Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies

Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies

8 pages, 3 figures, 2 tables

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Bibliographic Details
Main Authors: Solé, Montserrat, Vega, Sofia, Varó, Inmaculada
Format: artículo biblioteca
Language:English
Published: Elsevier 2012-04
Subjects:Characterization, Cholinesterases, Carboxylesterases, Solea senegalensis, CYPs, Fluorimetric substrates, CYP,
Online Access:http://hdl.handle.net/10261/46795
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spelling dig-icm-es-10261-467952020-11-26T18:46:39Z Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies Solé, Montserrat Vega, Sofia Varó, Inmaculada Characterization Cholinesterases Carboxylesterases Solea senegalensis CYPs Fluorimetric substrates CYP 8 pages, 3 figures, 2 tables In fish, the role that cholinesterases (ChEs) play in tissues other than those implicated in neural activity, as well as the involvement of carboxylesterases (CbEs) and cytochrome P450 isoenzymes (CYPs) in drug metabolism needs investigation. For that, Senegalese sole (Solea senegalensis) specimens were selected for characterization of several type B esterases and hepatic CYPs in order to further use this fish as sentinel. ChEs (acetylcholinesterase (AChE) and pseudocholinesterases (butyrylcholinesterase-BuChE and propionilcholinesterase-PrChE)) and CbEs were measured in brain, plasma, kidney, liver, gonad, muscle and gills. Moreover, seven fluorimetric substrates were selected to study CYP related activities in fish liver. The results showed that AChE was the dominant ChE form in brain whereas pseudocholinesterases were absent in most tissues, as demonstrated by low enzymatic activities using specific substrates and the lack of inhibition by iso-OMPA. Plasma exhibited trace activities of all the esterases assayed and no BuChE activity. CbEs were dominant in liver, but they were also present in kidney and brain. For CbE determination, α-naphtyl acetate (αNA) was seen as the most adequate substrate as it displayed higher enzymatic activities and showed more in vitro sensitivity to the carbamate eserine and the organophosphate pesticide dichlorvos. Alkoxyresorufin-O-dealkylase (EROD and BFCOD) activities, indicative in mammals of CYP1A and CYP3A subfamilies, respectively, were the highest microsomal CYP-related activities in liver. The results of this preliminary work allow us to select the most adequate esterase substrate, tissue and hepatic CYP substrate for further monitoring studies This work was financed by the Ministry Science and Innovation of Spain (refCTM2010-16611) Peer reviewed 2012-03-08T13:41:52Z 2012-03-08T13:41:52Z 2012-04 artículo http://purl.org/coar/resource_type/c_6501 Ecotoxicology and Environmental Safety 78: 72-79 (2012) 0147-6513 http://hdl.handle.net/10261/46795 10.1016/j.ecoenv.2011.11.013 1090-2414 en https://doi.org/10.1016/j.ecoenv.2011.11.013 open Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Characterization
Cholinesterases
Carboxylesterases
Solea senegalensis
CYPs
Fluorimetric substrates
CYP
Characterization
Cholinesterases
Carboxylesterases
Solea senegalensis
CYPs
Fluorimetric substrates
CYP
spellingShingle Characterization
Cholinesterases
Carboxylesterases
Solea senegalensis
CYPs
Fluorimetric substrates
CYP
Characterization
Cholinesterases
Carboxylesterases
Solea senegalensis
CYPs
Fluorimetric substrates
CYP
Solé, Montserrat
Vega, Sofia
Varó, Inmaculada
Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies
description 8 pages, 3 figures, 2 tables
format artículo
topic_facet Characterization
Cholinesterases
Carboxylesterases
Solea senegalensis
CYPs
Fluorimetric substrates
CYP
author Solé, Montserrat
Vega, Sofia
Varó, Inmaculada
author_facet Solé, Montserrat
Vega, Sofia
Varó, Inmaculada
author_sort Solé, Montserrat
title Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies
title_short Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies
title_full Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies
title_fullStr Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies
title_full_unstemmed Characterization of type “B” esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies
title_sort characterization of type “b” esterases and hepatic cyp450 isoenzimes in senegalese sole for their further application in monitoring studies
publisher Elsevier
publishDate 2012-04
url http://hdl.handle.net/10261/46795
work_keys_str_mv AT solemontserrat characterizationoftypebesterasesandhepaticcyp450isoenzimesinsenegalesesolefortheirfurtherapplicationinmonitoringstudies
AT vegasofia characterizationoftypebesterasesandhepaticcyp450isoenzimesinsenegalesesolefortheirfurtherapplicationinmonitoringstudies
AT varoinmaculada characterizationoftypebesterasesandhepaticcyp450isoenzimesinsenegalesesolefortheirfurtherapplicationinmonitoringstudies
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