Linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. A tool to compare water irrigation quality
The occurrence of contaminants of emerging concern (CECs) or heavy metals in reclaimed water used for agricultural irrigation may affect crop morphology and physiology. Here, we analyzed lettuce (Lactuca sativa) grown in outdoor lysimeters and irrigated with either tap water, used as a control, or reclaimed water: CAS-reclaimed water, an effluent from a conventional activated sludge system (CAS) followed by chlorination and sand filtration, or MBR-reclaimed water, an effluent from a membrane biological reactor (MBR). Chemical analyses identified seven CECs in the reclaimed waters, but only two of them were detected in lettuce (carbamazepine and azithromycin). Metabolomic and transcriptomic analyses revealed that irrigation with reclaimed water increased the concentrations of several crop metabolites (5-oxoproline, leucine, isoleucine, and fumarate) and of transcripts codifying for the plant stress-related genes Heat-Shock Protein 70 (HSP70) and Manganese Superoxide Dismutase (MnSOD). In both cases, MBR-water elicited the strongest response in lettuce, perhaps related to its comparatively high sodium adsorption ratio (4.5), rather than to its content in CECs or heavy metals. Our study indicates that crop metabolomic and transcriptomic profiles depend on the composition of irrigating water and that they could be used for testing the impact of water quality in agriculture.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2023-10-29
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Subjects: | Transcriptomics, Agriculture, Crops, Metabolomics, Pollutants, Reclaimed water, http://metadata.un.org/sdg/6, http://metadata.un.org/sdg/12, Ensure availability and sustainable management of water and sanitation for all, Responsible Consumption and Production, |
Online Access: | http://hdl.handle.net/10261/339201 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85176124125 |
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dig-idaea-es-10261-3392012024-05-17T20:40:39Z Linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. A tool to compare water irrigation quality Mansilla, Sylvia Escolà, Mònica Piña, Benjamín Portugal, José Iakovides, Iakovos C. Beretsou, Vasiliki G. Christou, Anastasis Fatta-Kassinos, Despo Bayona Termens, Josep María Matamoros, Víctor Ministerio de Ciencia e Innovación (España) Transcriptomics Agriculture Crops Metabolomics Pollutants Reclaimed water http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/12 Ensure availability and sustainable management of water and sanitation for all Responsible Consumption and Production The occurrence of contaminants of emerging concern (CECs) or heavy metals in reclaimed water used for agricultural irrigation may affect crop morphology and physiology. Here, we analyzed lettuce (Lactuca sativa) grown in outdoor lysimeters and irrigated with either tap water, used as a control, or reclaimed water: CAS-reclaimed water, an effluent from a conventional activated sludge system (CAS) followed by chlorination and sand filtration, or MBR-reclaimed water, an effluent from a membrane biological reactor (MBR). Chemical analyses identified seven CECs in the reclaimed waters, but only two of them were detected in lettuce (carbamazepine and azithromycin). Metabolomic and transcriptomic analyses revealed that irrigation with reclaimed water increased the concentrations of several crop metabolites (5-oxoproline, leucine, isoleucine, and fumarate) and of transcripts codifying for the plant stress-related genes Heat-Shock Protein 70 (HSP70) and Manganese Superoxide Dismutase (MnSOD). In both cases, MBR-water elicited the strongest response in lettuce, perhaps related to its comparatively high sodium adsorption ratio (4.5), rather than to its content in CECs or heavy metals. Our study indicates that crop metabolomic and transcriptomic profiles depend on the composition of irrigating water and that they could be used for testing the impact of water quality in agriculture. This study was carried out within the project "Decision support-based approach for sustainable water reuse application in agricultural production (DSWAP)" which has received funding from the Partnership on Research and Innovation in the Mediterranean Area (PRIMA) under grant agreement No 1822. PRIMA is supported by the European Union's Horizon 2020, The authors are also grateful for the support from the Spanish Ministry of Science, Innovation, and University (MCIN/AEI/10.13039/501100011033, grant RTI2018-096175-B-I00), and the Generalitat de Catalunya (2017SGR902). IDAEA-CSIC is a Centre of Excellence Severo Ochoa (Spanish Ministry of Science and Innovation, Project CEX2018-000794-S, ERDF A way of making Europe). Peer reviewed 2023-11-20T09:35:54Z 2023-11-20T09:35:54Z 2023-10-29 artículo http://purl.org/coar/resource_type/c_6501 Science of The Total Environment 908: 168182 (2023) 00489697 http://hdl.handle.net/10261/339201 10.1016/j.scitotenv.2023.168182 http://dx.doi.org/10.13039/501100004837 37907106 2-s2.0-85176124125 https://api.elsevier.com/content/abstract/scopus_id/85176124125 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MCIN/AEI/10.13039/501100011033 The Science of the total environment Publisher's version https://doi.org/10.1016/j.scitotenv.2023.168182 Sí open Elsevier |
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Transcriptomics Agriculture Crops Metabolomics Pollutants Reclaimed water http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/12 Ensure availability and sustainable management of water and sanitation for all Responsible Consumption and Production Transcriptomics Agriculture Crops Metabolomics Pollutants Reclaimed water http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/12 Ensure availability and sustainable management of water and sanitation for all Responsible Consumption and Production |
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Transcriptomics Agriculture Crops Metabolomics Pollutants Reclaimed water http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/12 Ensure availability and sustainable management of water and sanitation for all Responsible Consumption and Production Transcriptomics Agriculture Crops Metabolomics Pollutants Reclaimed water http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/12 Ensure availability and sustainable management of water and sanitation for all Responsible Consumption and Production Mansilla, Sylvia Escolà, Mònica Piña, Benjamín Portugal, José Iakovides, Iakovos C. Beretsou, Vasiliki G. Christou, Anastasis Fatta-Kassinos, Despo Bayona Termens, Josep María Matamoros, Víctor Linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. A tool to compare water irrigation quality |
description |
The occurrence of contaminants of emerging concern (CECs) or heavy metals in reclaimed water used for agricultural irrigation may affect crop morphology and physiology. Here, we analyzed lettuce (Lactuca sativa) grown in outdoor lysimeters and irrigated with either tap water, used as a control, or reclaimed water: CAS-reclaimed water, an effluent from a conventional activated sludge system (CAS) followed by chlorination and sand filtration, or MBR-reclaimed water, an effluent from a membrane biological reactor (MBR). Chemical analyses identified seven CECs in the reclaimed waters, but only two of them were detected in lettuce (carbamazepine and azithromycin). Metabolomic and transcriptomic analyses revealed that irrigation with reclaimed water increased the concentrations of several crop metabolites (5-oxoproline, leucine, isoleucine, and fumarate) and of transcripts codifying for the plant stress-related genes Heat-Shock Protein 70 (HSP70) and Manganese Superoxide Dismutase (MnSOD). In both cases, MBR-water elicited the strongest response in lettuce, perhaps related to its comparatively high sodium adsorption ratio (4.5), rather than to its content in CECs or heavy metals. Our study indicates that crop metabolomic and transcriptomic profiles depend on the composition of irrigating water and that they could be used for testing the impact of water quality in agriculture. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Mansilla, Sylvia Escolà, Mònica Piña, Benjamín Portugal, José Iakovides, Iakovos C. Beretsou, Vasiliki G. Christou, Anastasis Fatta-Kassinos, Despo Bayona Termens, Josep María Matamoros, Víctor |
format |
artículo |
topic_facet |
Transcriptomics Agriculture Crops Metabolomics Pollutants Reclaimed water http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/12 Ensure availability and sustainable management of water and sanitation for all Responsible Consumption and Production |
author |
Mansilla, Sylvia Escolà, Mònica Piña, Benjamín Portugal, José Iakovides, Iakovos C. Beretsou, Vasiliki G. Christou, Anastasis Fatta-Kassinos, Despo Bayona Termens, Josep María Matamoros, Víctor |
author_sort |
Mansilla, Sylvia |
title |
Linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. A tool to compare water irrigation quality |
title_short |
Linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. A tool to compare water irrigation quality |
title_full |
Linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. A tool to compare water irrigation quality |
title_fullStr |
Linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. A tool to compare water irrigation quality |
title_full_unstemmed |
Linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. A tool to compare water irrigation quality |
title_sort |
linking the use of reclaimed water to indicators of crop stress by metabolomic and transcriptomic analyses. a tool to compare water irrigation quality |
publisher |
Elsevier |
publishDate |
2023-10-29 |
url |
http://hdl.handle.net/10261/339201 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85176124125 |
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