Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling

Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling

17 pages, 7 figures, 2 tables, additional files.-- The complete design was deposited at the Gene Expression Omnibus NCBI database GSE47400 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=exgnmkgcfbwnhkp&acc=GSE47400 and the platform that validates the microarray can be found at GPL13443.-- Erratum to: Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling, BMC Genomics 18(1): 513 (2017), https://doi.org/10.1186/s12864-017-3909-x

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Main Authors: Blázquez, Mercedes, Medina, Paula, Crespo, Berta, Gómez, Ana, Zanuy, Silvia
Other Authors: Ministerio de Ciencia e Innovación (España)
Format: artículo biblioteca
Published: BioMed Central 2017-06-05
Subjects:Spermatogenesis, Cell cycle, Meiosis, Retinoic acid, Teleosts,
Online Access:http://hdl.handle.net/10261/151172
http://dx.doi.org/10.13039/501100004837
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spelling dig-icm-es-10261-1511722021-12-27T16:09:28Z Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling Blázquez, Mercedes Medina, Paula Crespo, Berta Gómez, Ana Zanuy, Silvia Ministerio de Ciencia e Innovación (España) Universidad de Antofagasta Spermatogenesis Cell cycle Meiosis Retinoic acid Teleosts 17 pages, 7 figures, 2 tables, additional files.-- The complete design was deposited at the Gene Expression Omnibus NCBI database GSE47400 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=exgnmkgcfbwnhkp&acc=GSE47400 and the platform that validates the microarray can be found at GPL13443.-- Erratum to: Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling, BMC Genomics 18(1): 513 (2017), https://doi.org/10.1186/s12864-017-3909-x Spermatogenesis is a complex process characterized by the activation and/or repression of a number of genes in a spatio-temporal manner. Pubertal development in males starts with the onset of the first spermatogenesis and implies the division of primary spermatogonia and their subsequent entry into meiosis. This study is aimed at the characterization of genes involved in the onset of puberty in European sea bass, and constitutes the first transcriptomic approach focused on meiosis in this species. [Results] European sea bass testes collected at the onset of puberty (first successful reproduction) were grouped in stage I (resting stage), and stage II (proliferative stage). Transition from stage I to stage II was marked by an increase of 11ketotestosterone (11KT), the main fish androgen, whereas the transcriptomic study resulted in 315 genes differentially expressed between the two stages. The onset of puberty induced 1) an up-regulation of genes involved in cell proliferation, cell cycle and meiosis progression, 2) changes in genes related with reproduction and growth, and 3) a down-regulation of genes included in the retinoic acid (RA) signalling pathway. The analysis of GO-terms and biological pathways showed that cell cycle, cell division, cellular metabolic processes, and reproduction were affected, consistent with the early events that occur during the onset of puberty. Furthermore, changes in the expression of three RA nuclear receptors point at the importance of the RA-signalling pathway during this period, in agreement with its role in meiosis. [Conclusion] The results contribute to boost our knowledge of the early molecular and endocrine events that trigger pubertal development and the onset of spermatogenesis in fish. These include an increase in 11KT plasma levels and changes in the expression of several genes involved in cell proliferation, cell cycle progression, meiosis or RA-signalling pathway. Moreover, the results can be applied to study meiosis in this economically important fish species for Mediterranean countries, and may help to develop tools for its sustainable aquaculture. This work was supported by Aquagenomics project (CSD2007-0002) from the Spanish Ministry of Science and Innovation to SZ and a MICINN project (AGL2011-28890) to AG. PM was supported by a grant from the University of Antofagasta MECE2 (ANT0806) Peer reviewed 2017-06-11T03:15:46Z 2017-06-11T03:15:46Z 2017-06-05 2017-06-11T03:15:47Z artículo http://purl.org/coar/resource_type/c_6501 BMC Genomics 18(1): 441 (2017) 1471-2164 http://hdl.handle.net/10261/151172 10.1186/s12864-017-3823-2 http://dx.doi.org/10.13039/501100004837 28583077 Publisher's version https://doi.org/10.1186/s12864-017-3823-2 Sí open BioMed Central
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
topic Spermatogenesis
Cell cycle
Meiosis
Retinoic acid
Teleosts
Spermatogenesis
Cell cycle
Meiosis
Retinoic acid
Teleosts
spellingShingle Spermatogenesis
Cell cycle
Meiosis
Retinoic acid
Teleosts
Spermatogenesis
Cell cycle
Meiosis
Retinoic acid
Teleosts
Blázquez, Mercedes
Medina, Paula
Crespo, Berta
Gómez, Ana
Zanuy, Silvia
Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling
description 17 pages, 7 figures, 2 tables, additional files.-- The complete design was deposited at the Gene Expression Omnibus NCBI database GSE47400 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=exgnmkgcfbwnhkp&acc=GSE47400 and the platform that validates the microarray can be found at GPL13443.-- Erratum to: Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling, BMC Genomics 18(1): 513 (2017), https://doi.org/10.1186/s12864-017-3909-x
author2 Ministerio de Ciencia e Innovación (España)
author_facet Ministerio de Ciencia e Innovación (España)
Blázquez, Mercedes
Medina, Paula
Crespo, Berta
Gómez, Ana
Zanuy, Silvia
format artículo
topic_facet Spermatogenesis
Cell cycle
Meiosis
Retinoic acid
Teleosts
author Blázquez, Mercedes
Medina, Paula
Crespo, Berta
Gómez, Ana
Zanuy, Silvia
author_sort Blázquez, Mercedes
title Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling
title_short Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling
title_full Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling
title_fullStr Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling
title_full_unstemmed Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling
title_sort identification of conserved genes triggering puberty in european sea bass males (dicentrarchus labrax) by microarray expression profiling
publisher BioMed Central
publishDate 2017-06-05
url http://hdl.handle.net/10261/151172
http://dx.doi.org/10.13039/501100004837
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