Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture
The objective of this study is to induce the nuclear DNA duplication of anther-derived embryos of cork oak (Quercus suber L.) to obtain doubled-haploid plants. Anther culture of this species produces a low percentage (7.78%) of spontaneous diploids, as assessed by flow cytometry. Therefore, three antimitotic agents, colchicine, oryzalin and amiprophos-methyl (APM), were applied in vitro to anther-derived cork oak haploid embryos from six genotypes at different concentrations and for different treatment durations. Antimitotic toxicity was determined by embryo survival. Efficiency in inducing chromosome doubling of haploid embryos was evaluated by flow cytometry measurements and differences were observed between treatments. Nuclear DNA duplication and embryo survival of cork oak haploid embryos was most efficiently induced with oryzalin 0.01 mM for 48 h. Around 50% diploid embryos were obtained. The rate of chromosome duplication induced by APM 0.01 mM was also acceptable but lower than that induced by oryzalin, regardless of the duration of the treatment. Colchicine 1.3 or 8.8 mM was the least efficient, with the induction of necrosis and only a small rate of nuclear DNA duplication. © 2007 Elsevier GmbH. All rights reserved.
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Elsevier
2007
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Subjects: | Amiprophos-methyl, Colchicine, Flow cytometry, Oryzalin, Spontaneous diploidization, |
Online Access: | http://hdl.handle.net/20.500.12792/3532 http://hdl.handle.net/10261/294367 |
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dig-inia-es-10261-2943672023-02-20T10:38:01Z Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture Pintos, B. Manzanera, J. A. Bueno, M. A. Amiprophos-methyl Colchicine Flow cytometry Oryzalin Spontaneous diploidization The objective of this study is to induce the nuclear DNA duplication of anther-derived embryos of cork oak (Quercus suber L.) to obtain doubled-haploid plants. Anther culture of this species produces a low percentage (7.78%) of spontaneous diploids, as assessed by flow cytometry. Therefore, three antimitotic agents, colchicine, oryzalin and amiprophos-methyl (APM), were applied in vitro to anther-derived cork oak haploid embryos from six genotypes at different concentrations and for different treatment durations. Antimitotic toxicity was determined by embryo survival. Efficiency in inducing chromosome doubling of haploid embryos was evaluated by flow cytometry measurements and differences were observed between treatments. Nuclear DNA duplication and embryo survival of cork oak haploid embryos was most efficiently induced with oryzalin 0.01 mM for 48 h. Around 50% diploid embryos were obtained. The rate of chromosome duplication induced by APM 0.01 mM was also acceptable but lower than that induced by oryzalin, regardless of the duration of the treatment. Colchicine 1.3 or 8.8 mM was the least efficient, with the induction of necrosis and only a small rate of nuclear DNA duplication. © 2007 Elsevier GmbH. All rights reserved. 2023-02-20T10:38:01Z 2023-02-20T10:38:01Z 2007 journal article Journal of Plant Physiology 164(12): 1595-1604 (2007) 0176-1617 http://hdl.handle.net/20.500.12792/3532 http://hdl.handle.net/10261/294367 10.1016/j.jplph.2006.11.012 en none Elsevier |
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Amiprophos-methyl Colchicine Flow cytometry Oryzalin Spontaneous diploidization Amiprophos-methyl Colchicine Flow cytometry Oryzalin Spontaneous diploidization Pintos, B. Manzanera, J. A. Bueno, M. A. Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture |
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The objective of this study is to induce the nuclear DNA duplication of anther-derived embryos of cork oak (Quercus suber L.) to obtain doubled-haploid plants. Anther culture of this species produces a low percentage (7.78%) of spontaneous diploids, as assessed by flow cytometry. Therefore, three antimitotic agents, colchicine, oryzalin and amiprophos-methyl (APM), were applied in vitro to anther-derived cork oak haploid embryos from six genotypes at different concentrations and for different treatment durations. Antimitotic toxicity was determined by embryo survival. Efficiency in inducing chromosome doubling of haploid embryos was evaluated by flow cytometry measurements and differences were observed between treatments. Nuclear DNA duplication and embryo survival of cork oak haploid embryos was most efficiently induced with oryzalin 0.01 mM for 48 h. Around 50% diploid embryos were obtained. The rate of chromosome duplication induced by APM 0.01 mM was also acceptable but lower than that induced by oryzalin, regardless of the duration of the treatment. Colchicine 1.3 or 8.8 mM was the least efficient, with the induction of necrosis and only a small rate of nuclear DNA duplication. © 2007 Elsevier GmbH. All rights reserved. |
format |
journal article |
topic_facet |
Amiprophos-methyl Colchicine Flow cytometry Oryzalin Spontaneous diploidization |
author |
Pintos, B. Manzanera, J. A. Bueno, M. A. |
author_facet |
Pintos, B. Manzanera, J. A. Bueno, M. A. |
author_sort |
Pintos, B. |
title |
Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture |
title_short |
Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture |
title_full |
Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture |
title_fullStr |
Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture |
title_full_unstemmed |
Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture |
title_sort |
antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture |
publisher |
Elsevier |
publishDate |
2007 |
url |
http://hdl.handle.net/20.500.12792/3532 http://hdl.handle.net/10261/294367 |
work_keys_str_mv |
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