Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling
Animal behaviour is closely related to individual fitness, which allows animals to choose suitable mates or avoid predation. The central nervous system regulates many aspects of animal behaviour responses. Therefore, behavioural responses can be especially sensitive to compounds with a neurodevelopmental or neurofunctional mode of action. Phototactic behavioural changes against fish in the freshwater crustacean Daphnia magna have been the subject of many ecological investigations. The aim of this study was to identify which neurotransmitter systems modulate phototactic behaviour to fish kairomones. We used a positive phototactic D. magna clone (P132,85) that shows marked negative phototactism after exposure to fish kairomones. Treatments included up to 16 known agonists and antagonists of the serotonergic, cholinergic, dopaminergic, histaminergic, glutamatergic and GABAergic systems. It was hypothesized that many neurological signalling pathways may modulate D. magna phototactic behaviour to fish kairomones. A new custom-designed device with vertically oriented chambers was used, and changes in the preferred areas (bottom, middle, and upper areas) were analysed using groups of animals after 24 h of exposure to the selected substance(s). The results indicated that agonists of the muscarinic acetylcholine and GABAA receptors and their equi-effective mixture ameliorated the negative phototactic response to fish kairomones, whereas antagonists and their mixtures increased the negative phototactism to fish kairomones. Interestingly, inhibition of the muscarinic acetylcholine receptor abolished positive phototaxis, thus inducing the phototactic response to fish kairomones. Analysis of the profile of neurotransmitters and their related metabolites showed that the D. magna behavioural responses induced by fish depend on changes in the levels of acetylcholine, dopamine and GABA.
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Nature Publishing Group
2021-09-30
|
Subjects: | Neurophysiology, Behavioural ecology, |
Online Access: | http://hdl.handle.net/10261/253896 http://dx.doi.org/10.13039/501100004837 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-idaea-es-10261-253896 |
---|---|
record_format |
koha |
spelling |
dig-idaea-es-10261-2538962021-11-10T10:10:25Z Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling Bedrossiantz, Juliette Fuertes, Inmaculada Raldúa, Demetrio Barata Martí, Carlos Ministerio de Ciencia e Innovación (España) Raldúa, Demetrio [0000-0001-5256-1641] Neurophysiology Behavioural ecology Animal behaviour is closely related to individual fitness, which allows animals to choose suitable mates or avoid predation. The central nervous system regulates many aspects of animal behaviour responses. Therefore, behavioural responses can be especially sensitive to compounds with a neurodevelopmental or neurofunctional mode of action. Phototactic behavioural changes against fish in the freshwater crustacean Daphnia magna have been the subject of many ecological investigations. The aim of this study was to identify which neurotransmitter systems modulate phototactic behaviour to fish kairomones. We used a positive phototactic D. magna clone (P132,85) that shows marked negative phototactism after exposure to fish kairomones. Treatments included up to 16 known agonists and antagonists of the serotonergic, cholinergic, dopaminergic, histaminergic, glutamatergic and GABAergic systems. It was hypothesized that many neurological signalling pathways may modulate D. magna phototactic behaviour to fish kairomones. A new custom-designed device with vertically oriented chambers was used, and changes in the preferred areas (bottom, middle, and upper areas) were analysed using groups of animals after 24 h of exposure to the selected substance(s). The results indicated that agonists of the muscarinic acetylcholine and GABAA receptors and their equi-effective mixture ameliorated the negative phototactic response to fish kairomones, whereas antagonists and their mixtures increased the negative phototactism to fish kairomones. Interestingly, inhibition of the muscarinic acetylcholine receptor abolished positive phototaxis, thus inducing the phototactic response to fish kairomones. Analysis of the profile of neurotransmitters and their related metabolites showed that the D. magna behavioural responses induced by fish depend on changes in the levels of acetylcholine, dopamine and GABA. This work was funded by the Spanish Ministry of Science and Innovation projects (CTM2017-83242-R, PID2020-113371RB-C21) and the Centre of Excellence Severo Ochoa grant CEX2018-000794-S. Juliette Bedrossiantz and Inmaculada Fuertes were supported by the Spanish fellowships PRE2018-083513 and BES-2015-075023 co-financed by the European Social Fund, respectively. We thanks Luc De Meester for providing us the experimental clone and for his advice and suggestions on the experimental plan and Ms discussion. We also thank an anonymous reviewer for its constructive review. Peer reviewed 2021-11-08T10:19:24Z 2021-11-08T10:19:24Z 2021-09-30 artículo http://purl.org/coar/resource_type/c_6501 Scientific Reports 11: 19407 (2021) http://hdl.handle.net/10261/253896 10.1038/s41598-021-98886-w http://dx.doi.org/10.13039/501100004837 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTM2017-83242-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113371RB-C21 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CEX2018-000794-S Publisher's version https://doi.org/10.1038/s41598-021-98886-w Sí open Nature Publishing Group |
institution |
IDAEA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-idaea-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IDAEA España |
language |
English |
topic |
Neurophysiology Behavioural ecology Neurophysiology Behavioural ecology |
spellingShingle |
Neurophysiology Behavioural ecology Neurophysiology Behavioural ecology Bedrossiantz, Juliette Fuertes, Inmaculada Raldúa, Demetrio Barata Martí, Carlos Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling |
description |
Animal behaviour is closely related to individual fitness, which allows animals to choose suitable mates or avoid predation. The central nervous system regulates many aspects of animal behaviour responses. Therefore, behavioural responses can be especially sensitive to compounds with a neurodevelopmental or neurofunctional mode of action. Phototactic behavioural changes against fish in the freshwater crustacean Daphnia magna have been the subject of many ecological investigations. The aim of this study was to identify which neurotransmitter systems modulate phototactic behaviour to fish kairomones. We used a positive phototactic D. magna clone (P132,85) that shows marked negative phototactism after exposure to fish kairomones. Treatments included up to 16 known agonists and antagonists of the serotonergic, cholinergic, dopaminergic, histaminergic, glutamatergic and GABAergic systems. It was hypothesized that many neurological signalling pathways may modulate D. magna phototactic behaviour to fish kairomones. A new custom-designed device with vertically oriented chambers was used, and changes in the preferred areas (bottom, middle, and upper areas) were analysed using groups of animals after 24 h of exposure to the selected substance(s). The results indicated that agonists of the muscarinic acetylcholine and GABAA receptors and their equi-effective mixture ameliorated the negative phototactic response to fish kairomones, whereas antagonists and their mixtures increased the negative phototactism to fish kairomones. Interestingly, inhibition of the muscarinic acetylcholine receptor abolished positive phototaxis, thus inducing the phototactic response to fish kairomones. Analysis of the profile of neurotransmitters and their related metabolites showed that the D. magna behavioural responses induced by fish depend on changes in the levels of acetylcholine, dopamine and GABA. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Bedrossiantz, Juliette Fuertes, Inmaculada Raldúa, Demetrio Barata Martí, Carlos |
format |
artículo |
topic_facet |
Neurophysiology Behavioural ecology |
author |
Bedrossiantz, Juliette Fuertes, Inmaculada Raldúa, Demetrio Barata Martí, Carlos |
author_sort |
Bedrossiantz, Juliette |
title |
Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling |
title_short |
Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling |
title_full |
Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling |
title_fullStr |
Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling |
title_full_unstemmed |
Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling |
title_sort |
pharmacological modulation of fish-induced depth selection in d. magna: the role of cholinergic and gabaergic signalling |
publisher |
Nature Publishing Group |
publishDate |
2021-09-30 |
url |
http://hdl.handle.net/10261/253896 http://dx.doi.org/10.13039/501100004837 |
work_keys_str_mv |
AT bedrossiantzjuliette pharmacologicalmodulationoffishinduceddepthselectionindmagnatheroleofcholinergicandgabaergicsignalling AT fuertesinmaculada pharmacologicalmodulationoffishinduceddepthselectionindmagnatheroleofcholinergicandgabaergicsignalling AT ralduademetrio pharmacologicalmodulationoffishinduceddepthselectionindmagnatheroleofcholinergicandgabaergicsignalling AT baratamarticarlos pharmacologicalmodulationoffishinduceddepthselectionindmagnatheroleofcholinergicandgabaergicsignalling |
_version_ |
1777669552833822720 |