CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2022-05-05
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| Subjects: | CCR7, CD4 T cells, CD9, IFN-γ, Memory, Swine, |
| Online Access: | http://hdl.handle.net/10261/270603 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85129911259 |
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dig-inia-es-10261-2706032024-10-27T21:43:37Z CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells Álvarez, Belén Revilla Calvo, Concepción Moreno, Sara Jiménez-Marín, Ángeles Ramos, Elena Martínez De la Riva, Paloma Poderoso, Teresa Garrido Jurado, Juan José Ezquerra Martínez, Ángel Domínguez, Javier Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Álvarez, Belén [0000-0003-0426-0179] Revilla Calvo, Concepción [0000-0002-8197-4186] Jiménez-Marín, Ángeles [0000-0001-6546-9174] Martínez De la Riva, Paloma [0000-0003-3097-022X] Poderoso, Teresa [0000-0002-1406-000X] Garrido, Juan José [0000-0001-6592-2231] Ezquerra Martínez, Ángel [0000-0002-1824-5087] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] CCR7 CD4 T cells CD9 IFN-γ Memory Swine 9 Pág. In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows to distinguish at least five main CD4+ T cell subsets. Analysis on these subsets of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers depicts a progressive model of CD4+ T cell development. CD4+ 2E3+ CD8α- CD9- cells are the least differentiated population of naïve cells, whereas the CD4+ 2E3- CD8α+CD9+ and CD4+ 2E3- CD8α+ CD9- cells display phenotypic features of central and effector memory T helper cells, respectively. The latter subsets were able to produce IFN-γ after polyclonal activation with PMA/Ionomycin; however, in vitro virus-specific IFN-γ production of PBMCs collected at 38-44 days after pseudorabies virus vaccination was dominated by cells with a CD9+ phenotype. Therefore, CD9 appears to be a useful marker to investigate CD4+ T cell heterogeneity in swine. This work was supported by grant PID2019-109857RB-I00 funded by MCIN/AEI/10.13039/501100011033. ERDF A way of making Europe. Peer reviewed 2022-05-26T09:39:19Z 2022-05-26T09:39:19Z 2022-05-05 artículo http://purl.org/coar/resource_type/c_6501 Developmental and Comparative Immunology 133: 104431 (2022) 0145-305X http://hdl.handle.net/10261/270603 10.1016/j.dci.2022.104431 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 35526640 2-s2.0-85129911259 https://api.elsevier.com/content/abstract/scopus_id/85129911259 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109857RB-I00/ES/EVALUACION DEL USO DE ANTICUERPOS MONOCLONALES PARA DIRIGIR ANTIGENO A CELULAS PRESENTADORAS DE ANTIGENO COMO ESTRATEGIA PARA MEJORAR LA INMUNOGENICIDAD DE VACUNAS EN EL CERDO/ Developmental and comparative immunology Publisher's version https://doi.org/10.1016/j.dci.2022.104431 Sí open Elsevier |
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CCR7 CD4 T cells CD9 IFN-γ Memory Swine CCR7 CD4 T cells CD9 IFN-γ Memory Swine |
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CCR7 CD4 T cells CD9 IFN-γ Memory Swine CCR7 CD4 T cells CD9 IFN-γ Memory Swine Álvarez, Belén Revilla Calvo, Concepción Moreno, Sara Jiménez-Marín, Ángeles Ramos, Elena Martínez De la Riva, Paloma Poderoso, Teresa Garrido Jurado, Juan José Ezquerra Martínez, Ángel Domínguez, Javier CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells |
| description |
9 Pág. |
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Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Álvarez, Belén Revilla Calvo, Concepción Moreno, Sara Jiménez-Marín, Ángeles Ramos, Elena Martínez De la Riva, Paloma Poderoso, Teresa Garrido Jurado, Juan José Ezquerra Martínez, Ángel Domínguez, Javier |
| format |
artículo |
| topic_facet |
CCR7 CD4 T cells CD9 IFN-γ Memory Swine |
| author |
Álvarez, Belén Revilla Calvo, Concepción Moreno, Sara Jiménez-Marín, Ángeles Ramos, Elena Martínez De la Riva, Paloma Poderoso, Teresa Garrido Jurado, Juan José Ezquerra Martínez, Ángel Domínguez, Javier |
| author_sort |
Álvarez, Belén |
| title |
CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells |
| title_short |
CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells |
| title_full |
CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells |
| title_fullStr |
CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells |
| title_full_unstemmed |
CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells |
| title_sort |
cd9 expression in porcine blood cd4+ t cells delineates two subsets with phenotypic characteristics of central and effector memory cells |
| publisher |
Elsevier |
| publishDate |
2022-05-05 |
| url |
http://hdl.handle.net/10261/270603 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85129911259 |
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