Effects of bisphenol A exposure during cardiac cell differentiation

Effects of bisphenol A exposure during cardiac cell differentiation

12 Pág. Departamento de Reproducción animal

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Bibliographic Details
Main Authors: Escarda-Castro, Enrique, Herráez, María Paz, Lombó, Marta
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Language:English
Published: Elsevier 2021-10-01
Subjects:Bisphenol A, DNA damage, H9c2 cells, Histone acetylation, Oxidative stress,
Online Access:http://hdl.handle.net/10261/287995
http://dx.doi.org/10.13039/501100003329
https://api.elsevier.com/content/abstract/scopus_id/85107644402
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spelling dig-inia-es-10261-2879952024-05-15T20:37:39Z Effects of bisphenol A exposure during cardiac cell differentiation Escarda-Castro, Enrique Herráez, María Paz Lombó, Marta Ministerio de Economía y Competitividad (España) Escarda-Castro, Enrique [0000-0001-6661-9963] Herráez, María Paz [0000-0002-0028-3616] Lombó, Marta [0000-0002-2042-798X] Bisphenol A DNA damage H9c2 cells Histone acetylation Oxidative stress 12 Pág. Departamento de Reproducción animal Heart development requires a precise temporal regulation of gene expression in cardiomyoblasts. Therefore, the transcriptional changes in differentiating cells can lead to congenital heart diseases. Although the genetic mutations underlie most of these alterations, exposure to environmental contaminants, such as bisphenol A (BPA), has been recently considered as a risk factor as well. In this study we investigated the genotoxic and epigenotoxic effects of BPA throughout cardiomyocyte differentiation. H9c2 cells (rat myoblasts) were exposed to 10 and 30 μM BPA before and during the last two days of cardiac-driven differentiation. Then, we have analysed the phenotypic and molecular modifications (at transcriptional, genetic and epigenetic level). The results showed that treated myoblasts developed a skeletal muscle cell-like phenotype. The transcriptional changes induced by BPA in genes codifying proteins involved in heart differentiation and function depend on the window of exposure to BPA. The exposure before differentiation repressed the expression of heart transcription factors (Hand2 and Gata4), whereas exposure during differentiation reduced the expression of cardiac-specific genes (Tnnt2, Myom2, Sln, and Atp2a1). Additionally, significant effects were observed regarding DNA damage and histone acetylation levels after the two periods of BPA exposure: in cells exposed to the toxicant the percentage of DNA repair foci (formed by the co-localization of γH2AX and 53BP1) increased in a dose-dependent manner, whereas the treatment with the toxicant triggered a decrease in the epigenetic marks H3K9ac and H3K27ac. Our in vitro results reveal that BPA seriously interferes with the process of cardiomyocyte differentiation, which could be related to the reported in vivo effects of this toxicant on cardiogenesis. This work was funded by the Spanish Ministry of Economy and Competitiveness (Project AGL2014-53167-C3-3-R). We would like to thank Dr. Beatriz Martín-Fernández for helping us with the cell line establishment. Peer reviewed 2023-02-01T11:52:16Z 2023-02-01T11:52:16Z 2021-10-01 artículo http://purl.org/coar/resource_type/c_6501 Environmental Pollution 286: 117567 (2021) 0269-7491 http://hdl.handle.net/10261/287995 10.1016/j.envpol.2021.117567 http://dx.doi.org/10.13039/501100003329 34126515 2-s2.0-85107644402 https://api.elsevier.com/content/abstract/scopus_id/85107644402 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2014-53167-C3-3-R/ES/EFECTO DE CONTAMINANTES EMERGENTES EN CELULAS DE LA LINEA GERMINAL MASCULINA: CONTRIBUCION PATERNA AL DESARROLLO Y HERENCIA TRANSGENERACIONAL/ Environmental pollution (Barking, Essex : 1987) Publisher's version https://doi.org/10.1016/j.envpol.2021.117567 Sí open Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Bisphenol A
DNA damage
H9c2 cells
Histone acetylation
Oxidative stress
Bisphenol A
DNA damage
H9c2 cells
Histone acetylation
Oxidative stress
spellingShingle Bisphenol A
DNA damage
H9c2 cells
Histone acetylation
Oxidative stress
Bisphenol A
DNA damage
H9c2 cells
Histone acetylation
Oxidative stress
Escarda-Castro, Enrique
Herráez, María Paz
Lombó, Marta
Effects of bisphenol A exposure during cardiac cell differentiation
description 12 Pág. Departamento de Reproducción animal
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Escarda-Castro, Enrique
Herráez, María Paz
Lombó, Marta
format artículo
topic_facet Bisphenol A
DNA damage
H9c2 cells
Histone acetylation
Oxidative stress
author Escarda-Castro, Enrique
Herráez, María Paz
Lombó, Marta
author_sort Escarda-Castro, Enrique
title Effects of bisphenol A exposure during cardiac cell differentiation
title_short Effects of bisphenol A exposure during cardiac cell differentiation
title_full Effects of bisphenol A exposure during cardiac cell differentiation
title_fullStr Effects of bisphenol A exposure during cardiac cell differentiation
title_full_unstemmed Effects of bisphenol A exposure during cardiac cell differentiation
title_sort effects of bisphenol a exposure during cardiac cell differentiation
publisher Elsevier
publishDate 2021-10-01
url http://hdl.handle.net/10261/287995
http://dx.doi.org/10.13039/501100003329
https://api.elsevier.com/content/abstract/scopus_id/85107644402
work_keys_str_mv AT escardacastroenrique effectsofbisphenolaexposureduringcardiaccelldifferentiation
AT herraezmariapaz effectsofbisphenolaexposureduringcardiaccelldifferentiation
AT lombomarta effectsofbisphenolaexposureduringcardiaccelldifferentiation
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