Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus

Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus

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22 pages, 3 figures, 3 tables

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Main Authors: Koenig, Samuel, Porte Visa, Cinta, Solé, Montserrat, Sturve, Joachim
Format: artículo biblioteca
Language:English
Published: American Chemical Society 2013-02
Subjects:Gene expression, Biomarkers, Vitellogenin, Alkylphenols, Polycyclic aromatic hydrocarbons, Bile metabolites, Deep-sea fish, Mediterranean, PAH, Vtg, AP,
Online Access:http://hdl.handle.net/10261/74912
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spelling dig-icm-es-10261-749122020-12-01T10:07:53Z Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus Koenig, Samuel Porte Visa, Cinta Solé, Montserrat Sturve, Joachim Gene expression Biomarkers Vitellogenin Alkylphenols Polycyclic aromatic hydrocarbons Bile metabolites Deep-sea fish Mediterranean PAH Vtg AP 22 pages, 3 figures, 3 tables Biliary polycyclic aromatic hydrocarbon (PAH) and alkylphenol (AP) metabolites, hepatic gene expression, and corresponding enzyme activities were determined in the deep-sea fish Alepocephalus rostratus from two sites within the Mediterranean. Biliary metabolites included the hydroxylated PAH metabolites (OH-PAHs) 1-naphthol, 2-naphthol, 9-fluorenol, 9-phenanthrol, and 1-pyrenol and the APs 4-nonylphenol (NP) and 4-tert-octylphenol (OP). Five biomarker genes, namely, cytochrome P450 1A (CYP1A), vitellogenin (Vtg), catalase (CAT), Cu/Zn-superoxide-dismutase (Cu/Zn-SOD), and glutathione reductase (GR), were quantified using qRT-PCR. Moreover, corresponding enzyme activities (ethoxyresorufin-O-deethylase (EROD), CAT, SOD, and GR) were also determined. The ΣOH-PAHs detected ranged from 21.1 to 300.3 ng/g bile and were mainly composed of 1-naphthol. Both NP and OP metabolites were detected in all samples with concentrations ranging from 17.4 to 107.2 ng/g bile and 4.9 to 17.3 ng/g bile, respectively, and levels were significantly higher in samples from the western Mediterranean (WM) compared to those from the Catalan slope (CS). Accordingly, gene expression was significantly induced in male fish from the WM; however, these results were not reflected in enzyme activity levels. In particular, males caught at 2000 m in the WM exhibited 35-times higher Vtg levels compared to those from the CS, suggesting that endocrine-disrupting effects may potentially be occurring in such remote environments as the deep-sea The present study was funded by the Spanish Science and Technology Ministry projects PROMETEO (CTM2007-66316-C02-02/MAR), BIOFUN (CTM2007-28739-E/MAR) and the HERMIONE project (EC-FP7 contract number 226354). Samuel Koenig holds a PhD grant (AFR 08/067) from the Fonds National de la Recherche (FNR), Luxembourg. The authors wish to thank the DeepMed Research Group (ICM-CSIC) and the R/V Garcia del Cid (CSIC) and R/V Sarmiento de Gamboa (CSIC) crews for helping with sampling. Furthermore, the authors acknowledge the help by R. Chaler, D. Fanjul, M. Comesaña with GC-MS instrumental analysis, as well as the technical assistance by N. Asker and M. Hultman with qPCR analysis Peer reviewed 2013-04-23T10:32:07Z 2013-04-23T10:32:07Z 2013-02 artículo http://purl.org/coar/resource_type/c_6501 Environmental Science and Technology - Columbus 47(6): 2854-2861 (2013) 0013-936X http://hdl.handle.net/10261/74912 10.1021/es304345s 1520-5851 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/226354 https://doi.org/10.1021/es304345s open American Chemical Society
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Gene expression
Biomarkers
Vitellogenin
Alkylphenols
Polycyclic aromatic hydrocarbons
Bile metabolites
Deep-sea fish
Mediterranean
PAH
Vtg
AP
Gene expression
Biomarkers
Vitellogenin
Alkylphenols
Polycyclic aromatic hydrocarbons
Bile metabolites
Deep-sea fish
Mediterranean
PAH
Vtg
AP
spellingShingle Gene expression
Biomarkers
Vitellogenin
Alkylphenols
Polycyclic aromatic hydrocarbons
Bile metabolites
Deep-sea fish
Mediterranean
PAH
Vtg
AP
Gene expression
Biomarkers
Vitellogenin
Alkylphenols
Polycyclic aromatic hydrocarbons
Bile metabolites
Deep-sea fish
Mediterranean
PAH
Vtg
AP
Koenig, Samuel
Porte Visa, Cinta
Solé, Montserrat
Sturve, Joachim
Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus
description 22 pages, 3 figures, 3 tables
format artículo
topic_facet Gene expression
Biomarkers
Vitellogenin
Alkylphenols
Polycyclic aromatic hydrocarbons
Bile metabolites
Deep-sea fish
Mediterranean
PAH
Vtg
AP
author Koenig, Samuel
Porte Visa, Cinta
Solé, Montserrat
Sturve, Joachim
author_facet Koenig, Samuel
Porte Visa, Cinta
Solé, Montserrat
Sturve, Joachim
author_sort Koenig, Samuel
title Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus
title_short Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus
title_full Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus
title_fullStr Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus
title_full_unstemmed Biliary PAH and Alkylphenol Metabolites, Biomarker Enzyme Activities, and Gene Expression Levels in the Deep-Sea Fish Alepocephalus rostratus
title_sort biliary pah and alkylphenol metabolites, biomarker enzyme activities, and gene expression levels in the deep-sea fish alepocephalus rostratus
publisher American Chemical Society
publishDate 2013-02
url http://hdl.handle.net/10261/74912
work_keys_str_mv AT koenigsamuel biliarypahandalkylphenolmetabolitesbiomarkerenzymeactivitiesandgeneexpressionlevelsinthedeepseafishalepocephalusrostratus
AT portevisacinta biliarypahandalkylphenolmetabolitesbiomarkerenzymeactivitiesandgeneexpressionlevelsinthedeepseafishalepocephalusrostratus
AT solemontserrat biliarypahandalkylphenolmetabolitesbiomarkerenzymeactivitiesandgeneexpressionlevelsinthedeepseafishalepocephalusrostratus
AT sturvejoachim biliarypahandalkylphenolmetabolitesbiomarkerenzymeactivitiesandgeneexpressionlevelsinthedeepseafishalepocephalusrostratus
_version_ 1777665900904710144

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