Untangling comprehensive two-dimensional liquid chromatography data sets using regions of interest and multivariate curve resolution approaches

Data analysis remains a major challenge in the global application of comprehensive two-dimensional liquid chromatography (LC × LC). Advanced chemometric tools have been proposed to reduce the complexity of LC × LC datasets. In this work, key aspects of LC × LC are summarized from a chemometrics perspective. In particular, the recently developed ROIMCR method is proposed and adapted for LC × LC data analysis. First, this strategy consists of selecting of the Regions of Interest (ROI), in which data are filtered and compressed. Second, the resolution of the elution profiles of the sample constituents using the Multivariate Curve Resolution – Alternating Least Squares (MCR-ALS) method. A detailed overview of this recently developed tool and examples of its application in LC × LC are given, as well as pre-processing and post-processing tools to facilitate and complement the analysis of LC × LC data and the optimal interpretation of results.

Bibliographic Details

Main Authors:	Pérez-Cova, Miriam, Jaumot, Joaquim, Tauler, Romà
Other Authors:	Ministerio de Ciencia e Innovación (España)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2021-04-01
Subjects:	ROIMCR, Chemometrics, Data analysis, LC × LC, MCR-ALS,
Online Access:	http://hdl.handle.net/10261/266228 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85101469591
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