Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity
5 páginas, 1 figura, 1 tabla.
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| Format: | artículo biblioteca |
| Language: | English |
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Blackwell Publishing
2011
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| Subjects: | Ascaris suum, Haemoglobin, Vaccination, White spots, |
| Online Access: | http://hdl.handle.net/10261/60012 |
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dig-igm-es-10261-600122021-04-13T15:13:18Z Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity Vlaminck, J. Martínez Valladares, María Dewilde, S. Moens, L. Tilleman, K. Deforce, D. Urban, J. Claerebout, E. Vercruysse, J. Geldhof, Peter Ascaris suum Haemoglobin Vaccination White spots 5 páginas, 1 figura, 1 tabla. To determine whether purified Ascaris suum haemoglobin (AsHb) is a suitable vaccine candidate for the control of Ascaris infections, pigs were vaccinated with AsHb in combination with QuilA adjuvant and challenged with A. suum eggs. The number of liver lesions and worms in the intestine was assessed on day 14, 28 and 56 post-infection (p.i.). No significant differences were found in the number of worms recovered between vaccinated and control pigs on any of these days. However, significantly more white spots were counted on the livers of vaccinated pigs on day 14 (+86%) and day 28 (+118%) p.i. compared with nonvaccinated controls. To investigate whether the increased immunoreactivity against the liver stage L3s in vaccinated pigs was triggered by and directed against AsHb, the transcription and expression of AsHb in this larval life stage was analysed by RT-PCR and immunoblotting. The results showed that neither the AsHb transcript nor protein was detectable in freshly hatched L3. However, the immunoblot analysis showed that vaccination with AsHb resulted in the production of antibodies binding to several other antigens of the L3, suggesting that these might be involved in the increased white spot development. This research was funded by a Ph.D. grant of the ‘Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) and a grant of the Fund for Scientific Research – Flanders (Belgium) (F.W.O. – Vlaanderen). PG is a postdoctoral fellow of the Fund for Scientific Research – Flanders (Belgium) (F.W.O. – Vlaanderen). Peer reviewed 2012-11-12T12:42:28Z 2012-11-12T12:42:28Z 2011 artículo http://purl.org/coar/resource_type/c_6501 Parasite Immunology 33: 250-254 (2011) 0141-9838 http://hdl.handle.net/10261/60012 10.1111/j.1365-3024.2010.01274.x en http://dx.doi.org/10.1111/j.1365-3024.2010.01274.x open Blackwell Publishing |
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| tag |
biblioteca |
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Europa del Sur |
| libraryname |
Biblioteca del IGM España |
| language |
English |
| topic |
Ascaris suum Haemoglobin Vaccination White spots Ascaris suum Haemoglobin Vaccination White spots |
| spellingShingle |
Ascaris suum Haemoglobin Vaccination White spots Ascaris suum Haemoglobin Vaccination White spots Vlaminck, J. Martínez Valladares, María Dewilde, S. Moens, L. Tilleman, K. Deforce, D. Urban, J. Claerebout, E. Vercruysse, J. Geldhof, Peter Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity |
| description |
5 páginas, 1 figura, 1 tabla. |
| format |
artículo |
| topic_facet |
Ascaris suum Haemoglobin Vaccination White spots |
| author |
Vlaminck, J. Martínez Valladares, María Dewilde, S. Moens, L. Tilleman, K. Deforce, D. Urban, J. Claerebout, E. Vercruysse, J. Geldhof, Peter |
| author_facet |
Vlaminck, J. Martínez Valladares, María Dewilde, S. Moens, L. Tilleman, K. Deforce, D. Urban, J. Claerebout, E. Vercruysse, J. Geldhof, Peter |
| author_sort |
Vlaminck, J. |
| title |
Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity |
| title_short |
Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity |
| title_full |
Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity |
| title_fullStr |
Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity |
| title_full_unstemmed |
Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity |
| title_sort |
immunizing pigs with ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity |
| publisher |
Blackwell Publishing |
| publishDate |
2011 |
| url |
http://hdl.handle.net/10261/60012 |
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1777663912047542272 |