Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre
Okara is an abundant and inexpensive by-product from soybean, rich in total dietary fibre (> 55% dry weight), but poor in soluble dietary fibre (SDF, < 5% dw). A combined method of high hydrostatic pressure (HHP) aided by the food grade enzyme Ultraflo® L was used for SDF maximization. At atmospheric pressure, incubation time was not a key factor, and a ratio of 1:40 (enzyme:Okara, v/w) was able to saturate the enzyme within 120-150 min. When HHP plus Ultraflo® L were applied, a synergy between both treatments was observed. Thus, at 600 MPa, 0.025% Ultraflo® L and 30 min treatment, soluble carbohydrate added up to 15.64 ± 0.32%, consisting of two peaks (9.14 ± 0.18 and 0.57 ± 0.01 kDa), determined by HPLC-ELSD. The combination of HHP plus Ultraflo® L on Okara improved the solubility of the dietary fibre, making it more suitable to be used in functional foods.
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Elsevier
2016
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Subjects: | Dietary fibre, Agrofood by-product, High hydrostatic pressure, Glucanase, Soybean, Okara, |
Online Access: | http://hdl.handle.net/10261/171586 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003086 |
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dig-ictan-es-10261-1715862020-12-10T15:57:12Z Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar Eusko Jaurlaritza Ministerio de Economía y Competitividad (España) Dietary fibre Agrofood by-product High hydrostatic pressure Glucanase Soybean Okara Okara is an abundant and inexpensive by-product from soybean, rich in total dietary fibre (> 55% dry weight), but poor in soluble dietary fibre (SDF, < 5% dw). A combined method of high hydrostatic pressure (HHP) aided by the food grade enzyme Ultraflo® L was used for SDF maximization. At atmospheric pressure, incubation time was not a key factor, and a ratio of 1:40 (enzyme:Okara, v/w) was able to saturate the enzyme within 120-150 min. When HHP plus Ultraflo® L were applied, a synergy between both treatments was observed. Thus, at 600 MPa, 0.025% Ultraflo® L and 30 min treatment, soluble carbohydrate added up to 15.64 ± 0.32%, consisting of two peaks (9.14 ± 0.18 and 0.57 ± 0.01 kDa), determined by HPLC-ELSD. The combination of HHP plus Ultraflo® L on Okara improved the solubility of the dietary fibre, making it more suitable to be used in functional foods. Elena Pérez-López acknowledges the predoctoral training programme of the Education, Language Policy and Culture of the Basque Government (Spain) (Grant No. PRE_2013_1_682 MOD: B) for financial support. This work was funded by Project AGL2015-69492-R from the Spanish MINECO. Peer Reviewed 2018-10-25T10:52:27Z 2018-10-25T10:52:27Z 2016 2018-10-25T10:52:27Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.ifset.2015.12.017 issn: 1466-8564 e-issn: 1878-5522 Innovative Food Science and Emerging Technologies 33: 32-37 (2016) http://hdl.handle.net/10261/171586 10.1016/j.ifset.2015.12.017 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003086 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-69492-R Sí none Elsevier |
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Dietary fibre Agrofood by-product High hydrostatic pressure Glucanase Soybean Okara Dietary fibre Agrofood by-product High hydrostatic pressure Glucanase Soybean Okara |
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Dietary fibre Agrofood by-product High hydrostatic pressure Glucanase Soybean Okara Dietary fibre Agrofood by-product High hydrostatic pressure Glucanase Soybean Okara Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre |
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Okara is an abundant and inexpensive by-product from soybean, rich in total dietary fibre (> 55% dry weight), but poor in soluble dietary fibre (SDF, < 5% dw). A combined method of high hydrostatic pressure (HHP) aided by the food grade enzyme Ultraflo® L was used for SDF maximization. At atmospheric pressure, incubation time was not a key factor, and a ratio of 1:40 (enzyme:Okara, v/w) was able to saturate the enzyme within 120-150 min. When HHP plus Ultraflo® L were applied, a synergy between both treatments was observed. Thus, at 600 MPa, 0.025% Ultraflo® L and 30 min treatment, soluble carbohydrate added up to 15.64 ± 0.32%, consisting of two peaks (9.14 ± 0.18 and 0.57 ± 0.01 kDa), determined by HPLC-ELSD. The combination of HHP plus Ultraflo® L on Okara improved the solubility of the dietary fibre, making it more suitable to be used in functional foods. |
author2 |
Eusko Jaurlaritza |
author_facet |
Eusko Jaurlaritza Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar |
format |
artículo |
topic_facet |
Dietary fibre Agrofood by-product High hydrostatic pressure Glucanase Soybean Okara |
author |
Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar |
author_sort |
Pérez-López, Elena |
title |
Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre |
title_short |
Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre |
title_full |
Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre |
title_fullStr |
Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre |
title_full_unstemmed |
Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre |
title_sort |
okara treated with high hydrostatic pressure assisted by ultraflo® l: effect on solubility of dietary fibre |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/10261/171586 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003086 |
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