Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae)
Entomopathogenic nematodes (EPN) are excellent biocontrol agents against various insect pests. Novel biotechnological approaches can enhance their utility against insects above-ground, opening a new venue for selecting superior EPN against certain insects. We hypothesize that different populations of the same species but from different origins (habitat, ecoregion) will differ in their virulence. This study aimed to evaluate the virulence of various EPN populations against two pests of worldwide incidence and damage to high value crops: Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae). We tested 10 EPN populations belonging to three EPN species: Heterorhabditis bacteriophora (Koppert, MG-618b, AM-203, RM-102), Steinernema feltiae (Koppert, RS-5, AM-25, RM-107), and Steinernema carpocapsae (Koppert, MG-596a). Each EPN population was tested at two concentrations. Frankliniella occidentalis was tested at 160 and 80 IJs/cm2 and T. absoluta at 21 and 4 IJs/cm2. Control treatments followed the same experimental procedure but only adding distilled water. Overall, whenever different, higher IJs concentration resulted in lower adult emergence, higher larval mortality, and shorter time to kill the insects. Considering the low concentration, S. feltiae provided the best results for both insects and instars investigated, while H. bacteriophora and S. carpocapsae required a high concentration to reach similar or slightly better results. Differences among populations of each of the species were detected, but only the native populations of H. bacteriophora populations showed consistently higher control values against both insects/instar compared with the commercial one. Differences among S. feltiae and S. carpocapsae populations depended on the IJs concentration, insect, and instar. We consider S. feltiae a very promising species for their application against F. occidentalis and T. absoluta, with the Koppert population as the most consistent among the populations tested. Specific EPN-populations of S. carpocapsae and H. bacteriophora were good candidates against certain instar/insects at high concentrations. This study emphasized the importance of intraspecific variability for EPN virulence.
Main Authors: | , , , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Published: |
Society of Nematologists
2021-12-14
|
Subjects: | Heterorhabditis, Steinernema, Tomato, Aerial insect-pests, |
Online Access: | http://hdl.handle.net/10261/262566 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003329 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-icvv-es-10261-262566 |
---|---|
record_format |
koha |
institution |
ICVV ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-icvv-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del ICVV España |
topic |
Heterorhabditis Steinernema Tomato Aerial insect-pests Heterorhabditis Steinernema Tomato Aerial insect-pests |
spellingShingle |
Heterorhabditis Steinernema Tomato Aerial insect-pests Heterorhabditis Steinernema Tomato Aerial insect-pests Campos-Herrera, R. Vicente-Díez, Ignacio Galeano, Magda Chelkha, Maryam González-Trujillo, M. Puelles, Miguel Labarga, David Pou, Alicia Calvo-Garrido, Javier Belda, J. E. Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae) |
description |
Entomopathogenic nematodes (EPN) are excellent biocontrol agents against various insect pests. Novel biotechnological approaches can enhance their utility against insects above-ground, opening a new venue for selecting superior EPN against certain insects. We hypothesize that different populations of the same species but from different origins (habitat, ecoregion) will differ in their virulence. This study aimed to evaluate the virulence of various EPN populations against two pests of worldwide incidence and damage to high value crops: Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae). We tested 10 EPN populations belonging to three EPN species: Heterorhabditis bacteriophora (Koppert, MG-618b, AM-203, RM-102), Steinernema feltiae (Koppert, RS-5, AM-25, RM-107), and Steinernema carpocapsae (Koppert, MG-596a). Each EPN population was tested at two concentrations. Frankliniella occidentalis was tested at 160 and 80 IJs/cm2 and T. absoluta at 21 and 4 IJs/cm2. Control treatments followed the same experimental procedure but only adding distilled water. Overall, whenever different, higher IJs concentration resulted in lower adult emergence, higher larval mortality, and shorter time to kill the insects. Considering the low concentration, S. feltiae provided the best results for both insects and instars investigated, while H. bacteriophora and S. carpocapsae required a high concentration to reach similar or slightly better results. Differences among populations of each of the species were detected, but only the native populations of H. bacteriophora populations showed consistently higher control values against both insects/instar compared with the commercial one. Differences among S. feltiae and S. carpocapsae populations depended on the IJs concentration, insect, and instar. We consider S. feltiae a very promising species for their application against F. occidentalis and T. absoluta, with the Koppert population as the most consistent among the populations tested. Specific EPN-populations of S. carpocapsae and H. bacteriophora were good candidates against certain instar/insects at high concentrations. This study emphasized the importance of intraspecific variability for EPN virulence. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Campos-Herrera, R. Vicente-Díez, Ignacio Galeano, Magda Chelkha, Maryam González-Trujillo, M. Puelles, Miguel Labarga, David Pou, Alicia Calvo-Garrido, Javier Belda, J. E. |
format |
artículo |
topic_facet |
Heterorhabditis Steinernema Tomato Aerial insect-pests |
author |
Campos-Herrera, R. Vicente-Díez, Ignacio Galeano, Magda Chelkha, Maryam González-Trujillo, M. Puelles, Miguel Labarga, David Pou, Alicia Calvo-Garrido, Javier Belda, J. E. |
author_sort |
Campos-Herrera, R. |
title |
Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae) |
title_short |
Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae) |
title_full |
Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae) |
title_fullStr |
Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae) |
title_full_unstemmed |
Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae) |
title_sort |
intraspecific virulence of entomopathogenic nematodes against the pests frankliniella occidentalis (thysanoptera: thripidae) and tuta absoluta (lepidoptera: gelechiidae) |
publisher |
Society of Nematologists |
publishDate |
2021-12-14 |
url |
http://hdl.handle.net/10261/262566 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003329 |
work_keys_str_mv |
AT camposherrerar intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT vicentediezignacio intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT galeanomagda intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT chelkhamaryam intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT gonzaleztrujillom intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT puellesmiguel intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT labargadavid intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT poualicia intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT calvogarridojavier intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae AT beldaje intraspecificvirulenceofentomopathogenicnematodesagainstthepestsfrankliniellaoccidentalisthysanopterathripidaeandtutaabsolutalepidopteragelechiidae |
_version_ |
1777671026026479616 |
spelling |
dig-icvv-es-10261-2625662022-03-16T07:29:23Z Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae) Campos-Herrera, R. Vicente-Díez, Ignacio Galeano, Magda Chelkha, Maryam González-Trujillo, M. Puelles, Miguel Labarga, David Pou, Alicia Calvo-Garrido, Javier Belda, J. E. Ministerio de Economía y Competitividad (España) Universidad de La Rioja Ministry of National Education, Vocational Training, Higher Education and Scientific Research (Morocco) Consejo Superior de Investigaciones Científicas (España) Gobierno de La Rioja Heterorhabditis Steinernema Tomato Aerial insect-pests Entomopathogenic nematodes (EPN) are excellent biocontrol agents against various insect pests. Novel biotechnological approaches can enhance their utility against insects above-ground, opening a new venue for selecting superior EPN against certain insects. We hypothesize that different populations of the same species but from different origins (habitat, ecoregion) will differ in their virulence. This study aimed to evaluate the virulence of various EPN populations against two pests of worldwide incidence and damage to high value crops: Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae). We tested 10 EPN populations belonging to three EPN species: Heterorhabditis bacteriophora (Koppert, MG-618b, AM-203, RM-102), Steinernema feltiae (Koppert, RS-5, AM-25, RM-107), and Steinernema carpocapsae (Koppert, MG-596a). Each EPN population was tested at two concentrations. Frankliniella occidentalis was tested at 160 and 80 IJs/cm2 and T. absoluta at 21 and 4 IJs/cm2. Control treatments followed the same experimental procedure but only adding distilled water. Overall, whenever different, higher IJs concentration resulted in lower adult emergence, higher larval mortality, and shorter time to kill the insects. Considering the low concentration, S. feltiae provided the best results for both insects and instars investigated, while H. bacteriophora and S. carpocapsae required a high concentration to reach similar or slightly better results. Differences among populations of each of the species were detected, but only the native populations of H. bacteriophora populations showed consistently higher control values against both insects/instar compared with the commercial one. Differences among S. feltiae and S. carpocapsae populations depended on the IJs concentration, insect, and instar. We consider S. feltiae a very promising species for their application against F. occidentalis and T. absoluta, with the Koppert population as the most consistent among the populations tested. Specific EPN-populations of S. carpocapsae and H. bacteriophora were good candidates against certain instar/insects at high concentrations. This study emphasized the importance of intraspecific variability for EPN virulence. RCH is awarded by Ramon y Cajal contract award MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your future”: Grant RYC-2016-19939 from the Government of Spain. IVD is supported with a FPI-UR (2021) fellowship (Universidad de La Rioja, Spain). MC is supported by a Moroccan scholarship for the Ministry of National Education, Vocational Training, Higher Education and Scientific Research, and the travel assistance associated with the grant CSIC I-COOP + 2018 grant (COOPA20231). MMGT is funded by the Program JAE-Intro CSIC call 2020 (JAEINT20_EX_0939). MP and DL are funded by an introduction to research fellowship from Government of La Rioja (CAR 2020). The study was performed under the Research and Innovation grant at CSIC ref. 20200154 and 20194898. Peer reviewed 2022-03-02T12:20:27Z 2022-03-02T12:20:27Z 2021-12-14 2022-03-02T12:20:28Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.21307/jofnem-2021-102 issn: 2640-396X Journal of Nematology 53: e2021-102 (2021) http://hdl.handle.net/10261/262566 10.21307/jofnem-2021-102 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003329 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//RYC-2016-19939 Publisher's version Campos-Herrera,R., Vicente-Díez,I., Galeano,M., Chelkha,M., Chelkha,M., del Mar González-Trujillo,M., Puelles,M., Labarga,D., Pou,A., Calvo,J., & Belda,J.E.(2021).Intraspecific virulence of entomopathogenic nematodes against the pests Frankliniella occidentalis (Thysanoptera: Thripidae) and Tuta absoluta (Lepidoptera: Gelechiidae) [Dataset]; DIGITAL.CSIC, https://doi.org/10.20350/digitalCSIC/14558 http://dx.doi.org/10.21307/jofnem-2021-102 Sí open Society of Nematologists |