RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides
7 páginas, 4 figuras, 1 tabla.
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2009-12
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| Subjects: | AChE, RNA interference, Ace genes, Cockroaches, Insecticides, |
| Online Access: | http://hdl.handle.net/10261/43716 |
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dig-ibe-es-10261-437162020-10-20T12:48:31Z RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides Revuelta, Luis Piulachs, Maria-Dolors Bellés, Xavier Castañera, Pedro Ortego, Félix Díaz-Ruíz, José Ramón Hernández-Crespo, Pedro Tenllado, Francisco AChE RNA interference Ace genes Cockroaches Insecticides 7 páginas, 4 figuras, 1 tabla. Cyclorrhapha insect genomes contain a single acetylcholinesterase (AChE) gene while other insects contain at least two ace genes (ace1 and ace2). In this study we tested the hypothesis that the two ace paralogous from Blattella germanica have different contributions to AChE activity, using RNA interference (RNAi) to knockdown each one individually. Paralogous-specific depletion of Bgace transcripts was evident in ganglia of injected cockroaches, although the effects at the protein level were less pronounced. Using spectrophotometric and zymogram measurements, we obtained evidence that BgAChE1 represents 65–75% of the total AChE activity in nerve tissue demonstrating that ace1 encodes a predominant AChE. A significant increase in sensitivity of Bgace1-interfered cockroaches was observed after 48 h of exposure to chlorpyrifos. In contrast, Bgace2 knockdown had a negligible effect on mortality to this organophosphate. These results point out a key role, qualitative and/or quantitative, of AChE1 as target of organophosphate insecticides in this species. Silencing the expression of Bgace1 but not Bgace2 also produced an increased mortality in insects when synergized with lambda-cyhalothrin, a situation which resembles the synergistic effects observed between organophosphates and pyrethroids. Gene silencing of ace genes by RNAi offers an exciting approach for examining a possible functional differentiation in ace paralogous. L.R.was recipient of a predoctoral FPI fellowship from Ministerio de Ciencia e Innovación in Spain (MICINN). Thisworkwas supported by Grants BIO2003-03428, BIO2006-10944, AGL2007-63388/AGR, BFU2008-00484 and CGL2008-03517/BOS from MICINN. Peer reviewed 2011-12-26T11:46:49Z 2011-12-26T11:46:49Z 2009-12 artículo http://purl.org/coar/resource_type/c_6501 Insect Biochemistry and Molecular Biology 39(12): 913-919 (2009) 0965-1748 http://hdl.handle.net/10261/43716 10.1016/j.ibmb.2009.11.001 en http://dx.doi.org/10.1016/j.ibmb.2009.11.001 none Elsevier |
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English |
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AChE RNA interference Ace genes Cockroaches Insecticides AChE RNA interference Ace genes Cockroaches Insecticides |
| spellingShingle |
AChE RNA interference Ace genes Cockroaches Insecticides AChE RNA interference Ace genes Cockroaches Insecticides Revuelta, Luis Piulachs, Maria-Dolors Bellés, Xavier Castañera, Pedro Ortego, Félix Díaz-Ruíz, José Ramón Hernández-Crespo, Pedro Tenllado, Francisco RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides |
| description |
7 páginas, 4 figuras, 1 tabla. |
| format |
artículo |
| topic_facet |
AChE RNA interference Ace genes Cockroaches Insecticides |
| author |
Revuelta, Luis Piulachs, Maria-Dolors Bellés, Xavier Castañera, Pedro Ortego, Félix Díaz-Ruíz, José Ramón Hernández-Crespo, Pedro Tenllado, Francisco |
| author_facet |
Revuelta, Luis Piulachs, Maria-Dolors Bellés, Xavier Castañera, Pedro Ortego, Félix Díaz-Ruíz, José Ramón Hernández-Crespo, Pedro Tenllado, Francisco |
| author_sort |
Revuelta, Luis |
| title |
RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides |
| title_short |
RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides |
| title_full |
RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides |
| title_fullStr |
RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides |
| title_full_unstemmed |
RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides |
| title_sort |
rnai of ace1 and ace2 in blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides |
| publisher |
Elsevier |
| publishDate |
2009-12 |
| url |
http://hdl.handle.net/10261/43716 |
| work_keys_str_mv |
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| _version_ |
1777668539171209216 |