Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea
8 p., 7 figures, 2 tables and references
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2011-03
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| Subjects: | Ammonium, N-(n-butyl) thiophosphoric triamide (NBPT), Urease inhibitor, Urea, |
| Online Access: | http://hdl.handle.net/10261/49621 |
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dig-idab-es-10261-496212016-09-07T10:24:34Z Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea NBPT implications on nitrogen metabolism Cruchaga, Saioa Artola, Ekhiñe Lasa, Berta Ariz, Idoia Irigoyen, Ignacio Morán, José F. Aparicio-Tejo, Pedro María Ammonium N-(n-butyl) thiophosphoric triamide (NBPT) Urease inhibitor Urea 8 p., 7 figures, 2 tables and references The application of urease inhibitors in conjunction with urea fertilizers as a means of reducing N loss due to ammonia volatilization requires an in-depth study of the physiological effects of these inhibitors on plants. The aim of this study was to determine how the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) affects N metabolism in pea and spinach. Plants were cultivated in pure hydroponic culture with urea as the sole N source. After 2 weeks of growth for pea, and 3 weeks for spinach, half of the plants received NBPT in their nutrient solution. Urease activity, urea and ammonium content, free amino acid composition and soluble protein were determined in leaves and roots at days 0, 1, 2, 4, 7 and 9, and the NBPT content in these tissues was determined 48 h after inhibitor application. The results suggest that the effects of NBPT on spinach and pea urease activity differ, with pea being most affected by this treatment, and that the NBPT absorbed by the plant caused a clear inhibition of the urease activity in pea leaf and roots. The high urea concentration observed in leaves was associated with the development of necrotic leaf margins, and was further evidence of NBPT inhibition in these plants. A decrease in the ammonium content in roots, where N assimilation mainly takes place, was also observed. Consequently, total amino acid contents were drastically reduced upon NBPT treatment, indicating a strong alteration of the N metabolism. Furthermore, the amino acid profile showed that amidic amino acids were major components of the reduced pool of amino acids. In contrast, NBPT was absorbed to a much lesser degree by spinach plants than pea plants (35% less) and did not produce a clear inhibition of urease activity in this species. This work was supported by the Spanish MICIIN (grant no. AGL2009-13339-CO2-02 [to P.A.T.]). S.C was supported by a doctoral fellowship from the Public University of Navarre. Peer reviewed 2012-05-14T09:37:31Z 2012-05-14T09:37:31Z 2011-03 artículo http://purl.org/coar/resource_type/c_6501 Journal of Plant Physiology 168(4): 329-336 (2011) 0176-1617 http://hdl.handle.net/10261/49621 10.1016/j.jplph.2010.07.024 1618-1328 en http://dx.doi.org/10.1016/j.jplph.2010.07.024 open Elsevier |
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Ammonium N-(n-butyl) thiophosphoric triamide (NBPT) Urease inhibitor Urea Ammonium N-(n-butyl) thiophosphoric triamide (NBPT) Urease inhibitor Urea |
| spellingShingle |
Ammonium N-(n-butyl) thiophosphoric triamide (NBPT) Urease inhibitor Urea Ammonium N-(n-butyl) thiophosphoric triamide (NBPT) Urease inhibitor Urea Cruchaga, Saioa Artola, Ekhiñe Lasa, Berta Ariz, Idoia Irigoyen, Ignacio Morán, José F. Aparicio-Tejo, Pedro María Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea |
| description |
8 p., 7 figures, 2 tables and references |
| format |
artículo |
| topic_facet |
Ammonium N-(n-butyl) thiophosphoric triamide (NBPT) Urease inhibitor Urea |
| author |
Cruchaga, Saioa Artola, Ekhiñe Lasa, Berta Ariz, Idoia Irigoyen, Ignacio Morán, José F. Aparicio-Tejo, Pedro María |
| author_facet |
Cruchaga, Saioa Artola, Ekhiñe Lasa, Berta Ariz, Idoia Irigoyen, Ignacio Morán, José F. Aparicio-Tejo, Pedro María |
| author_sort |
Cruchaga, Saioa |
| title |
Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea |
| title_short |
Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea |
| title_full |
Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea |
| title_fullStr |
Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea |
| title_full_unstemmed |
Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea |
| title_sort |
short term physiological implications of nbpt application on the n metabolism of pisum sativum and spinacea oleracea |
| publisher |
Elsevier |
| publishDate |
2011-03 |
| url |
http://hdl.handle.net/10261/49621 |
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