An efficient system to establish biopsy-derived trophoblastic cell lines from bovine embryos

Trophoblastic cells play a crucial role in implantation and placentogenesis and can be used as a model to provide substantial information on the peri-implantation period. Unfortunately, there are few cell lines for this purpose in cattle because of the difficulty of raising successive cell stocks in the long-term. Our results show that the combination of a monolayer culture system in microdrops on a surface treated with gelatin and the employment of conditioned media from mouse embryonic fibroblasts support the growth of bovine trophoblastic cells lines from an embryo biopsy. Expression profiles of mononucleate- and binucleate-specific genes in established trophoblastic cells lines represented various stages of gestation. Moreover, the ability to expand trophoblastic cell lines for more than 2 yr together with pluripotency-related gene expression patterns revealed certain self-renewal capacity. In summary, we have developed a system to expand in vitro trophoblastic cells from an embryo biopsy that solves the limitations of using amplified DNA from a small number of cells for bovine embryo genotyping and epigenotyping and, on the other hand, facilitates the establishment of trophoblastic cell lines that can be useful as peri-implantation in vitro models. © 2014 by the Society for the Study of Reproduction, Inc.

Bibliographic Details

Main Authors:	Ramos Ibeas, Priscila, Calle, A., Pericuesta Camacho, Eva, Laguna-Barraza, R., Moros-Mora, R., Lopera-Vásquez, R., Maillo, V., Yáñez-Mó, M., Gutiérrez Adán, Alfonso, Rizos Dimitrios, Dimitrios, Ramírez De Paz, Miguel Ángel
Format:	artículo biblioteca
Language:	English
Published:	Oxford University Press 2014
Subjects:	Bovine, Camel, Conditioned media, Embryo biopsy, Embryo culture, Ruminants, Trophoblast, Trophoblastic cell lines,
Online Access:	http://hdl.handle.net/20.500.12792/3654 http://hdl.handle.net/10261/292787
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