Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression
13 páginas, 3 tablas, 2 figuras.
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Multidisciplinary Digital Publishing Institute
2021
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| Subjects: | Intelectin 2, Biomarkers, Immunohistochemistry, Ileocecal valve, Mycobacterium avium subsp. paratuberculosis, Paratuberculosis, |
| Online Access: | http://hdl.handle.net/10261/243568 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 |
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dig-igm-es-10261-2435682021-06-15T11:57:39Z Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression Blanco-Vázquez, C. Balseiro, Ana Alonso Hearn, M. Juste, Ramón A. Iglesias, N. Canive, M. Casais, R. CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) European Commission Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Balseiro Morales, Ana María [0000-0002-5121-7264] Intelectin 2 Biomarkers Immunohistochemistry Ileocecal valve Mycobacterium avium subsp. paratuberculosis Paratuberculosis 13 páginas, 3 tablas, 2 figuras. Paratuberculosis (PTB), a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), is responsible for important economic losses in the dairy industry. Our previous RNA-sequencing (RNA-Seq) analysis showed that bovine intelectin 2 (ITLN2) precursor gene was overexpressed in ileocecal valve (ICV) samples of animals with focal (log2 fold-change = 10.6) and diffuse (log2 fold-change = 6.8) PTB-associated lesions compared to animals without lesions. This study analyzes the potential use of ITLN2, a protein that has been described as fundamental in the innate immune response to infections, as a biomarker of MAP infection. The presence of ITLN2 was investigated by quantitative immunohistochemical analysis of ICV samples of 20 Holstein Friesian cows showing focal (n = 5), multifocal (n = 5), diffuse (n = 5) and no histological lesions (n = 5). Significant differences were observed in the mean number of ITLN2 immunostained goblet and Paneth cells between the three histopathological types and the control. The number of immunolabelled cells was higher in the focal histopathological type (116.9 ± 113.9) followed by the multifocal (108.7 ± 140.5), diffuse (76.5 ± 97.8) and control types (41.0 ± 81.3). These results validate ITLN2 as a post-mortem biomarker of disease progression. This work has been funded by the National Institute for Agricultural Research (INIA RTA2014-00009-C02), the Ministerio de Ciencia, Innovación y Universidades (MCIU) and the Agencia Estatal de Investigación (AEI) reference project RTI2018-094192-R-C22 (FEDER co-funded). Cristina Blanco Vázquez and Maria Canive were supported by a grant from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA, Spain). 2021-06-15T11:40:03Z 2021-06-15T11:40:03Z 2021 2021-06-15T11:40:03Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.3390/ani11051370 issn: 2076-2615 Animals 11: 1370 (2021) http://hdl.handle.net/10261/243568 10.3390/ani11051370 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTA2014-00009-C02 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094192-R-C22 Publisher's version http://dx.doi.org/10.3390/ani11051370 Sí open Multidisciplinary Digital Publishing Institute |
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Intelectin 2 Biomarkers Immunohistochemistry Ileocecal valve Mycobacterium avium subsp. paratuberculosis Paratuberculosis Intelectin 2 Biomarkers Immunohistochemistry Ileocecal valve Mycobacterium avium subsp. paratuberculosis Paratuberculosis |
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Intelectin 2 Biomarkers Immunohistochemistry Ileocecal valve Mycobacterium avium subsp. paratuberculosis Paratuberculosis Intelectin 2 Biomarkers Immunohistochemistry Ileocecal valve Mycobacterium avium subsp. paratuberculosis Paratuberculosis Blanco-Vázquez, C. Balseiro, Ana Alonso Hearn, M. Juste, Ramón A. Iglesias, N. Canive, M. Casais, R. Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression |
| description |
13 páginas, 3 tablas, 2 figuras. |
| author2 |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) |
| author_facet |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Blanco-Vázquez, C. Balseiro, Ana Alonso Hearn, M. Juste, Ramón A. Iglesias, N. Canive, M. Casais, R. |
| format |
artículo |
| topic_facet |
Intelectin 2 Biomarkers Immunohistochemistry Ileocecal valve Mycobacterium avium subsp. paratuberculosis Paratuberculosis |
| author |
Blanco-Vázquez, C. Balseiro, Ana Alonso Hearn, M. Juste, Ramón A. Iglesias, N. Canive, M. Casais, R. |
| author_sort |
Blanco-Vázquez, C. |
| title |
Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression |
| title_short |
Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression |
| title_full |
Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression |
| title_fullStr |
Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression |
| title_full_unstemmed |
Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression |
| title_sort |
bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10261/243568 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 |
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