Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development
Reactive oxygen species (ROS) are physiologically generated during mitochondrial respiration and are involved in several signaling mechanisms. However, under pathological conditions, the concentration of ROS may exceed the antioxidant scavenging systems and subsequently lead to cell damage. High ROS levels have been proven to be detrimental to spermatozoa and furthermore compromise sperm function through lipid peroxidation, protein damage, and DNA strand breakage. Although the oral administration of antioxidants has been demonstrated to improve the semen quality in subfertile men, it is still a matter of debate if it can positively influence fertilization outcome and embryo developmental competence. Studies carried out in suitable animal models could resolve these fundamental questions. Hence, the main aims of the present study were to evaluate (1) the effects of zinc, d-aspartate, and coenzyme Q10, included in the dietary supplement Genadis (Merck Serono), on bull sperm motility and DNA fragmentation; and (2) whether treated spermatozoa have a superior competence in fertilization and in supporting the development of healthy embryos. Our data indicate that this treatment prevents the loss of sperm motility and the rise in sperm DNA fragmentation over time. Moreover, blastocyst rate was found to be significantly higher in oocytes fertilized by treated spermatozoa, and these blastocysts harbored a significantly lower percentage of apoptotic cells. © 2014 Elsevier Inc.
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Language: | English |
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Elsevier
2014
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Subjects: | Bovine, Spermatozoa, Antioxidants, DNA damage, Embryo development, |
Online Access: | http://hdl.handle.net/20.500.12792/1994 http://hdl.handle.net/10261/289363 |
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dig-inia-es-10261-2893632023-02-15T09:45:17Z Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development Gualtieri, R. Barbato, V. Fiorentino, I. Braun, S. Rizos Dimitrios, Dimitrios Longobardi, S. Talevi, R. Bovine Spermatozoa Antioxidants DNA damage Embryo development Reactive oxygen species (ROS) are physiologically generated during mitochondrial respiration and are involved in several signaling mechanisms. However, under pathological conditions, the concentration of ROS may exceed the antioxidant scavenging systems and subsequently lead to cell damage. High ROS levels have been proven to be detrimental to spermatozoa and furthermore compromise sperm function through lipid peroxidation, protein damage, and DNA strand breakage. Although the oral administration of antioxidants has been demonstrated to improve the semen quality in subfertile men, it is still a matter of debate if it can positively influence fertilization outcome and embryo developmental competence. Studies carried out in suitable animal models could resolve these fundamental questions. Hence, the main aims of the present study were to evaluate (1) the effects of zinc, d-aspartate, and coenzyme Q10, included in the dietary supplement Genadis (Merck Serono), on bull sperm motility and DNA fragmentation; and (2) whether treated spermatozoa have a superior competence in fertilization and in supporting the development of healthy embryos. Our data indicate that this treatment prevents the loss of sperm motility and the rise in sperm DNA fragmentation over time. Moreover, blastocyst rate was found to be significantly higher in oocytes fertilized by treated spermatozoa, and these blastocysts harbored a significantly lower percentage of apoptotic cells. © 2014 Elsevier Inc. 2023-02-15T09:45:17Z 2023-02-15T09:45:17Z 2014 artículo Theriogenology 82: 592-598 (2014) 0093-691X http://hdl.handle.net/20.500.12792/1994 http://hdl.handle.net/10261/289363 10.1016/j.theriogenology.2014.05.028 1879-3231 en none Elsevier |
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Bovine Spermatozoa Antioxidants DNA damage Embryo development Bovine Spermatozoa Antioxidants DNA damage Embryo development |
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Bovine Spermatozoa Antioxidants DNA damage Embryo development Bovine Spermatozoa Antioxidants DNA damage Embryo development Gualtieri, R. Barbato, V. Fiorentino, I. Braun, S. Rizos Dimitrios, Dimitrios Longobardi, S. Talevi, R. Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development |
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Reactive oxygen species (ROS) are physiologically generated during mitochondrial respiration and are involved in several signaling mechanisms. However, under pathological conditions, the concentration of ROS may exceed the antioxidant scavenging systems and subsequently lead to cell damage. High ROS levels have been proven to be detrimental to spermatozoa and furthermore compromise sperm function through lipid peroxidation, protein damage, and DNA strand breakage. Although the oral administration of antioxidants has been demonstrated to improve the semen quality in subfertile men, it is still a matter of debate if it can positively influence fertilization outcome and embryo developmental competence. Studies carried out in suitable animal models could resolve these fundamental questions. Hence, the main aims of the present study were to evaluate (1) the effects of zinc, d-aspartate, and coenzyme Q10, included in the dietary supplement Genadis (Merck Serono), on bull sperm motility and DNA fragmentation; and (2) whether treated spermatozoa have a superior competence in fertilization and in supporting the development of healthy embryos. Our data indicate that this treatment prevents the loss of sperm motility and the rise in sperm DNA fragmentation over time. Moreover, blastocyst rate was found to be significantly higher in oocytes fertilized by treated spermatozoa, and these blastocysts harbored a significantly lower percentage of apoptotic cells. © 2014 Elsevier Inc. |
format |
artículo |
topic_facet |
Bovine Spermatozoa Antioxidants DNA damage Embryo development |
author |
Gualtieri, R. Barbato, V. Fiorentino, I. Braun, S. Rizos Dimitrios, Dimitrios Longobardi, S. Talevi, R. |
author_facet |
Gualtieri, R. Barbato, V. Fiorentino, I. Braun, S. Rizos Dimitrios, Dimitrios Longobardi, S. Talevi, R. |
author_sort |
Gualtieri, R. |
title |
Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development |
title_short |
Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development |
title_full |
Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development |
title_fullStr |
Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development |
title_full_unstemmed |
Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development |
title_sort |
treatment with zinc, d-aspartate, and coenzyme q10 protects bull sperm against damage and improves their ability to support embryo development |
publisher |
Elsevier |
publishDate |
2014 |
url |
http://hdl.handle.net/20.500.12792/1994 http://hdl.handle.net/10261/289363 |
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