Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4)

Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4)

14 pages, 6 figures, 2 tables

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Main Authors: Rodríguez-Martínez, Raquel, Rocap, Gabrielle, Logares, Ramiro, Romac, Sarah, Massana, Ramon
Format: artículo biblioteca
Language:English
Published: Oxford University Press 2012-05
Subjects:MAST-4, Low evolutionary diversification, Uncultured protist, Pyrosequencing, ITS secondary structure, ITS, MAST,
Online Access:http://hdl.handle.net/10261/56541
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spelling dig-icm-es-10261-565412020-11-03T10:57:29Z Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4) Rodríguez-Martínez, Raquel Rocap, Gabrielle Logares, Ramiro Romac, Sarah Massana, Ramon MAST-4 Low evolutionary diversification Uncultured protist Pyrosequencing ITS secondary structure ITS MAST 14 pages, 6 figures, 2 tables Recent culture-independent studies of marine planktonic protists have unveiled a large diversity at all phylogenetic scales and the existence of novel groups. MAST-4 represents one of these novel uncultured lineages, and it is composed of small (∼2 μm) bacterivorous eukaryotes that are widely distributed in marine systems. MAST-4 accounts for a significant fraction of the marine heterotrophic flagellates at the global level, playing key roles in the marine ecological network. In this study, we investigated the diversity of MAST-4, aiming to assess its limits and structure. Using ribosomal DNA (rDNA) sequences obtained in this study (both pyrosequencing reads and clones with large rDNA operon coverage), complemented with GenBank sequences, we show that MAST-4 is composed of only five main clades, which are well supported by small subunit and large subunit phylogenies. The differences in the conserved regions of the internal transcribed spacers 1 and 2 (ITS1 and ITS2) secondary structures strongly suggest that these five clades are different biological species. Based on intraclade divergence, ITS secondary structures and comparisons of ITS1 and ITS2 trees, we did not find evidence of more than one species within clade A, whereas as many as three species might be present within other clades. Overall, the genetic divergence of MAST-4 was surprisingly low for an organism with a global population size estimated to be around 1024, indicating a very low evolutionary diversification within the group Funding has been provided by FLAME (CGL2010-16304, MICINN, Spain) and BioMarKs (2008-6530, ERA-net Biodiversa, EU) projects to R.M., by an FPI fellowship from the Spanish Ministry of Education and Science to R.R.M., by a Marie Curie Intra-European Fellowship grant PIEFGA-2009-235365 to R.L., and by NSF ATOL 0629521 to G.R. Peer reviewed 2012-09-21T10:11:14Z 2012-09-21T10:11:14Z 2012-05 artículo http://purl.org/coar/resource_type/c_6501 Molecular Biology and Evolution 29(5): 1393-1406 (2012) 0737-4038 http://hdl.handle.net/10261/56541 10.1093/molbev/msr303 1537-1719 en https://doi.org/10.1093/molbev/msr303 open Oxford University Press
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic MAST-4
Low evolutionary diversification
Uncultured protist
Pyrosequencing
ITS secondary structure
ITS
MAST
MAST-4
Low evolutionary diversification
Uncultured protist
Pyrosequencing
ITS secondary structure
ITS
MAST
spellingShingle MAST-4
Low evolutionary diversification
Uncultured protist
Pyrosequencing
ITS secondary structure
ITS
MAST
MAST-4
Low evolutionary diversification
Uncultured protist
Pyrosequencing
ITS secondary structure
ITS
MAST
Rodríguez-Martínez, Raquel
Rocap, Gabrielle
Logares, Ramiro
Romac, Sarah
Massana, Ramon
Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4)
description 14 pages, 6 figures, 2 tables
format artículo
topic_facet MAST-4
Low evolutionary diversification
Uncultured protist
Pyrosequencing
ITS secondary structure
ITS
MAST
author Rodríguez-Martínez, Raquel
Rocap, Gabrielle
Logares, Ramiro
Romac, Sarah
Massana, Ramon
author_facet Rodríguez-Martínez, Raquel
Rocap, Gabrielle
Logares, Ramiro
Romac, Sarah
Massana, Ramon
author_sort Rodríguez-Martínez, Raquel
title Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4)
title_short Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4)
title_full Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4)
title_fullStr Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4)
title_full_unstemmed Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4)
title_sort low evolutionary diversification in a widespread and abundant uncultured protist (mast-4)
publisher Oxford University Press
publishDate 2012-05
url http://hdl.handle.net/10261/56541
work_keys_str_mv AT rodriguezmartinezraquel lowevolutionarydiversificationinawidespreadandabundantunculturedprotistmast4
AT rocapgabrielle lowevolutionarydiversificationinawidespreadandabundantunculturedprotistmast4
AT logaresramiro lowevolutionarydiversificationinawidespreadandabundantunculturedprotistmast4
AT romacsarah lowevolutionarydiversificationinawidespreadandabundantunculturedprotistmast4
AT massanaramon lowevolutionarydiversificationinawidespreadandabundantunculturedprotistmast4
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