Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle

Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle

8 pages, 8 figures, 1 table

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Bibliographic Details
Main Authors: Mechaly, Alejandro S., Viñas, Jordi, Piferrer, Francesc
Format: artículo biblioteca
Language:English
Published: Elsevier 2012-08
Subjects:Kiss2, Kiss2r, gpr54, fshβ, lhβ, Puberty, Reproduction, Fish,
Online Access:http://hdl.handle.net/10261/57255
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spelling dig-icm-es-10261-572552020-11-27T18:47:10Z Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle Mechaly, Alejandro S. Viñas, Jordi Piferrer, Francesc Kiss2 Kiss2r gpr54 fshβ lhβ Puberty Reproduction Fish 8 pages, 8 figures, 1 table Kisspeptin is thought to have a major role in the control of the onset of puberty in vertebrates. However, our current understanding of its function in fish and how it integrates with other hormones is incomplete due to the high diversity of this group of animals and a still limited amount of available data. This study examined the temporal and spatial changes in expression of kisspeptin, gonadotropins and their respective receptors in the Senegalese sole during a full reproductive cycle. Kiss2 and kiss2r expression was determined by qRT-PCR in the forebrain and midbrain while expression of fshβ and lhβ was determined in the pituitary and fshr and lhr in the gonads. Plasma levels of testosterone (T), 11-ketotestosterone (11-KT) and estradiol-17β were measured by ELISA and gonadal maturation was assessed histologically. In males, kiss2 and kiss2r expression in the brain areas examined was highest towards the end of winter, just before the spawning season, which took place the following spring. This coincided with maximum levels of pituitary fshβ and lhβ, plasma T and 11-KT and the highest number of maturing fish. However, these associations were not evident in females, since the highest expression of kiss2, kiss2r and gonadotropins were observed in the fall, winter or spring, depending upon the variable and tissue considered. Taken together, these data show not only temporal and spatial, but also sex-specific differences in the expression of kisspeptin and its receptor. Thus, while expression of kiss2 in Senegalese sole males agrees with what one would expect according to its proposed role as a major regulator of the onset of reproduction, in females the situation was not so clear, since kiss2 and kiss2r expression was highest either before or during the spawning season A.S.M. was supported by a predoctoral scholarship from the Spanish Ministry of Science and Innovation (MICINN). This study was carried out with the financial help of project “Pleurogene”, and also partially funded by project “Aquagenomics” (CDS-2007-0002), both to F.P. Peer reviewed 2012-10-04T07:42:07Z 2012-10-04T07:42:07Z 2012-08 artículo http://purl.org/coar/resource_type/c_6501 Comparative Biochemistry and Physiology - A - Molecular and Integrative Physiology 162(4): 364-371 (2012) 1095-6433 http://hdl.handle.net/10261/57255 10.1016/j.cbpa.2012.04.003 en https://doi.org/10.1016/j.cbpa.2012.04.003 open Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Kiss2
Kiss2r
gpr54
fshβ
lhβ
Puberty
Reproduction
Fish
Kiss2
Kiss2r
gpr54
fshβ
lhβ
Puberty
Reproduction
Fish
spellingShingle Kiss2
Kiss2r
gpr54
fshβ
lhβ
Puberty
Reproduction
Fish
Kiss2
Kiss2r
gpr54
fshβ
lhβ
Puberty
Reproduction
Fish
Mechaly, Alejandro S.
Viñas, Jordi
Piferrer, Francesc
Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle
description 8 pages, 8 figures, 1 table
format artículo
topic_facet Kiss2
Kiss2r
gpr54
fshβ
lhβ
Puberty
Reproduction
Fish
author Mechaly, Alejandro S.
Viñas, Jordi
Piferrer, Francesc
author_facet Mechaly, Alejandro S.
Viñas, Jordi
Piferrer, Francesc
author_sort Mechaly, Alejandro S.
title Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle
title_short Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle
title_full Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle
title_fullStr Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle
title_full_unstemmed Sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the Senegalese sole (Solea senegalensis) during a full reproductive cycle
title_sort sex-specific changes in the expression of kisspeptin, kisspeptin receptor, gonadotropins and gonadotropin receptors in the senegalese sole (solea senegalensis) during a full reproductive cycle
publisher Elsevier
publishDate 2012-08
url http://hdl.handle.net/10261/57255
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AT vinasjordi sexspecificchangesintheexpressionofkisspeptinkisspeptinreceptorgonadotropinsandgonadotropinreceptorsinthesenegalesesolesoleasenegalensisduringafullreproductivecycle
AT piferrerfrancesc sexspecificchangesintheexpressionofkisspeptinkisspeptinreceptorgonadotropinsandgonadotropinreceptorsinthesenegalesesolesoleasenegalensisduringafullreproductivecycle
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