Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)

Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)

11 pages, 4 figures, 2 tables

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Bibliographic Details
Main Authors: Galluzzi, Luca, Penna, Antonella, Bertozzini, Elena, Giacobbe, Maria Grazia, Vila, Magda, Garcés, Esther, Prioli, Silvia, Magnani, Mauro
Other Authors: European Commission
Format: artículo biblioteca
Published: Elsevier
Subjects:Alexandrium minutum, ITS, Mytilus galloprovincialis, PCR, rDNA,
Online Access:http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100005401
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spelling dig-icm-es-10261-1297352020-12-10T07:45:16Z Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis) Galluzzi, Luca Penna, Antonella Bertozzini, Elena Giacobbe, Maria Grazia Vila, Magda Garcés, Esther Prioli, Silvia Magnani, Mauro European Commission Ministero delle Politiche Agricole Alimentari e Forestali Alexandrium minutum ITS Mytilus galloprovincialis PCR rDNA 11 pages, 4 figures, 2 tables Paralytic shellfish poisoning (PSP) is a syndrome caused by the consumption of shellfish contaminated with neurotoxins produced by organisms of the marine dinoflagellate genus Alexandrium. A. minutum is the most widespread species responsible for PSP in the Western Mediterranean basin. The standard monitoring of shellfish farms for the presence of harmful algae and related toxins usually requires the microscopic examination of phytoplankton populations, bioassays and toxin determination by HPLC. These procedures are time-consuming and require remarkable experience, thus limiting the number of specimens that can be analyzed by a single laboratory unit. Molecular biology techniques may be helpful in the detection of target microorganisms in field samples. In this study, we developed a qualitative PCR assay for the rapid detection of all potentially toxic species belonging to the Alexandrium genus and specifically A. minutum, in contaminated mussels. Alexandrium genus-specific primers were designed to target the 5.8S rDNA region, while an A. minutum species-specific primer was designed to bind in the ITS1 region. The assay was validated using several fixed seawater samples from the Mediterranean basin, which were analyzed using PCR along with standard microscopy procedures. The assay provided a rapid method for monitoring the presence of Alexandrium spp. in mussel tissues, as well as in seawater samples. The results showed that PCR is a valid, rapid alternative procedure for the detection of target phytoplankton species either in seawater or directly in mussels, where microalgae can accumulate. © 2005 Elsevier B.V. All rights reserved This work was partially supported by the EU STRATEGY project contract no. EVK3-CT-2001-00046, EU CHEMAG project PPC00-3045 (2000) and Ministero delle Politiche Agricole e Forestali (MiPAF) project 6C18 Peer Reviewed 2005-11 2016-03-04T07:43:55Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.hal.2005.01.004 issn: 1568-9883 Harmful Algae 4(6): 973-983 (2005) 10261/129735 10.1016/j.hal.2005.01.004 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100005401 https://doi.org/10.1016/j.hal.2005.01.004 Sí none Elsevier
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
topic Alexandrium minutum
ITS
Mytilus galloprovincialis
PCR
rDNA
Alexandrium minutum
ITS
Mytilus galloprovincialis
PCR
rDNA
spellingShingle Alexandrium minutum
ITS
Mytilus galloprovincialis
PCR
rDNA
Alexandrium minutum
ITS
Mytilus galloprovincialis
PCR
rDNA
Galluzzi, Luca
Penna, Antonella
Bertozzini, Elena
Giacobbe, Maria Grazia
Vila, Magda
Garcés, Esther
Prioli, Silvia
Magnani, Mauro
Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)
description 11 pages, 4 figures, 2 tables
author2 European Commission
author_facet European Commission
Galluzzi, Luca
Penna, Antonella
Bertozzini, Elena
Giacobbe, Maria Grazia
Vila, Magda
Garcés, Esther
Prioli, Silvia
Magnani, Mauro
format artículo
topic_facet Alexandrium minutum
ITS
Mytilus galloprovincialis
PCR
rDNA
author Galluzzi, Luca
Penna, Antonella
Bertozzini, Elena
Giacobbe, Maria Grazia
Vila, Magda
Garcés, Esther
Prioli, Silvia
Magnani, Mauro
author_sort Galluzzi, Luca
title Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)
title_short Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)
title_full Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)
title_fullStr Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)
title_full_unstemmed Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)
title_sort development of a qualitative pcr method for the alexandrium spp. (dinophyceae) detection in contaminated mussels (mytilus galloprovincialis)
publisher Elsevier
url http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100005401
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