Interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine.
Coeliac disease (CD) development involves genetic (HLA-DQ2/DQ8) and environmental factors. Herein, the influence of the HLA-DQ genotype on the gut colonization process of breast-fed children was determined. A cohort of 20 newborns, with at least one first-degree relative with CD, were classified according to their HLA-DQ genotype into high, intermediate and low genetic risk groups, showing 24-28%, 7-8% and less than 1% probability to develop CD, respectively. Faecal microbiota was analysed at 7 days, 1 and 4 months of children's age by fluorescence in situ hybridization. When considering all data, Gram-negative bacteria and Bacteroides-Prevotella group proportions were higher (P<0.05) in the high than in the intermediate and low genetic risk groups. E. coli, Streptococcus-Lactococcus, E. rectale-C. coccoides, sulphate-reducing bacteria, C. lituseburense and C. histolyticum group proportions were also significantly higher (P<0.05) in the high than in the low genetic risk group. Correlations between these bacterial groups and the genetic risk were also detected (P<0.05). In addition, the number and type of CD relative seemed to influence (P<0.050) these bacterial proportions in children at CD risk. At 4 months of age, similar relationships were established between the high genetic risk to develop CD and the proportions of Streptococcus-Lactococcus (P<0.05), E. rectale-C. coccoides (P<0.05), C. lituseburense (P<0.05), C. histolyticum (P<0.05), Bacteroides-Prevotella (P<0.10) groups and total Gram-negative bacteria (P<0.05). The results suggest a relationship between HLA-DQ genes and the gut microbial colonization process that could lead to a change in the way this disorder is investigated.
| Main Authors: | , , , |
|---|---|
| Format: | artículo biblioteca |
| Published: |
Caister Academic Press
2010
|
| Online Access: | http://hdl.handle.net/10261/94237 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-ictan-es-10261-94237 |
|---|---|
| record_format |
koha |
| spelling |
dig-ictan-es-10261-942372021-06-22T12:50:43Z Interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine. Palma, Giada de Nova, Esther Pozo Rubio, Tamara Sanz, Yolanda Coeliac disease (CD) development involves genetic (HLA-DQ2/DQ8) and environmental factors. Herein, the influence of the HLA-DQ genotype on the gut colonization process of breast-fed children was determined. A cohort of 20 newborns, with at least one first-degree relative with CD, were classified according to their HLA-DQ genotype into high, intermediate and low genetic risk groups, showing 24-28%, 7-8% and less than 1% probability to develop CD, respectively. Faecal microbiota was analysed at 7 days, 1 and 4 months of children's age by fluorescence in situ hybridization. When considering all data, Gram-negative bacteria and Bacteroides-Prevotella group proportions were higher (P<0.05) in the high than in the intermediate and low genetic risk groups. E. coli, Streptococcus-Lactococcus, E. rectale-C. coccoides, sulphate-reducing bacteria, C. lituseburense and C. histolyticum group proportions were also significantly higher (P<0.05) in the high than in the low genetic risk group. Correlations between these bacterial groups and the genetic risk were also detected (P<0.05). In addition, the number and type of CD relative seemed to influence (P<0.050) these bacterial proportions in children at CD risk. At 4 months of age, similar relationships were established between the high genetic risk to develop CD and the proportions of Streptococcus-Lactococcus (P<0.05), E. rectale-C. coccoides (P<0.05), C. lituseburense (P<0.05), C. histolyticum (P<0.05), Bacteroides-Prevotella (P<0.10) groups and total Gram-negative bacteria (P<0.05). The results suggest a relationship between HLA-DQ genes and the gut microbial colonization process that could lead to a change in the way this disorder is investigated. Peer Reviewed 2014-03-26T09:35:40Z 2014-03-26T09:35:40Z 2010 2014-03-26T09:35:40Z artículo http://purl.org/coar/resource_type/c_6501 issn: 1467-3045 Current Issues in Molecular Biology 12: 1- 10 (2010) http://hdl.handle.net/10261/94237 open Caister Academic Press |
| institution |
ICTAN ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-ictan-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del ICTAN España |
| description |
Coeliac disease (CD) development involves genetic (HLA-DQ2/DQ8) and environmental factors. Herein, the influence of the HLA-DQ genotype on the gut colonization process of breast-fed children was determined. A cohort of 20 newborns, with at least one first-degree relative with CD, were classified according to their HLA-DQ genotype into high, intermediate and low genetic risk groups, showing 24-28%, 7-8% and less than 1% probability to develop CD, respectively. Faecal microbiota was analysed at 7 days, 1 and 4 months of children's age by fluorescence in situ hybridization. When considering all data, Gram-negative bacteria and Bacteroides-Prevotella group proportions were higher (P<0.05) in the high than in the intermediate and low genetic risk groups. E. coli, Streptococcus-Lactococcus, E. rectale-C. coccoides, sulphate-reducing bacteria, C. lituseburense and C. histolyticum group proportions were also significantly higher (P<0.05) in the high than in the low genetic risk group. Correlations between these bacterial groups and the genetic risk were also detected (P<0.05). In addition, the number and type of CD relative seemed to influence (P<0.050) these bacterial proportions in children at CD risk. At 4 months of age, similar relationships were established between the high genetic risk to develop CD and the proportions of Streptococcus-Lactococcus (P<0.05), E. rectale-C. coccoides (P<0.05), C. lituseburense (P<0.05), C. histolyticum (P<0.05), Bacteroides-Prevotella (P<0.10) groups and total Gram-negative bacteria (P<0.05). The results suggest a relationship between HLA-DQ genes and the gut microbial colonization process that could lead to a change in the way this disorder is investigated. |
| format |
artículo |
| author |
Palma, Giada de Nova, Esther Pozo Rubio, Tamara Sanz, Yolanda |
| spellingShingle |
Palma, Giada de Nova, Esther Pozo Rubio, Tamara Sanz, Yolanda Interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine. |
| author_facet |
Palma, Giada de Nova, Esther Pozo Rubio, Tamara Sanz, Yolanda |
| author_sort |
Palma, Giada de |
| title |
Interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine. |
| title_short |
Interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine. |
| title_full |
Interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine. |
| title_fullStr |
Interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine. |
| title_full_unstemmed |
Interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine. |
| title_sort |
interplay between human leukocyte antigen genes and the microbial colonization process of the newborn intestine. |
| publisher |
Caister Academic Press |
| publishDate |
2010 |
| url |
http://hdl.handle.net/10261/94237 |
| work_keys_str_mv |
AT palmagiadade interplaybetweenhumanleukocyteantigengenesandthemicrobialcolonizationprocessofthenewbornintestine AT novaesther interplaybetweenhumanleukocyteantigengenesandthemicrobialcolonizationprocessofthenewbornintestine AT pozorubiotamara interplaybetweenhumanleukocyteantigengenesandthemicrobialcolonizationprocessofthenewbornintestine AT sanzyolanda interplaybetweenhumanleukocyteantigengenesandthemicrobialcolonizationprocessofthenewbornintestine |
| _version_ |
1777670467785588736 |