Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species

Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species

11 pages, 7 figures, 1 table

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Bibliographic Details
Main Authors: Mechaly, Alejandro S., Viñas, Jordi, Piferrer, Francesc
Format: artículo biblioteca
Language:English
Published: Elsevier 2011-06
Subjects:Kiss2, Kisspeptin-2, Puberty, Fasting, Alternative splicing, Solea senegalensis,
Online Access:http://hdl.handle.net/10261/56475
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spelling dig-icm-es-10261-564752020-11-26T07:35:01Z Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species Mechaly, Alejandro S. Viñas, Jordi Piferrer, Francesc Kiss2 Kisspeptin-2 Puberty Fasting Alternative splicing Solea senegalensis 11 pages, 7 figures, 1 table Kisspeptin signaling in the brain is involved in the control of the onset of puberty in vertebrates. In this study, we present novel evidence indicating that kisspeptin may link energy balance and reproduction. For that purpose, we determined the complete gene structure of kisspeptin in a teleost fish, the Senegalese sole (Ss). In contrast to the situation evident in several fish, in this species only Kiss2 was found. Yet, two Ss Kiss2 isoforms generated by alternative splicing through intronic retention were detected: Ss Kiss2_v1, producing the functional protein, and Ss Kiss2_v2, coding for a truncated, non-functional protein. Specific qPCRs showed that the expression of these two isoforms varied differently in brain and gonads throughout maturation. In addition, and in contrast to what has been observed in mammals, fasting increased hypothalamic mRNA levels of Ss Kiss2_v1, which also caused a concomitant rise in pituitary Ss LH and Ss FSH mRNA. Together, these data indicate the impact of the nutritional status on Kiss mRNA expression as a potential regulatory mechanism for the metabolic control of reproduction in non-mammalian species, albeit with some significant differences with respect to the situation described in mammals This study was carried out with the financial help of the Genoma España project “Pleurogene”, and Ministry of Science and Innovation Consolider project “Aquagenomics” (CDS-2007-0002) to F.P. Peer reviewed 2012-09-19T10:52:44Z 2012-09-19T10:52:44Z 2011-06 artículo http://purl.org/coar/resource_type/c_6501 Molecular and Cellular Endocrinology 339(1-2): 14-24 (2011) 0303-7207 http://hdl.handle.net/10261/56475 10.1016/j.mce.2011.03.004 en https://doi.org/10.1016/j.mce.2011.03.004 open Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Kiss2
Kisspeptin-2
Puberty
Fasting
Alternative splicing
Solea senegalensis
Kiss2
Kisspeptin-2
Puberty
Fasting
Alternative splicing
Solea senegalensis
spellingShingle Kiss2
Kisspeptin-2
Puberty
Fasting
Alternative splicing
Solea senegalensis
Kiss2
Kisspeptin-2
Puberty
Fasting
Alternative splicing
Solea senegalensis
Mechaly, Alejandro S.
Viñas, Jordi
Piferrer, Francesc
Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species
description 11 pages, 7 figures, 1 table
format artículo
topic_facet Kiss2
Kisspeptin-2
Puberty
Fasting
Alternative splicing
Solea senegalensis
author Mechaly, Alejandro S.
Viñas, Jordi
Piferrer, Francesc
author_facet Mechaly, Alejandro S.
Viñas, Jordi
Piferrer, Francesc
author_sort Mechaly, Alejandro S.
title Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species
title_short Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species
title_full Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species
title_fullStr Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species
title_full_unstemmed Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): Characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species
title_sort gene structure analysis of kisspeptin-2 (kiss2) in the senegalese sole (solea senegalensis): characterization of two splice variants of kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species
publisher Elsevier
publishDate 2011-06
url http://hdl.handle.net/10261/56475
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