Influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep
6 pages, 3 tables.--Contributed to: 12th Seminar of the FAO-CIHEAM Sub-NetWork on Sheep and Goat Nutrition. "Nutritional and foraging ecology of sheep and goats"(Thessalonica, Grecia, Oct 11-13, 2007).
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| Format: | artículo biblioteca |
| Language: | English |
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Centre international de hautes études agronomiques méditerranéennes
2009
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| Subjects: | Fibrolytic enzyme, Rumen fermentation, In situ degradation, Enzyme fibrolytique, Fermentation ruminale, Degradabilité in situ, |
| Online Access: | http://hdl.handle.net/10261/18343 |
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dig-igm-es-10261-183432021-03-18T16:54:33Z Influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep Influence de l'administration directe d'enzymes fibrolytiques sur l'activité fibrolytique dans le rumen du mouton Giraldo, L. A. Tejido, María L. Ranilla, María José Mantecón, Ángel R. Ramos, S. Carro Travieso, Mª Dolores Fibrolytic enzyme Rumen fermentation In situ degradation Enzyme fibrolytique Fermentation ruminale Degradabilité in situ 6 pages, 3 tables.--Contributed to: 12th Seminar of the FAO-CIHEAM Sub-NetWork on Sheep and Goat Nutrition. "Nutritional and foraging ecology of sheep and goats"(Thessalonica, Grecia, Oct 11-13, 2007). Six rumen-fistulated Merino sheep were used in a crossover design experiment to evaluate the effects of a direct-fed exogenous fibrolytic enzyme (12 g/d; ENZ) on ruminal fermentation. The enzyme presented endoglucanase and xylanase activities. Sheep were fed a mixed grass hay:concentrate (70:30; fresh matter basis) diet at a daily rate of 60 g/kg body weight 0.75. Concentrations of NH3-N and volatile fatty acids (VFA) and enzymatic activities were determined in ruminal samples at 0, 4 and 8 h after feeding. Samples of the grass hay were incubated in situ in the rumen of each sheep to measure dry matter (DM) and neutral detergent fibre (NDF) degradation. ENZ treatment did not affect (P>0.05) ruminal pH or concentrations of NH3-N and total VFA at any sampling time. In contrast, at 0 and 4 h after feeding, molar proportion of propionate tended (P<0.10) to be greater and acetate:propionate ratio tended (P<0.10) to be lower in ENZ-supplemented sheep. No effects of ENZ (P>0.10) were detected 8 h after feeding. Both the ruminally insoluble potentially degradable fraction (b) of grass hay DM and its fractional rate of degradation (c) were increased (P<0.05) by ENZ treatment. Supplementation with ENZ also increased (P=0.009 to 0.023) effective and potential degradability of grass hay DM and NDF. Ruminal fluid endoglucanase and xylanase activities were greater (P<0.05) at 4 h post-feeding in ENZ-supplementd sheep than in control animals. ENZ supplementation did not affecf (P=0.151 to 0,815) either exoglucanase or amylase activity at any sampling time. The authors wish to acknowledge the financial support received from the M.C.Y.T. of Spain (Proyect AGL2001-0130) and Junta de Castilla y León (LE040A05). L. A. Giraldo gratefully acknowledges receipt of a grant from the Fundación Carolina. Peer reviewed 2009-11-05T12:19:38Z 2009-11-05T12:19:38Z 2009 artículo http://purl.org/coar/resource_type/c_6501 Options Mediterranéennes, A, 85: 297-302 (2009) 1016-121X http://hdl.handle.net/10261/18343 en http://ressources.ciheam.org/om/pdf/a85/00801021.pdf open 5052971 bytes application/pdf Centre international de hautes études agronomiques méditerranéennes Food and Agriculture Organisation of the United Nations National Agricultural Research Foundation (Greece) |
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Fibrolytic enzyme Rumen fermentation In situ degradation Enzyme fibrolytique Fermentation ruminale Degradabilité in situ Fibrolytic enzyme Rumen fermentation In situ degradation Enzyme fibrolytique Fermentation ruminale Degradabilité in situ |
| spellingShingle |
Fibrolytic enzyme Rumen fermentation In situ degradation Enzyme fibrolytique Fermentation ruminale Degradabilité in situ Fibrolytic enzyme Rumen fermentation In situ degradation Enzyme fibrolytique Fermentation ruminale Degradabilité in situ Giraldo, L. A. Tejido, María L. Ranilla, María José Mantecón, Ángel R. Ramos, S. Carro Travieso, Mª Dolores Influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep |
| description |
6 pages, 3 tables.--Contributed to: 12th Seminar of the FAO-CIHEAM Sub-NetWork on Sheep and Goat Nutrition. "Nutritional and foraging ecology of sheep and goats"(Thessalonica, Grecia, Oct 11-13, 2007). |
| format |
artículo |
| topic_facet |
Fibrolytic enzyme Rumen fermentation In situ degradation Enzyme fibrolytique Fermentation ruminale Degradabilité in situ |
| author |
Giraldo, L. A. Tejido, María L. Ranilla, María José Mantecón, Ángel R. Ramos, S. Carro Travieso, Mª Dolores |
| author_facet |
Giraldo, L. A. Tejido, María L. Ranilla, María José Mantecón, Ángel R. Ramos, S. Carro Travieso, Mª Dolores |
| author_sort |
Giraldo, L. A. |
| title |
Influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep |
| title_short |
Influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep |
| title_full |
Influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep |
| title_fullStr |
Influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep |
| title_full_unstemmed |
Influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep |
| title_sort |
influence of direct-fed exogenous fibrolytic enzyme on ruminal fibrolityc activity in sheep |
| publisher |
Centre international de hautes études agronomiques méditerranéennes |
| publishDate |
2009 |
| url |
http://hdl.handle.net/10261/18343 |
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