Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes
11 páginas, 3 tablas, 2 figuras.
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| Language: | English |
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American Dairy Science Association
2014-02
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| Subjects: | δ9-desaturase, Endogenous synthesis, Mammary gland, Sheep, |
| Online Access: | http://hdl.handle.net/10261/92040 http://dx.doi.org/10.13039/501100003329 |
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dig-igm-es-10261-920402021-03-18T16:13:21Z Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes Frutos, Pilar Toral, Pablo G. Ramos Morales, Eva Shingfield, Kevin J. Belenguer, Álvaro Hervás, Gonzalo Ministerio de Economía y Competitividad (España) δ9-desaturase Endogenous synthesis Mammary gland Sheep 11 páginas, 3 tablas, 2 figuras. Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, consistent with an inhibition of stearoyl-coenzyme A desaturase (SCD) activity, but it remains unclear whether other ruminant species are also affected. The present study examined the effects of oral administration of Co acetate on intake, rumen function, and milk production and fatty acid (FA) composition in sheep. Twenty lactating Assaf ewes were allocated into 1 of 4 groups and used in a continuous randomized block design that involved a 15-d adaptation, a 6-d treatment, and a 10-d posttreatment period. During the treatment period, animals received an oral drench supplying 0 (control), 3 (Co3), 6 (Co6), and 9 (Co9) mg of Co/kg of BW per day, administered in 3 equal doses at 8-h intervals. Cobalt acetate had no influence on intake or milk fat and protein concentrations, whereas treatments Co6 and Co9 tended to lower milk yield. Results on rumen parameters showed no effects on rumen fermentation, FA composition, or bacterial community structure. Administration of Co acetate decreased milk concentrations of FA containing a cis -9 double bond and SCD product:substrate ratios, consistent with an inhibition of SCD activity in the ovine mammary gland. Temporal changes in milk fat composition indicated that the effects of treatments were evident within 3d of dosing, with further changes being apparent after 6d and reverting to pretreatment values by d 6 after administration. Effect on milk FA composition did not differ substantially in response to incremental doses of Co acetate. On average, Co decreased milk cis-9 10:1/10:0, cis-9 12:1/12:0, cis- 9 14:1/14:0, cis-9 16:1/16:0, cis-9 17:1/17:0, cis-9 18:1/18:0, and cis-9,trans-11 18:2/trans-11 18:1 concentration ratios by 30, 32, 38, 33, 21, 24, and 25%, respectively. Changes in milk fat cis-9 10:1, cis-9 12:1, and cis-9 14:1 concentrations to Co treatment indicated that 51% of cis-9 18:1 and cis-9,trans-11 18:2 secreted in milk originated from δ9-desaturation. In conclusion, results demonstrated the potential of oral Co administration for the estimation of endogenous synthesis of FA containing a cis-9 double bond in the mammary gland of lactating ruminants. Indirect comparisons suggest that the effects of Co differ between sheep and cattle. This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO, Madrid, Spain; AGL2011-23700). Peer reviewed 2014-02-19T12:10:22Z 2014-02-19T12:10:22Z 2014-02 artículo http://purl.org/coar/resource_type/c_6501 Journal of Dairy Science 97(2) : 1036-1046 (2014) 0022-0302 http://hdl.handle.net/10261/92040 10.3168/jds.2013-7327 http://dx.doi.org/10.13039/501100003329 en http://dx.doi.org/10.3168/jds.2013-7327 open American Dairy Science Association |
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δ9-desaturase Endogenous synthesis Mammary gland Sheep δ9-desaturase Endogenous synthesis Mammary gland Sheep |
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δ9-desaturase Endogenous synthesis Mammary gland Sheep δ9-desaturase Endogenous synthesis Mammary gland Sheep Frutos, Pilar Toral, Pablo G. Ramos Morales, Eva Shingfield, Kevin J. Belenguer, Álvaro Hervás, Gonzalo Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes |
| description |
11 páginas, 3 tablas, 2 figuras. |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Frutos, Pilar Toral, Pablo G. Ramos Morales, Eva Shingfield, Kevin J. Belenguer, Álvaro Hervás, Gonzalo |
| format |
artículo |
| topic_facet |
δ9-desaturase Endogenous synthesis Mammary gland Sheep |
| author |
Frutos, Pilar Toral, Pablo G. Ramos Morales, Eva Shingfield, Kevin J. Belenguer, Álvaro Hervás, Gonzalo |
| author_sort |
Frutos, Pilar |
| title |
Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes |
| title_short |
Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes |
| title_full |
Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes |
| title_fullStr |
Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes |
| title_full_unstemmed |
Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes |
| title_sort |
oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme a desaturase in lactating ewes |
| publisher |
American Dairy Science Association |
| publishDate |
2014-02 |
| url |
http://hdl.handle.net/10261/92040 http://dx.doi.org/10.13039/501100003329 |
| work_keys_str_mv |
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