Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry

Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry

6 pages, 4 figures, 3 tables.-- PMID: 18004742 [PubMed].

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Main Authors: Pérez Solsona, Sandra, Farkas, Michael, Barceló, Damià, Aga, Diana S.
Format: artículo biblioteca
Language:English
Published: John Wiley & Sons 2007-11-14
Subjects:Glutathione conjugates, Mass spectrometry, Liquid chromatography, Stress-induced, Elucidation GSH, Stable drug-GSH, Biological systems,
Online Access:http://hdl.handle.net/10261/10658
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spelling dig-idaea-es-10261-106582020-07-06T12:54:45Z Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry Pérez Solsona, Sandra Farkas, Michael Barceló, Damià Aga, Diana S. Glutathione conjugates Mass spectrometry Liquid chromatography Stress-induced Elucidation GSH Stable drug-GSH Biological systems 6 pages, 4 figures, 3 tables.-- PMID: 18004742 [PubMed]. Glutathione S-transferases (GSTs) isolated from maize were used to catalyze the conjugation of glutathione (GSH) with chloroacetanilide herbicides, producing stable conjugates that were structurally characterized using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/QqToF-MS) and liquid chromatography/ion trap mass spectrometry (LC/IT-MS). Enzyme-mediated dechlorination of alachlor, metolachlor, and propachlor resulted during GSH conjugation as revealed by the mass spectra of the conjugates, which was confirmed by the loss of the chlorine isotopic signature and from high accurate mass measurements. Several fragmentation patterns in the mass spectra of the chloroacetanilide-GSH conjugates can be used to verify the identities of the enzyme reaction products, such as characteristic ions corresponding to the neutral loss of glutamic acid residue (129 Da) and water (18 Da) observed in the product ion spectrum. For the first time, data are presented showing detection of chloroacetanilides that are conjugated with two GSH molecules, in addition to the known single GSH conjugates. The work presented in this article was supported by the EU Project EMCO-INCO-CT-2004-509188) and by the Spanish Ministerio de Educación y Ciencia, Project EVITA (CTM2004-06255-CO3-01) and CEMAGUA (CGL2007-64551/HID). SP acknowledges a postdoctoral contract from I3P Program (Itinerario Integrado de Inserción Profesional); co-financed by CSIC and European Social Funds. Peer reviewed 2009-02-13T11:33:28Z 2009-02-13T11:33:28Z 2007-11-14 artículo http://purl.org/coar/resource_type/c_6501 Rapid Communication in Mass Spectrometry 21(24): 4017-4022 (2007) 0951-4198 http://hdl.handle.net/10261/10658 10.1002/rcm.3308 1097-0231 en http://dx.doi.org/10.1002/rcm.3308 none 162 bytes application/msword John Wiley & Sons
institution IDAEA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-idaea-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IDAEA España
language English
topic Glutathione conjugates
Mass spectrometry
Liquid chromatography
Stress-induced
Elucidation GSH
Stable drug-GSH
Biological systems
Glutathione conjugates
Mass spectrometry
Liquid chromatography
Stress-induced
Elucidation GSH
Stable drug-GSH
Biological systems
spellingShingle Glutathione conjugates
Mass spectrometry
Liquid chromatography
Stress-induced
Elucidation GSH
Stable drug-GSH
Biological systems
Glutathione conjugates
Mass spectrometry
Liquid chromatography
Stress-induced
Elucidation GSH
Stable drug-GSH
Biological systems
Pérez Solsona, Sandra
Farkas, Michael
Barceló, Damià
Aga, Diana S.
Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry
description 6 pages, 4 figures, 3 tables.-- PMID: 18004742 [PubMed].
format artículo
topic_facet Glutathione conjugates
Mass spectrometry
Liquid chromatography
Stress-induced
Elucidation GSH
Stable drug-GSH
Biological systems
author Pérez Solsona, Sandra
Farkas, Michael
Barceló, Damià
Aga, Diana S.
author_facet Pérez Solsona, Sandra
Farkas, Michael
Barceló, Damià
Aga, Diana S.
author_sort Pérez Solsona, Sandra
title Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry
title_short Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry
title_full Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry
title_fullStr Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry
title_full_unstemmed Characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry
title_sort characterization of glutathione conjugates of chloroacetanilide pesticides using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry
publisher John Wiley & Sons
publishDate 2007-11-14
url http://hdl.handle.net/10261/10658
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