The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin
Arabidopsis thaliana S-Phase Kinase-Associated Protein 2A (SKP2A) is an F-box protein that regulates the proteolysis of cell cycle transcription factors. The plant hormone auxin regulates multiple aspects of plant growth and development, including cell division. We found that auxin induces the ubiquitin-dependent degradation of SKP2A both in vivo and in vitro, suggesting that this hormone acts as a signal to trigger SKP2A proteolysis. In this article, we show that auxin binds directly and specifically to SKP2A. By TIR1-based superposition and docking analyzes, we identified an auxin binding site in SKP2A. Mutations in this binding site reduce the ability of SKP2A to bind to auxin and generate nondegradable SKP2A forms. In addition, these non-auxin binding proteins are unable to promote E2FC/DPB degradation in vivo or to induce cell division in the root meristem. Auxin binds to TIR1 to promote its interaction with the auxin/indole-3-acetic acid target proteins. Here, we show that auxin also enhanced the interaction between SKP2A and DPB. Finally, a mutation in SKP2A leads to auxinresistant root growth, an effect that is additive with the tir1-1 phenotype. Thus, our data indicate that SKP2A is an auxin binding protein that connects auxin signaling with cell division.
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2010
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dig-inia-es-10261-2908652023-02-17T12:30:16Z The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin Jurado, S. Abraham, Z. Manzano, C. López-Torrejón, G. Pacios, L. F. del Pozo, J. C. Arabidopsis thaliana S-Phase Kinase-Associated Protein 2A (SKP2A) is an F-box protein that regulates the proteolysis of cell cycle transcription factors. The plant hormone auxin regulates multiple aspects of plant growth and development, including cell division. We found that auxin induces the ubiquitin-dependent degradation of SKP2A both in vivo and in vitro, suggesting that this hormone acts as a signal to trigger SKP2A proteolysis. In this article, we show that auxin binds directly and specifically to SKP2A. By TIR1-based superposition and docking analyzes, we identified an auxin binding site in SKP2A. Mutations in this binding site reduce the ability of SKP2A to bind to auxin and generate nondegradable SKP2A forms. In addition, these non-auxin binding proteins are unable to promote E2FC/DPB degradation in vivo or to induce cell division in the root meristem. Auxin binds to TIR1 to promote its interaction with the auxin/indole-3-acetic acid target proteins. Here, we show that auxin also enhanced the interaction between SKP2A and DPB. Finally, a mutation in SKP2A leads to auxinresistant root growth, an effect that is additive with the tir1-1 phenotype. Thus, our data indicate that SKP2A is an auxin binding protein that connects auxin signaling with cell division. 2023-02-17T12:30:16Z 2023-02-17T12:30:16Z 2010 artículo Plant Cell 22: 3891-3904 (2010) 1040-4651 http://hdl.handle.net/20.500.12792/2244 http://hdl.handle.net/10261/290865 10.1105/tpc.110.078972 1532-298X en none Oxford University Press |
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Arabidopsis thaliana S-Phase Kinase-Associated Protein 2A (SKP2A) is an F-box protein that regulates the proteolysis of cell cycle transcription factors. The plant hormone auxin regulates multiple aspects of plant growth and development, including cell division. We found that auxin induces the ubiquitin-dependent degradation of SKP2A both in vivo and in vitro, suggesting that this hormone acts as a signal to trigger SKP2A proteolysis. In this article, we show that auxin binds directly and specifically to SKP2A. By TIR1-based superposition and docking analyzes, we identified an auxin binding site in SKP2A. Mutations in this binding site reduce the ability of SKP2A to bind to auxin and generate nondegradable SKP2A forms. In addition, these non-auxin binding proteins are unable to promote E2FC/DPB degradation in vivo or to induce cell division in the root meristem. Auxin binds to TIR1 to promote its interaction with the auxin/indole-3-acetic acid target proteins. Here, we show that auxin also enhanced the interaction between SKP2A and DPB. Finally, a mutation in SKP2A leads to auxinresistant root growth, an effect that is additive with the tir1-1 phenotype. Thus, our data indicate that SKP2A is an auxin binding protein that connects auxin signaling with cell division. |
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Jurado, S. Abraham, Z. Manzano, C. López-Torrejón, G. Pacios, L. F. del Pozo, J. C. |
spellingShingle |
Jurado, S. Abraham, Z. Manzano, C. López-Torrejón, G. Pacios, L. F. del Pozo, J. C. The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin |
author_facet |
Jurado, S. Abraham, Z. Manzano, C. López-Torrejón, G. Pacios, L. F. del Pozo, J. C. |
author_sort |
Jurado, S. |
title |
The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin |
title_short |
The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin |
title_full |
The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin |
title_fullStr |
The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin |
title_full_unstemmed |
The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin |
title_sort |
arabidopsis cell cycle f-box protein skp2a binds to auxin |
publisher |
Oxford University Press |
publishDate |
2010 |
url |
http://hdl.handle.net/20.500.12792/2244 http://hdl.handle.net/10261/290865 |
work_keys_str_mv |
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