Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre

Okara is an abundant and inexpensive by-product from soybean, rich in total dietary fibre (> 55% dry weight), but poor in soluble dietary fibre (SDF, < 5% dw). A combined method of high hydrostatic pressure (HHP) aided by the food grade enzyme Ultraflo® L was used for SDF maximization. At atmospheric pressure, incubation time was not a key factor, and a ratio of 1:40 (enzyme:Okara, v/w) was able to saturate the enzyme within 120-150 min. When HHP plus Ultraflo® L were applied, a synergy between both treatments was observed. Thus, at 600 MPa, 0.025% Ultraflo® L and 30 min treatment, soluble carbohydrate added up to 15.64 ± 0.32%, consisting of two peaks (9.14 ± 0.18 and 0.57 ± 0.01 kDa), determined by HPLC-ELSD. The combination of HHP plus Ultraflo® L on Okara improved the solubility of the dietary fibre, making it more suitable to be used in functional foods.

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Bibliographic Details
Main Authors: Pérez-López, Elena, Mateos-Aparicio, Inmaculada, Rupérez Antón, Pilar
Other Authors: Eusko Jaurlaritza
Format: artículo biblioteca
Published: Elsevier 2016
Subjects:Dietary fibre, Agrofood by-product, High hydrostatic pressure, Glucanase, Soybean, Okara,
Online Access:http://hdl.handle.net/10261/171586
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003086
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spelling dig-ictan-es-10261-1715862020-12-10T15:57:12Z Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar Eusko Jaurlaritza Ministerio de Economía y Competitividad (España) Dietary fibre Agrofood by-product High hydrostatic pressure Glucanase Soybean Okara Okara is an abundant and inexpensive by-product from soybean, rich in total dietary fibre (> 55% dry weight), but poor in soluble dietary fibre (SDF, < 5% dw). A combined method of high hydrostatic pressure (HHP) aided by the food grade enzyme Ultraflo® L was used for SDF maximization. At atmospheric pressure, incubation time was not a key factor, and a ratio of 1:40 (enzyme:Okara, v/w) was able to saturate the enzyme within 120-150 min. When HHP plus Ultraflo® L were applied, a synergy between both treatments was observed. Thus, at 600 MPa, 0.025% Ultraflo® L and 30 min treatment, soluble carbohydrate added up to 15.64 ± 0.32%, consisting of two peaks (9.14 ± 0.18 and 0.57 ± 0.01 kDa), determined by HPLC-ELSD. The combination of HHP plus Ultraflo® L on Okara improved the solubility of the dietary fibre, making it more suitable to be used in functional foods. Elena Pérez-López acknowledges the predoctoral training programme of the Education, Language Policy and Culture of the Basque Government (Spain) (Grant No. PRE_2013_1_682 MOD: B) for financial support. This work was funded by Project AGL2015-69492-R from the Spanish MINECO. Peer Reviewed 2018-10-25T10:52:27Z 2018-10-25T10:52:27Z 2016 2018-10-25T10:52:27Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.ifset.2015.12.017 issn: 1466-8564 e-issn: 1878-5522 Innovative Food Science and Emerging Technologies 33: 32-37 (2016) http://hdl.handle.net/10261/171586 10.1016/j.ifset.2015.12.017 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003086 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-69492-R Sí none Elsevier
institution ICTAN ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ictan-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICTAN España
topic Dietary fibre
Agrofood by-product
High hydrostatic pressure
Glucanase
Soybean
Okara
Dietary fibre
Agrofood by-product
High hydrostatic pressure
Glucanase
Soybean
Okara
spellingShingle Dietary fibre
Agrofood by-product
High hydrostatic pressure
Glucanase
Soybean
Okara
Dietary fibre
Agrofood by-product
High hydrostatic pressure
Glucanase
Soybean
Okara
Pérez-López, Elena
Mateos-Aparicio, Inmaculada
Rupérez Antón, Pilar
Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre
description Okara is an abundant and inexpensive by-product from soybean, rich in total dietary fibre (> 55% dry weight), but poor in soluble dietary fibre (SDF, < 5% dw). A combined method of high hydrostatic pressure (HHP) aided by the food grade enzyme Ultraflo® L was used for SDF maximization. At atmospheric pressure, incubation time was not a key factor, and a ratio of 1:40 (enzyme:Okara, v/w) was able to saturate the enzyme within 120-150 min. When HHP plus Ultraflo® L were applied, a synergy between both treatments was observed. Thus, at 600 MPa, 0.025% Ultraflo® L and 30 min treatment, soluble carbohydrate added up to 15.64 ± 0.32%, consisting of two peaks (9.14 ± 0.18 and 0.57 ± 0.01 kDa), determined by HPLC-ELSD. The combination of HHP plus Ultraflo® L on Okara improved the solubility of the dietary fibre, making it more suitable to be used in functional foods.
author2 Eusko Jaurlaritza
author_facet Eusko Jaurlaritza
Pérez-López, Elena
Mateos-Aparicio, Inmaculada
Rupérez Antón, Pilar
format artículo
topic_facet Dietary fibre
Agrofood by-product
High hydrostatic pressure
Glucanase
Soybean
Okara
author Pérez-López, Elena
Mateos-Aparicio, Inmaculada
Rupérez Antón, Pilar
author_sort Pérez-López, Elena
title Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre
title_short Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre
title_full Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre
title_fullStr Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre
title_full_unstemmed Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre
title_sort okara treated with high hydrostatic pressure assisted by ultraflo® l: effect on solubility of dietary fibre
publisher Elsevier
publishDate 2016
url http://hdl.handle.net/10261/171586
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003086
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