Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH
Special issue on Oxygen Minimum Zone Microbiology.-- 11 pages, 5 figures, 3 tables, supporting information https://doi.org/10.1111/1462-2920.15504.-- This is the pre-peer reviewed version of the following article: Yaiza M. Castillo, Irene Forn, Sheree Yau, Xosé Anxelu G. Morán, Laura Alonso-Sáez, Néstor Arandia-Gorostidi, Dolors Vaqué, Marta Sebastián; Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH; Environmental Microbiology 23(6): 3009-3019 (2021), which has been published in final form at https://doi.org/10.1111/1462-2920.15504. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
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Society for Applied Microbiology
2021-06
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dig-icm-es-10261-2458072021-07-16T01:13:14Z Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH Castillo, Yaiza Forn, Irene Yau, Sheree Morán, Xosé Anxelu G. Alonso-Sáez, Laura Arandia-Gorostidi, Néstor Vaqué, Dolors Sebastián, Marta Ministerio de Economía y Competitividad (España) Agencia Estatal de Investigación (España) Special issue on Oxygen Minimum Zone Microbiology.-- 11 pages, 5 figures, 3 tables, supporting information https://doi.org/10.1111/1462-2920.15504.-- This is the pre-peer reviewed version of the following article: Yaiza M. Castillo, Irene Forn, Sheree Yau, Xosé Anxelu G. Morán, Laura Alonso-Sáez, Néstor Arandia-Gorostidi, Dolors Vaqué, Marta Sebastián; Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH; Environmental Microbiology 23(6): 3009-3019 (2021), which has been published in final form at https://doi.org/10.1111/1462-2920.15504. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions Ostreococcus is a cosmopolitan marine genus of phytoplankton found in mesotrophic and oligotrophic waters, and the smallest free-living eukaryotes known to date, with a cell diameter close to 1 μm. Ostreococcus has been extensively studied as a model system to investigate viral–host dynamics in culture, yet the impact of viruses in naturally occurring populations is largely unknown. Here, we used Virus Fluorescence in situ Hybridization (VirusFISH) to visualize and quantify viral-host dynamics in natural populations of Ostreococcus during a seasonal cycle in the central Cantabrian Sea (Southern Bay of Biscay). Ostreococcus were predominantly found during summer and autumn at surface and 50 m depth, in coastal, mid-shelf and shelf waters, representing up to 21% of the picoeukaryotic communities. Viral infection was only detected in surface waters, and its impact was variable but highest from May to July and November to December, when up to half of the population was infected. Metatranscriptomic data available from the mid-shelf station unveiled that the Ostreococcus population was dominated by the species O. lucimarinus. This work represents a proof of concept that the VirusFISH technique can be used to quantify the impact of viruses on targeted populations of key microbes from complex natural communities This work was supported by the Spanish projects MEFISTO (CTM2013-43767-P, MINECO) and ALLFLAGS (CTM2016-75083-R, MINECO). YMC was supported by a FPI Spanish fellowship (BES-2014-067849), SY was supported by a Juan de la Cierva fellowship (IJCI-2017-34245) and LAS was supported by a Juan de la Cierva and Ramon y Cajal contract (RYC-2012-11404). MS was supported by a Viera y Clavijo contract funded by the ACIISI and the ULPGC. NAG was supported by Basque Government's PhD-program fellowship With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI) Peer reviewed 2021-07-14T08:39:07Z 2021-07-14T08:39:07Z 2021-06 artículo http://purl.org/coar/resource_type/c_6501 Environmental Microbiology 23(6): 3009-3019 (2021) 1462-2912 CEX2019-000928-S http://hdl.handle.net/10261/245807 10.1111/1462-2920.15504 1462-2920 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2016-75083-R Preprint https://doi.org/10.1111/1462-2920.15504 Sí open Society for Applied Microbiology |
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Special issue on Oxygen Minimum Zone Microbiology.-- 11 pages, 5 figures, 3 tables, supporting information https://doi.org/10.1111/1462-2920.15504.-- This is the pre-peer reviewed version of the following article: Yaiza M. Castillo, Irene Forn, Sheree Yau, Xosé Anxelu G. Morán, Laura Alonso-Sáez, Néstor Arandia-Gorostidi, Dolors Vaqué, Marta Sebastián; Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH; Environmental Microbiology 23(6): 3009-3019 (2021), which has been published in final form at https://doi.org/10.1111/1462-2920.15504. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Castillo, Yaiza Forn, Irene Yau, Sheree Morán, Xosé Anxelu G. Alonso-Sáez, Laura Arandia-Gorostidi, Néstor Vaqué, Dolors Sebastián, Marta |
| format |
artículo |
| author |
Castillo, Yaiza Forn, Irene Yau, Sheree Morán, Xosé Anxelu G. Alonso-Sáez, Laura Arandia-Gorostidi, Néstor Vaqué, Dolors Sebastián, Marta |
| spellingShingle |
Castillo, Yaiza Forn, Irene Yau, Sheree Morán, Xosé Anxelu G. Alonso-Sáez, Laura Arandia-Gorostidi, Néstor Vaqué, Dolors Sebastián, Marta Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH |
| author_sort |
Castillo, Yaiza |
| title |
Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH |
| title_short |
Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH |
| title_full |
Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH |
| title_fullStr |
Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH |
| title_full_unstemmed |
Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH |
| title_sort |
seasonal dynamics of natural ostreococcus viral infection at the single cell level using virusfish |
| publisher |
Society for Applied Microbiology |
| publishDate |
2021-06 |
| url |
http://hdl.handle.net/10261/245807 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003329 |
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