |
Bueno, M. A. Agúndez, M. D. Gomez, A. Carrascosa, M. J. Manzanera, J. A. |
author_sort |
Bueno, M. A. |
title |
Haploid origin of cork oak anther embryos detected by enzyme and RAPD gene markers |
title_short |
Haploid origin of cork oak anther embryos detected by enzyme and RAPD gene markers |
title_full |
Haploid origin of cork oak anther embryos detected by enzyme and RAPD gene markers |
title_fullStr |
Haploid origin of cork oak anther embryos detected by enzyme and RAPD gene markers |
title_full_unstemmed |
Haploid origin of cork oak anther embryos detected by enzyme and RAPD gene markers |
title_sort |
haploid origin of cork oak anther embryos detected by enzyme and rapd gene markers |
publisher |
University of Chicago Press |
publishDate |
2000 |
url |
http://hdl.handle.net/20.500.12792/4921 http://hdl.handle.net/10261/294816 |
work_keys_str_mv |
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