Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies

Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies

Trabajo presentado en el 49th Annual Meeting of the Society for Invertebrate Pathology: International Congress on Invertebrate Pathology and Microbial Control, celebrado en Tours (Francia), del 24 al 28 de julio de 2016

Saved in:
Bibliographic Details
Main Authors: Carballo, Arkaitz, Herrero, Salvador, Williams, Trevor G., Murillo, Rosa, Caballero, Primitivo
Format: comunicación de congreso biblioteca
Language:English
Published: 2016-07-24
Online Access:http://hdl.handle.net/10261/191985
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-idab-es-10261-191985
record_format koha
spelling dig-idab-es-10261-1919852019-10-02T01:10:04Z Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies Carballo, Arkaitz Herrero, Salvador Williams, Trevor G. Murillo, Rosa Caballero, Primitivo Trabajo presentado en el 49th Annual Meeting of the Society for Invertebrate Pathology: International Congress on Invertebrate Pathology and Microbial Control, celebrado en Tours (Francia), del 24 al 28 de julio de 2016 The Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) is a specific pathogen that can efficiently control this pest in greenhouse crops in southern Spain and elsewhere. Studies on persistent sublethal infections of S. exigua insect colonies revealed the presence of RNA viruses identified as two iflaviruses species (SeIV1 and SeIV2). SeMNPV and SeIV1/2 could be detected in both field and laboratory insect populations using PCR-based techniques. In this study, we evaluated the effect of SeIV1/2 co-infection on the insecticidal characteristics of SeMNPV, including pathogenicity, virulence and transmission traits. Bioassays were performed by simultaneously inoculating second instars with one of five SeMNPV occlusion body (OB) concentrations and one of the following four treatments: i) SeIV1; ii) SeIV2; iii) SeIV1+SeIV2; and iv) mock-infected control. Iflavirus co-inoculation consistently reduced SeMNPV median lethal concentrations (LC50) for S. exigua larvae compared to those infected with the SeMNPV alone. The speed of kill ofSeMNPV was similar in the presence or absence of the iflaviruses. Conversely, OB production upon death was significantly reduced due to a reduction in weight gain in iflavirus infected insects. Adults survivors of a sublethal SeMNPVOB treatment were examined for covert infection. SeMNPV DNA was found to be present at a high prevalence in all treatments (70-89 %), but viral load was quantified significantly less abundant in adults that had been previously co-inoculated with SeIV1. In conclusion, iflavirus co-infection increased the pathogenicity of SeMNPV OBs. We predict that SeMNPV-SeIV interactions might reduce insect-to-insect horizontal transmission under field conditions Peer reviewed 2019-10-01T11:45:04Z 2019-10-01T11:45:04Z 2016-07-24 comunicación de congreso http://purl.org/coar/resource_type/c_5794 49th Annual Meeting of the Society for Invertebrate Pathology (2016) http://hdl.handle.net/10261/191985 en Sí none
institution IDAB ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-idab-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IDAB España
language English
description Trabajo presentado en el 49th Annual Meeting of the Society for Invertebrate Pathology: International Congress on Invertebrate Pathology and Microbial Control, celebrado en Tours (Francia), del 24 al 28 de julio de 2016
format comunicación de congreso
author Carballo, Arkaitz
Herrero, Salvador
Williams, Trevor G.
Murillo, Rosa
Caballero, Primitivo
spellingShingle Carballo, Arkaitz
Herrero, Salvador
Williams, Trevor G.
Murillo, Rosa
Caballero, Primitivo
Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies
author_facet Carballo, Arkaitz
Herrero, Salvador
Williams, Trevor G.
Murillo, Rosa
Caballero, Primitivo
author_sort Carballo, Arkaitz
title Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies
title_short Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies
title_full Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies
title_fullStr Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies
title_full_unstemmed Spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) occlusion bodies
title_sort spodoptera exigua iflavirus co-inoculation alters the insecticidal properties of spodoptera exigua multiple nucleopolyhedrovirus (semnpv) occlusion bodies
publishDate 2016-07-24
url http://hdl.handle.net/10261/191985
work_keys_str_mv AT carballoarkaitz spodopteraexiguaiflaviruscoinoculationalterstheinsecticidalpropertiesofspodopteraexiguamultiplenucleopolyhedrovirussemnpvocclusionbodies
AT herrerosalvador spodopteraexiguaiflaviruscoinoculationalterstheinsecticidalpropertiesofspodopteraexiguamultiplenucleopolyhedrovirussemnpvocclusionbodies
AT williamstrevorg spodopteraexiguaiflaviruscoinoculationalterstheinsecticidalpropertiesofspodopteraexiguamultiplenucleopolyhedrovirussemnpvocclusionbodies
AT murillorosa spodopteraexiguaiflaviruscoinoculationalterstheinsecticidalpropertiesofspodopteraexiguamultiplenucleopolyhedrovirussemnpvocclusionbodies
AT caballeroprimitivo spodopteraexiguaiflaviruscoinoculationalterstheinsecticidalpropertiesofspodopteraexiguamultiplenucleopolyhedrovirussemnpvocclusionbodies
_version_ 1777663509317812224

Similar Items