Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University

Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University

Trabajo presentado en el KAAB International Symposium (Frontiers in Plant Science and Biotechnology), celebrado en Niigata (Japón) el 29 de septiembre de 2015.

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Main Authors: Fukushima, Mamiko, Almagro, Goizeder, Hanashiro, Isao, Pozueta Romero, Javier, Mitsui, Toshiaki, Itoh, Kimiko
Other Authors: Japan Society for the Promotion of Science
Format: comunicación de congreso biblioteca
Published: 2015-09
Online Access:http://hdl.handle.net/10261/142378
http://dx.doi.org/10.13039/501100001691
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spelling dig-idab-es-10261-1423782017-01-18T10:57:39Z Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University Fukushima, Mamiko Almagro, Goizeder Hanashiro, Isao Pozueta Romero, Javier Mitsui, Toshiaki Itoh, Kimiko Japan Society for the Promotion of Science Trabajo presentado en el KAAB International Symposium (Frontiers in Plant Science and Biotechnology), celebrado en Niigata (Japón) el 29 de septiembre de 2015. Natural storage polysaccharides, like as amylopectin and glycogen, vary in form and molecular size. Glycogen molecule is smaller in size and higher in branching degree than that of amylopectin. To develop a novel type of glycogen containing a long α-1, 4 glucosyl chain, we introduced rice granule-bound starch synthase I (GBSSI) gene in Escherichia coli and analyzed its expression and effect on glycogen synthesis, Rice GBSSI synthesizes not only amylose but also amylopectin, especially synthesizes extra-long chain, whose degrees of polymerization is over one hundred (1). We constructed the expression vector, pHis:SUMO:GBSSI, and confirmed that the expressed His:SUMO:GBSSI can synthesize extra-long chain in amylopectin in vitro. We transformed the pHis:SUMO:GBSSI into various E. coli strains, BW25113 and its mutants deficient in genes related to glycogen accumulation (Keio Collection)(2,3), and then examined effect of GBSSI expression on changes in nature and the amount of glycogen. Genes involved in Glycogen metabolism in E. coli are clustered in two apparently independent transcriptional units, glgBX (encoding branching enzyme GlgB and debranching enzyme GlgX) and glgCAP (encoding ADPG pyrophosphorylase GlgC, glycogen synthase GlgA and glycogen phosphorylase GlgP),. Both of ΔglgX andΔglgP mutants show increase in glycogen accumulation than BW25113, whileΔglgA mutant accumulates no glycogen at all. We use these three mutants,ΔglgX, ΔglgP, and ΔglgA, for this study. GBSSI expressing ΔglgX,ΔglgP, and BW25113 produced much more glycogen than non-expressing E. coli mutants, whereas the GBSSI expressing mutant ΔglgA did not produce the glycogen at all. The results indicate that the rice GBSSI does not complement the glycogen synthase deficiency by ΔglgA in E. coli. Our research also indicates that GBSSI can function not only in rice, but also in E. coli and affect its glycogen accumulation. Funding acknowledgements: This work was supported by KAAB project and Grant-in-Aid for Scientific Research, No. 15K00779, JSPS. Peer Reviewed 2017-01-11T11:46:20Z 2017-01-11T11:46:20Z 2015-09 2017-01-11T11:46:22Z comunicación de congreso http://purl.org/coar/resource_type/c_5794 KAAB International Symposium (2015) http://hdl.handle.net/10261/142378 http://dx.doi.org/10.13039/501100001691 Sí none
institution IDAB ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-idab-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IDAB España
description Trabajo presentado en el KAAB International Symposium (Frontiers in Plant Science and Biotechnology), celebrado en Niigata (Japón) el 29 de septiembre de 2015.
author2 Japan Society for the Promotion of Science
author_facet Japan Society for the Promotion of Science
Fukushima, Mamiko
Almagro, Goizeder
Hanashiro, Isao
Pozueta Romero, Javier
Mitsui, Toshiaki
Itoh, Kimiko
format comunicación de congreso
author Fukushima, Mamiko
Almagro, Goizeder
Hanashiro, Isao
Pozueta Romero, Javier
Mitsui, Toshiaki
Itoh, Kimiko
spellingShingle Fukushima, Mamiko
Almagro, Goizeder
Hanashiro, Isao
Pozueta Romero, Javier
Mitsui, Toshiaki
Itoh, Kimiko
Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University
author_sort Fukushima, Mamiko
title Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University
title_short Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University
title_full Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University
title_fullStr Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University
title_full_unstemmed Production and characterization of novel glycogen-based polymer. Joint research by Public University of Navarra and Niigata University
title_sort production and characterization of novel glycogen-based polymer. joint research by public university of navarra and niigata university
publishDate 2015-09
url http://hdl.handle.net/10261/142378
http://dx.doi.org/10.13039/501100001691
work_keys_str_mv AT fukushimamamiko productionandcharacterizationofnovelglycogenbasedpolymerjointresearchbypublicuniversityofnavarraandniigatauniversity
AT almagrogoizeder productionandcharacterizationofnovelglycogenbasedpolymerjointresearchbypublicuniversityofnavarraandniigatauniversity
AT hanashiroisao productionandcharacterizationofnovelglycogenbasedpolymerjointresearchbypublicuniversityofnavarraandniigatauniversity
AT pozuetaromerojavier productionandcharacterizationofnovelglycogenbasedpolymerjointresearchbypublicuniversityofnavarraandniigatauniversity
AT mitsuitoshiaki productionandcharacterizationofnovelglycogenbasedpolymerjointresearchbypublicuniversityofnavarraandniigatauniversity
AT itohkimiko productionandcharacterizationofnovelglycogenbasedpolymerjointresearchbypublicuniversityofnavarraandniigatauniversity
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