Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry
Enantiomeric composition of selected chiral compounds present in complex mixtures is determined by using the online coupling of reversed-phase liquid chromatography (LC) to gas chromatography (GC) and mass spectrometry. Integration of sample preparation into GC analysis, in a completely automated way, is achieved by means of the effective clean-up resulting from both the LC fractionation step and the eluent elimination provided by the through oven transfer adsorption desorption system used for LC-GC interfacing. The possibilities of the technique are illustrated through some examples concerning the stereodifferentiation in essential oils of major and minor chiral compounds via LC-GC transfer of different volume fractions, ranging from 0.5 to 1.9 ml, which show the significance of the window size for the determination of enantiomeric profiles. © 2012 Wiley Periodicals, Inc.
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Format: | artículo biblioteca |
Language: | English |
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John Wiley & Sons
2012
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Subjects: | Enantiomeric resolution, Chirality, Multidimensional chromatography, Online coupled RPLC–GC–MS; TOTAD interface, |
Online Access: | http://hdl.handle.net/10261/58109 |
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dig-ictan-es-10261-581092016-12-21T08:45:44Z Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry Barba, Carmen Martínez, Rosa M. Calvo, Marta Santa-María, Guillermo Herraiz Carasa, Marta Enantiomeric resolution Chirality Multidimensional chromatography Online coupled RPLC–GC–MS; TOTAD interface Enantiomeric composition of selected chiral compounds present in complex mixtures is determined by using the online coupling of reversed-phase liquid chromatography (LC) to gas chromatography (GC) and mass spectrometry. Integration of sample preparation into GC analysis, in a completely automated way, is achieved by means of the effective clean-up resulting from both the LC fractionation step and the eluent elimination provided by the through oven transfer adsorption desorption system used for LC-GC interfacing. The possibilities of the technique are illustrated through some examples concerning the stereodifferentiation in essential oils of major and minor chiral compounds via LC-GC transfer of different volume fractions, ranging from 0.5 to 1.9 ml, which show the significance of the window size for the determination of enantiomeric profiles. © 2012 Wiley Periodicals, Inc. Financial assistance from the Spanish Science and Technology Commission (projects CTQ2006-01687 and CTQ2009-09208) Peer Reviewed 2012-10-16T10:47:45Z 2012-10-16T10:47:45Z 2012 2012-10-16T10:47:45Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1002/chir.22036 issn: 0899-0042 Chirality 24: 420- 426 (2012) http://hdl.handle.net/10261/58109 10.1002/chir.22036 en none John Wiley & Sons |
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Enantiomeric resolution Chirality Multidimensional chromatography Online coupled RPLC–GC–MS; TOTAD interface Enantiomeric resolution Chirality Multidimensional chromatography Online coupled RPLC–GC–MS; TOTAD interface |
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Enantiomeric resolution Chirality Multidimensional chromatography Online coupled RPLC–GC–MS; TOTAD interface Enantiomeric resolution Chirality Multidimensional chromatography Online coupled RPLC–GC–MS; TOTAD interface Barba, Carmen Martínez, Rosa M. Calvo, Marta Santa-María, Guillermo Herraiz Carasa, Marta Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry |
description |
Enantiomeric composition of selected chiral compounds present in complex mixtures is determined by using the online coupling of reversed-phase liquid chromatography (LC) to gas chromatography (GC) and mass spectrometry. Integration of sample preparation into GC analysis, in a completely automated way, is achieved by means of the effective clean-up resulting from both the LC fractionation step and the eluent elimination provided by the through oven transfer adsorption desorption system used for LC-GC interfacing. The possibilities of the technique are illustrated through some examples concerning the stereodifferentiation in essential oils of major and minor chiral compounds via LC-GC transfer of different volume fractions, ranging from 0.5 to 1.9 ml, which show the significance of the window size for the determination of enantiomeric profiles. © 2012 Wiley Periodicals, Inc. |
format |
artículo |
topic_facet |
Enantiomeric resolution Chirality Multidimensional chromatography Online coupled RPLC–GC–MS; TOTAD interface |
author |
Barba, Carmen Martínez, Rosa M. Calvo, Marta Santa-María, Guillermo Herraiz Carasa, Marta |
author_facet |
Barba, Carmen Martínez, Rosa M. Calvo, Marta Santa-María, Guillermo Herraiz Carasa, Marta |
author_sort |
Barba, Carmen |
title |
Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry |
title_short |
Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry |
title_full |
Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry |
title_fullStr |
Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry |
title_full_unstemmed |
Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry |
title_sort |
chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry |
publisher |
John Wiley & Sons |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/58109 |
work_keys_str_mv |
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