Inactivation of Gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system
Inhibitory activity of reuterin (β-hydroxypropionaldehyde) combined with the antimicrobial peptide nisin or the lactoperoxidase system (LPOS) against food-borne Gram-negative pathogens in milk refrigerated at 4 and 8 °C was investigated. At 4 °C, reuterin (8 AU/ml) and LPOS were bactericidal against Salmonella enterica, Campylobacter jejuni, Aeromonas hydrophila and Yersinia enterocolitica, whereas the only effect recorded for nisin was a slight inhibition of Escherichia coli O157H7. At 8 °C, reuterin was bactericidal against all the Gram-negative pathogens studied. Same results were attained with LPOS, except for its effect on Y. enterocolitica which was only bacteriostatic. The combination of reuterin with nisin did not enhance the antimicrobial effect of reuterin. A strong synergistic bactericidal activity of reuterin in combination with LPOS on E. coli O157H7 and S. enterica was observed in milk at 4 °C, and against all the Gram-negative bacteria assayed in milk refrigerated at 8 °C. The application of both antimicrobials would be a useful means to inhibit pathogenic microorganisms, which may be present in milk due to postpasteurization contamination. © 2007 Springer-Verlag.
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Format: | artículo biblioteca |
Language: | English |
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Springer
2008
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Subjects: | Reuterin, Nisin, Lactoperoxidase system, Food-borne pathogens, Combined treatments, Synergism, |
Online Access: | http://hdl.handle.net/20.500.12792/1840 http://hdl.handle.net/10261/292589 |
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dig-inia-es-10261-2925892023-02-20T07:30:27Z Inactivation of Gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system Arques Orobón, Juan Luis Rodríguez Mínguez, Eva Núñez Gutiérrez, Manuel Medina, M. Reuterin Nisin Lactoperoxidase system Food-borne pathogens Combined treatments Synergism Inhibitory activity of reuterin (β-hydroxypropionaldehyde) combined with the antimicrobial peptide nisin or the lactoperoxidase system (LPOS) against food-borne Gram-negative pathogens in milk refrigerated at 4 and 8 °C was investigated. At 4 °C, reuterin (8 AU/ml) and LPOS were bactericidal against Salmonella enterica, Campylobacter jejuni, Aeromonas hydrophila and Yersinia enterocolitica, whereas the only effect recorded for nisin was a slight inhibition of Escherichia coli O157H7. At 8 °C, reuterin was bactericidal against all the Gram-negative pathogens studied. Same results were attained with LPOS, except for its effect on Y. enterocolitica which was only bacteriostatic. The combination of reuterin with nisin did not enhance the antimicrobial effect of reuterin. A strong synergistic bactericidal activity of reuterin in combination with LPOS on E. coli O157H7 and S. enterica was observed in milk at 4 °C, and against all the Gram-negative bacteria assayed in milk refrigerated at 8 °C. The application of both antimicrobials would be a useful means to inhibit pathogenic microorganisms, which may be present in milk due to postpasteurization contamination. © 2007 Springer-Verlag. 2023-02-20T07:30:27Z 2023-02-20T07:30:27Z 2008 artículo European Food Research and Technology 227: 77-82 (2008) 1438-2377 http://hdl.handle.net/20.500.12792/1840 http://hdl.handle.net/10261/292589 10.1007/s00217-007-0695-8 1438-2385 en none Springer |
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Reuterin Nisin Lactoperoxidase system Food-borne pathogens Combined treatments Synergism Reuterin Nisin Lactoperoxidase system Food-borne pathogens Combined treatments Synergism |
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Reuterin Nisin Lactoperoxidase system Food-borne pathogens Combined treatments Synergism Reuterin Nisin Lactoperoxidase system Food-borne pathogens Combined treatments Synergism Arques Orobón, Juan Luis Rodríguez Mínguez, Eva Núñez Gutiérrez, Manuel Medina, M. Inactivation of Gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system |
description |
Inhibitory activity of reuterin (β-hydroxypropionaldehyde) combined with the antimicrobial peptide nisin or the lactoperoxidase system (LPOS) against food-borne Gram-negative pathogens in milk refrigerated at 4 and 8 °C was investigated. At 4 °C, reuterin (8 AU/ml) and LPOS were bactericidal against Salmonella enterica, Campylobacter jejuni, Aeromonas hydrophila and Yersinia enterocolitica, whereas the only effect recorded for nisin was a slight inhibition of Escherichia coli O157H7. At 8 °C, reuterin was bactericidal against all the Gram-negative pathogens studied. Same results were attained with LPOS, except for its effect on Y. enterocolitica which was only bacteriostatic. The combination of reuterin with nisin did not enhance the antimicrobial effect of reuterin. A strong synergistic bactericidal activity of reuterin in combination with LPOS on E. coli O157H7 and S. enterica was observed in milk at 4 °C, and against all the Gram-negative bacteria assayed in milk refrigerated at 8 °C. The application of both antimicrobials would be a useful means to inhibit pathogenic microorganisms, which may be present in milk due to postpasteurization contamination. © 2007 Springer-Verlag. |
format |
artículo |
topic_facet |
Reuterin Nisin Lactoperoxidase system Food-borne pathogens Combined treatments Synergism |
author |
Arques Orobón, Juan Luis Rodríguez Mínguez, Eva Núñez Gutiérrez, Manuel Medina, M. |
author_facet |
Arques Orobón, Juan Luis Rodríguez Mínguez, Eva Núñez Gutiérrez, Manuel Medina, M. |
author_sort |
Arques Orobón, Juan Luis |
title |
Inactivation of Gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system |
title_short |
Inactivation of Gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system |
title_full |
Inactivation of Gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system |
title_fullStr |
Inactivation of Gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system |
title_full_unstemmed |
Inactivation of Gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system |
title_sort |
inactivation of gram-negative pathogens in refrigerated milk by reuterin in combination with nisin or the lactoperoxidase system |
publisher |
Springer |
publishDate |
2008 |
url |
http://hdl.handle.net/20.500.12792/1840 http://hdl.handle.net/10261/292589 |
work_keys_str_mv |
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_version_ |
1767603365825478656 |