Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes
11 páginas, 2 Figuras, 2 Tablas.
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EDP Sciences
2018
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| Online Access: | http://hdl.handle.net/10261/167590 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100014180 |
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dig-igm-es-10261-1675902021-12-27T15:55:14Z Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes Chitneedi, P. K. Suárez-Vega, Aroa Martínez Valladares, María Arranz, Juan José Gutiérrez-Gil, Beatriz Junta de Castilla y León European Commission Ministerio de Economía, Industria y Competitividad (España) 11 páginas, 2 Figuras, 2 Tablas. The present study exploited the RNA-seq technology to analyze the transcriptome of target tissues affected by the Teladorsagia circumcincta infection in two groups of adult ewes showing different statuses against gastrointestinal nematode (GIN) infection with the aim of identifying genes linked to GIN infection resistance in sheep. For this, based on the accumulated faecal egg count of 18 adult Churra ewes subjected to a first experimental infection with T. circumcincta, six ewes were classified as resistant and six others as susceptible to the infection. These 12 animals were dewormed and infected again. After humanitarian sacrifice of these 12 animals at day 7 post-infection, RNA samples were obtained from abomasal mucosa and lymph node tissues and RNA-Seq datasets were generated using an Illumina HiSeq 2000 sequencer. The distribution of the genes based on their expression level were very similar among the two different tissues and conditions. The differential expression analysis performed with two software (DESeq and EdgeR) only identified common differentially expressed genes (DEGs), a total of 106, in the lymph node samples which were considered as GIN-activated. The enrichment analysis performed for these GIN-activated genes identified some pathways related to cytokine-mediated immune response and the PPARG signaling pathway as well as disease terms related to inflammation and gastro-intestinal diseases as enriched. A systematic comparison with the results of previous studies confirmed the involvement of genes such as ITLN2, CLAC1 and galectins, in the immune mechanism activated against T. circumcincta in resistant sheep. Financial support for this project was received from the LE248U14 project of Junta de Castilla and León Government. P. K. Chitneedi is funded by a predoctoral fellowship from the Junta de Castilla and León Government and the European Social Fund. B Gutiérrez-Gil is funded by the “Ramón y Cajal” Programme (RYC-2012-10230) from the Spanish Ministry of Economy, Industry and Competitiveness (MINECO). M. Martínez-Valladares is also funded by the “Ramón y Cajal” Programme (RYC-2015-18368) from MINECO. Peer Reviewed 2018-07-12T10:54:22Z 2018-07-12T10:54:22Z 2018 2018-07-12T10:54:22Z artículo http://purl.org/coar/resource_type/c_6501 Veterinary Research 49: 39 (2018) 1297-9716 http://hdl.handle.net/10261/167590 10.1186/s13567-018-0534-x http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100014180 29703268 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2015-18368 Publisher's version http://dx.doi.org/10.1186/s13567-018-0534-x Sí open EDP Sciences |
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11 páginas, 2 Figuras, 2 Tablas. |
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Junta de Castilla y León |
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Junta de Castilla y León Chitneedi, P. K. Suárez-Vega, Aroa Martínez Valladares, María Arranz, Juan José Gutiérrez-Gil, Beatriz |
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artículo |
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Chitneedi, P. K. Suárez-Vega, Aroa Martínez Valladares, María Arranz, Juan José Gutiérrez-Gil, Beatriz |
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Chitneedi, P. K. Suárez-Vega, Aroa Martínez Valladares, María Arranz, Juan José Gutiérrez-Gil, Beatriz Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes |
| author_sort |
Chitneedi, P. K. |
| title |
Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes |
| title_short |
Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes |
| title_full |
Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes |
| title_fullStr |
Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes |
| title_full_unstemmed |
Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes |
| title_sort |
exploring the mechanisms of resistance to teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes |
| publisher |
EDP Sciences |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10261/167590 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100014180 |
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