Melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: An ovine in vitro model
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Elsevier
2022-07-01
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| Subjects: | Assisted reproduction, Cumulus cells, In vitro embryo production, Melatonin, Oocyte, Oocyte quality, Ovary preservation, |
| Online Access: | http://hdl.handle.net/10261/274812 http://dx.doi.org/10.13039/501100007480 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85129345580 |
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Assisted reproduction Cumulus cells In vitro embryo production Melatonin Oocyte Oocyte quality Ovary preservation Assisted reproduction Cumulus cells In vitro embryo production Melatonin Oocyte Oocyte quality Ovary preservation |
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Assisted reproduction Cumulus cells In vitro embryo production Melatonin Oocyte Oocyte quality Ovary preservation Assisted reproduction Cumulus cells In vitro embryo production Melatonin Oocyte Oocyte quality Ovary preservation Sánchez-Ajofrín, Irene Martín-Maestro, Alicia Medina-Chávez, Daniela-Alejandra Laborda-Gomariz, Juan Ángel Peris-Frau, Patricia Garde, José Julián Soler, Ana J. Melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: An ovine in vitro model |
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11 Pág. |
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Ministerio de Economía y Competitividad (España) |
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Ministerio de Economía y Competitividad (España) Sánchez-Ajofrín, Irene Martín-Maestro, Alicia Medina-Chávez, Daniela-Alejandra Laborda-Gomariz, Juan Ángel Peris-Frau, Patricia Garde, José Julián Soler, Ana J. |
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artículo |
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Assisted reproduction Cumulus cells In vitro embryo production Melatonin Oocyte Oocyte quality Ovary preservation |
| author |
Sánchez-Ajofrín, Irene Martín-Maestro, Alicia Medina-Chávez, Daniela-Alejandra Laborda-Gomariz, Juan Ángel Peris-Frau, Patricia Garde, José Julián Soler, Ana J. |
| author_sort |
Sánchez-Ajofrín, Irene |
| title |
Melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: An ovine in vitro model |
| title_short |
Melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: An ovine in vitro model |
| title_full |
Melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: An ovine in vitro model |
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Melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: An ovine in vitro model |
| title_full_unstemmed |
Melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: An ovine in vitro model |
| title_sort |
melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: an ovine in vitro model |
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Elsevier |
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2022-07-01 |
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http://hdl.handle.net/10261/274812 http://dx.doi.org/10.13039/501100007480 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85129345580 |
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dig-inia-es-10261-2748122024-05-18T20:41:37Z Melatonin rescues the development and quality of oocytes and cumulus cells after prolonged ovary preservation: An ovine in vitro model Sánchez-Ajofrín, Irene Martín-Maestro, Alicia Medina-Chávez, Daniela-Alejandra Laborda-Gomariz, Juan Ángel Peris-Frau, Patricia Garde, José Julián Soler, Ana J. Ministerio de Economía y Competitividad (España) European Commission Ministerio de Ciencia e Innovación (España) Universidad de Castilla La Mancha Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Sánchez-Ajofrín, Irene [0000-0002-8232-3940] Martín-Maestro, Alicia [0000-0003-2579-6845] Medina-Chávez, Daniela Alejandra [0000-0001-6942-0829] Laborda-Gomariz, Juan Ángel [0000-0002-3788-1476] Peris-Frau, Patricia, [0000-0001-6266-0635] Garde, José Julián, [0000-0002-3667-6518] Soler, Ana J. [0000-0002-6429-5157] Assisted reproduction Cumulus cells In vitro embryo production Melatonin Oocyte Oocyte quality Ovary preservation 11 Pág. The preservation of ovaries beyond 7 h dramatically decreases the developmental potential of oocytes to reach the blastocyst stage during in vitro embryo production. Here we investigated the protective effects of melatonin in the ovarian preservation solution after prolonged storage (7 h) in ovine as an animal model. Slaughterhouse adult sheep ovaries were preserved in saline solution for 2 h (Control) and 7 h (Control stress), and with melatonin for 7 h and at different concentrations (Melatonin 10-3, 10-5, 10-7, 10-9, and 10-11 M). First, the fertilizing ability, embryo development rates, and blastocyst quality were investigated. Notably, a concentration of 10-9 M melatonin showed the greatest number (p < 0.05) of blastocysts produced after 7 h of ovary storage (24.75 ± 1.57%) and was comparable (p > 0.05) to that obtained after just 2 h of storage in the untreated Control (30.77 ± 1.57%). Then, oocyte quality parameters showed that, compared to Control stress, Melatonin actively reduced intracellular ROS content, caspase-3 activity, DNA fragmentation, and the abundance of pro-apoptotic transcripts BAX and CASP3, while increasing that of GDF9 and GPX1. In cumulus cells, flow cytometry results showed that melatonin decreased apoptosis and increased mitochondrial activity (p < 0.05). In addition, there was a greater (p < 0.05) abundance of HAS2, STAR, and PTGS mRNA transcripts in Melatonin compared to Control stress. These findings reveal a melatonin-mediated developmental rescue of oocytes against ischemic damage during ovary preservation which represents a promising strategy for successfully producing embryos when prolonged ovarian transport times are required. This research was funded by the Spanish Ministry of Economy and Competitiveness, grant number “AGL2017-89017-R”. P.P.-F. and D. A. M.-Ch. received a contract of “Plan Propio de la UCLM” confinaced by the European Social Fund. A.M.-M. received a predoctoral contract by MICINN confinaced by the European Social Fund (PRE2018-084837). I.S-A received a postdoctoral contract of “Plan Propio de la UCLM” confinaced by the European Social Fund. Peer reviewed 2022-07-05T11:39:29Z 2022-07-05T11:39:29Z 2022-07-01 artículo http://purl.org/coar/resource_type/c_6501 Theriogenology 186: 1-11 (2022) 0093-691X http://hdl.handle.net/10261/274812 10.1016/j.theriogenology.2022.04.001 1879-3231 http://dx.doi.org/10.13039/501100007480 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 35421773 2-s2.0-85129345580 https://api.elsevier.com/content/abstract/scopus_id/85129345580 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89017-R/ES/INCREMENTO EN LA OBTENCION DE EMBRIONES IN VITRO EN PEQUEÑOS RUMIANTES MEDIANTE LA MODIFICACION EN EL PROTOCOLO DE FECUNDACION IN VITRO: EL CIERVO Y EL OVINO COMO MODELOS/ info:eu-repo/grantAgreement/MICINN//PRE2018-084837 Theriogenology Publisher's version https://doi.org/10.1016/j.theriogenology.2022.04.001 Sí open Elsevier |