Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs)

Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs)

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7 pages, 3 figures, 4 tables

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Main Authors: Koenig, Samuel, Fernández Ramón, M. Pilar, Solé, Montserrat
Format: artículo biblioteca
Language:English
Published: Elsevier 2012-02
Subjects:Cytochrome P450, Biotransformations, Polychlorobiphenyl, Bioaccumulation, Fish, Crustacea, CYP, PCB,
Online Access:http://hdl.handle.net/10261/44812
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spelling dig-icm-es-10261-448122023-01-30T08:53:24Z Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs) Koenig, Samuel Fernández Ramón, M. Pilar Solé, Montserrat Cytochrome P450 Biotransformations Polychlorobiphenyl Bioaccumulation Fish Crustacea CYP PCB 7 pages, 3 figures, 4 tables Variations in cytochrome P450 enzyme (CYPs) distribution and function between animal groups could result in differential metabolism and elimination kinetics for certain contaminants. Although a number of studies have suggested that differences in polychlorobiphenyl (PCB) accumulation profiles between crustacea and fish might result from differential CYP patterns, the relationship between PCB bioaccumulation and CYP capacities has not been demonstrated in these organisms. In the present study we investigated the hepatic microsomal catalytic activities in three deep-sea fish species, Alepocephalus rostratus (Alepocephalidae), Coelorinchus mediterraneus (Macrouridae), and Lepidion lepidion (Moridae), and the decapod crustacean Aristeus antennatus (Decapoda), using six fluorescent CYP-mediated substrates, namely ER (7-ethoxyresorufin), PR (7-pentoxyresorufin), BR (7-benzyloxyresorufin), CEC (3-cyano-7-ethoxycoumarin), DBF (dibenzylfluorescein) and BFC (7-benzyloxy-4-trifluoromethylcoumarin). Furthermore, we related the metabolic activities to the accumulation patterns of 41 PCB congeners in the muscle of these organisms. The results indicated a marked difference in the presence and activities of CYP isoforms between fish and the crustacean A. antennatus. Liver microsomes of the three selected fish species were capable of metabolizing all six CYP-mediated substrates and enzymes were identified as primarily belonging to CYP1A and CYP3A subfamilies. In contrast, hepatopancreas microsomes from A. antennatus only showed activity for PR and DBF substrates, generally related to mammalian CYP2-like enzymes. Furthermore, a direct relationship between metabolic activities and PCB accumulation profiles could be established. Results revealed that A. antennatus accumulated significantly higher proportions of PCBs 28, 52, 118, 138, 158 and 169 than fish, which is in accordance with the previously observed lack of CYP1A-like biotransformation capacities. Moreover, A. antennatus exhibited lower levels of PCBs 87, 149, 153, 170, 180, 183, 194 and 206 indicating that this crustacean is able to metabolize congeners considered mammalian CYP2B inducers. Hence, the present findings highlight the role of CYP-mediated metabolism in the congener-specific accumulation of PCBs in aquatic organisms and stress the need to further investigate quantitative and qualitative differences in xenobiotic metabolism among animal groups The present work was funded by the Spanish Science and Technology Ministry projects PROMETEO (CTM2007-66316-C02-02/MAR) and BIOFUN (CTM2007-28739-E/MAR). Samuel Koenig holds a PhD grant (AFR 08/067) from the Fonds National de la Recherche (FNR), Luxembourg. The authors also wish to thank David Huertas (IDAEA-CSIC), the “Deep-Sea Group” (ICM-CSIC) and the R/V Garcia del Cid (CSIC) crew for helping with field sampling Peer reviewed 2012-01-26T13:54:48Z 2012-01-26T13:54:48Z 2012-02 artículo http://purl.org/coar/resource_type/c_6501 Aquatic Toxicology 108: 11-17 (2012) 0166-445X http://hdl.handle.net/10261/44812 10.1016/j.aquatox.2011.10.016 1879-1514 en https://doi.org/10.1016/j.aquatox.2011.10.016 none Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Cytochrome P450
Biotransformations
Polychlorobiphenyl
Bioaccumulation
Fish
Crustacea
CYP
PCB
Cytochrome P450
Biotransformations
Polychlorobiphenyl
Bioaccumulation
Fish
Crustacea
CYP
PCB
spellingShingle Cytochrome P450
Biotransformations
Polychlorobiphenyl
Bioaccumulation
Fish
Crustacea
CYP
PCB
Cytochrome P450
Biotransformations
Polychlorobiphenyl
Bioaccumulation
Fish
Crustacea
CYP
PCB
Koenig, Samuel
Fernández Ramón, M. Pilar
Solé, Montserrat
Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs)
description 7 pages, 3 figures, 4 tables
format artículo
topic_facet Cytochrome P450
Biotransformations
Polychlorobiphenyl
Bioaccumulation
Fish
Crustacea
CYP
PCB
author Koenig, Samuel
Fernández Ramón, M. Pilar
Solé, Montserrat
author_facet Koenig, Samuel
Fernández Ramón, M. Pilar
Solé, Montserrat
author_sort Koenig, Samuel
title Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs)
title_short Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs)
title_full Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs)
title_fullStr Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs)
title_full_unstemmed Differences in cytochrome P450 enzyme activities between fish and crustacea: Relationship with the bioaccumulation patterns of polychlorobiphenyls (PCBs)
title_sort differences in cytochrome p450 enzyme activities between fish and crustacea: relationship with the bioaccumulation patterns of polychlorobiphenyls (pcbs)
publisher Elsevier
publishDate 2012-02
url http://hdl.handle.net/10261/44812
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AT fernandezramonmpilar differencesincytochromep450enzymeactivitiesbetweenfishandcrustacearelationshipwiththebioaccumulationpatternsofpolychlorobiphenylspcbs
AT solemontserrat differencesincytochromep450enzymeactivitiesbetweenfishandcrustacearelationshipwiththebioaccumulationpatternsofpolychlorobiphenylspcbs
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