Molecular systematics, higher-rank classification and Gondwanan origins of Cryptocephalinae leaf beetles
With 5,300 species, the Cryptocephalinae is the fourth largest subfamily of the megadiverse Chrysomelidae beetles. This subfamily currently merges groups that were traditionally considered subfamilies of their own, including the fulcidacines, clytrines and cryptocephalines, and it is believed to be sister to the Lamprosomatinae, forming a natural group characterized by immature stages developing within the protection of a solid case of organic matter known as scatoshell. However, hypotheses on the higher-level systematics of this diverse group and the recognition of its closest relatives have been numerous and none of them tested with phylogenetic approaches, save for an unpublished study based on morphology. In this work, we provide the first molecular phylogenetic hypothesis for a large dataset of Cryptocephalinae (167 species, 66 genera) based on the analysis of five gene fragments, including up to 3,488 aligned nucleotide positions of the mitochondrial DNA cox1 and rrnS, and the nuclearly encoded LSU, elongation factor 1-alpha and polyadenylate binding protein 1. The phylogeny confirms the sister-group relationship between Cryptocephalinae and Lamprosomatinae and identifies the Eumolpinae as the sister to this assemblage. Moreover, it partly refutes the current system for Cryptocephalinae giving support to five tribes in the subfamily: Fulcidacini and Clytrini, matching the current system, Pachybrachini separated from Cryptocephalini, and a new subantarctic tribe, Mylassini trib. n., divergent from the other lineages. Among Clytrini, data support inclusion of Ischyopachina within New World Babiina, which together with New World Megalostomina would be sister to Old World Clytrina. In Cryptocephalini, after exclusion of pachybrachines, four subtribes are distinguished, of which only Stylosomina matches the current system. Otherwise, the current Monachulina are dismantled into three clades deserving subtribal status, one of which, former New World Monachulina genera, is unambiguously transferred to Cryptocephalina. A molecular clock calibrated using paleontological and biogeographic information places the origin of the subfamily in the Early Cretaceous and the split of the main evolutionary lineages temporally and geographically congruent with the fragmentation of major Gondwanan territories.
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John Wiley & Sons
2021-07-19
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Subjects: | Biogeography, Early Cretaceous, Fossil calibration, Monophyly test, Multilocus phylogeny, Suprageneric systematics, Taxonomy, |
Online Access: | http://hdl.handle.net/10261/250516 https://doi.org/10.1111/zsc.12501 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 |
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Biogeography Early Cretaceous Fossil calibration Monophyly test Multilocus phylogeny Suprageneric systematics Taxonomy Biogeography Early Cretaceous Fossil calibration Monophyly test Multilocus phylogeny Suprageneric systematics Taxonomy |
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Biogeography Early Cretaceous Fossil calibration Monophyly test Multilocus phylogeny Suprageneric systematics Taxonomy Biogeography Early Cretaceous Fossil calibration Monophyly test Multilocus phylogeny Suprageneric systematics Taxonomy Gómez-Zurita, Jesús Cardoso, Anabela Molecular systematics, higher-rank classification and Gondwanan origins of Cryptocephalinae leaf beetles |
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With 5,300 species, the Cryptocephalinae is the fourth largest subfamily of the megadiverse Chrysomelidae beetles. This subfamily currently merges groups that were traditionally considered subfamilies of their own, including the fulcidacines, clytrines and cryptocephalines, and it is believed to be sister to the Lamprosomatinae, forming a natural group characterized by immature stages developing within the protection of a solid case of organic matter known as scatoshell. However, hypotheses on the higher-level systematics of this diverse group and the recognition of its closest relatives have been numerous and none of them tested with phylogenetic approaches, save for an unpublished study based on morphology. In this work, we provide the first molecular phylogenetic hypothesis for a large dataset of Cryptocephalinae (167 species, 66 genera) based on the analysis of five gene fragments, including up to 3,488 aligned nucleotide positions of the mitochondrial DNA cox1 and rrnS, and the nuclearly encoded LSU, elongation factor 1-alpha and polyadenylate binding protein 1. The phylogeny confirms the sister-group relationship between Cryptocephalinae and Lamprosomatinae and identifies the Eumolpinae as the sister to this assemblage. Moreover, it partly refutes the current system for Cryptocephalinae giving support to five tribes in the subfamily: Fulcidacini and Clytrini, matching the current system, Pachybrachini separated from Cryptocephalini, and a new subantarctic tribe, Mylassini trib. n., divergent from the other lineages. Among Clytrini, data support inclusion of Ischyopachina within New World Babiina, which together with New World Megalostomina would be sister to Old World Clytrina. In Cryptocephalini, after exclusion of pachybrachines, four subtribes are distinguished, of which only Stylosomina matches the current system. Otherwise, the current Monachulina are dismantled into three clades deserving subtribal status, one of which, former New World Monachulina genera, is unambiguously transferred to Cryptocephalina. A molecular clock calibrated using paleontological and biogeographic information places the origin of the subfamily in the Early Cretaceous and the split of the main evolutionary lineages temporally and geographically congruent with the fragmentation of major Gondwanan territories. |
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Ministerio de Ciencia e Innovación (España) |
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Ministerio de Ciencia e Innovación (España) Gómez-Zurita, Jesús Cardoso, Anabela |
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Biogeography Early Cretaceous Fossil calibration Monophyly test Multilocus phylogeny Suprageneric systematics Taxonomy |
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Gómez-Zurita, Jesús Cardoso, Anabela |
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Gómez-Zurita, Jesús |
title |
Molecular systematics, higher-rank classification and Gondwanan origins of Cryptocephalinae leaf beetles |
title_short |
Molecular systematics, higher-rank classification and Gondwanan origins of Cryptocephalinae leaf beetles |
title_full |
Molecular systematics, higher-rank classification and Gondwanan origins of Cryptocephalinae leaf beetles |
title_fullStr |
Molecular systematics, higher-rank classification and Gondwanan origins of Cryptocephalinae leaf beetles |
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Molecular systematics, higher-rank classification and Gondwanan origins of Cryptocephalinae leaf beetles |
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molecular systematics, higher-rank classification and gondwanan origins of cryptocephalinae leaf beetles |
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John Wiley & Sons |
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2021-07-19 |
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http://hdl.handle.net/10261/250516 https://doi.org/10.1111/zsc.12501 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 |
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AT gomezzuritajesus molecularsystematicshigherrankclassificationandgondwananoriginsofcryptocephalinaeleafbeetles AT cardosoanabela molecularsystematicshigherrankclassificationandgondwananoriginsofcryptocephalinaeleafbeetles |
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dig-ibe-es-10261-2505162021-09-21T06:19:54Z Molecular systematics, higher-rank classification and Gondwanan origins of Cryptocephalinae leaf beetles Gómez-Zurita, Jesús Cardoso, Anabela Ministerio de Ciencia e Innovación (España) European Commission Biogeography Early Cretaceous Fossil calibration Monophyly test Multilocus phylogeny Suprageneric systematics Taxonomy With 5,300 species, the Cryptocephalinae is the fourth largest subfamily of the megadiverse Chrysomelidae beetles. This subfamily currently merges groups that were traditionally considered subfamilies of their own, including the fulcidacines, clytrines and cryptocephalines, and it is believed to be sister to the Lamprosomatinae, forming a natural group characterized by immature stages developing within the protection of a solid case of organic matter known as scatoshell. However, hypotheses on the higher-level systematics of this diverse group and the recognition of its closest relatives have been numerous and none of them tested with phylogenetic approaches, save for an unpublished study based on morphology. In this work, we provide the first molecular phylogenetic hypothesis for a large dataset of Cryptocephalinae (167 species, 66 genera) based on the analysis of five gene fragments, including up to 3,488 aligned nucleotide positions of the mitochondrial DNA cox1 and rrnS, and the nuclearly encoded LSU, elongation factor 1-alpha and polyadenylate binding protein 1. The phylogeny confirms the sister-group relationship between Cryptocephalinae and Lamprosomatinae and identifies the Eumolpinae as the sister to this assemblage. Moreover, it partly refutes the current system for Cryptocephalinae giving support to five tribes in the subfamily: Fulcidacini and Clytrini, matching the current system, Pachybrachini separated from Cryptocephalini, and a new subantarctic tribe, Mylassini trib. n., divergent from the other lineages. Among Clytrini, data support inclusion of Ischyopachina within New World Babiina, which together with New World Megalostomina would be sister to Old World Clytrina. In Cryptocephalini, after exclusion of pachybrachines, four subtribes are distinguished, of which only Stylosomina matches the current system. Otherwise, the current Monachulina are dismantled into three clades deserving subtribal status, one of which, former New World Monachulina genera, is unambiguously transferred to Cryptocephalina. A molecular clock calibrated using paleontological and biogeographic information places the origin of the subfamily in the Early Cretaceous and the split of the main evolutionary lineages temporally and geographically congruent with the fragmentation of major Gondwanan territories. This work was funded by the “Agencia Estatal de Investigación” of the Spanish Ministry of Science and Innovation with Grant No. CGL2017-83324-P, also with the contribution of funds from the European Regional Development Fund 1 INTRODUCTION 2 MATERIAL AND METHODS 2.1 Sampling 2.2 Phylogenetic marker selection and laboratory procedures 2.3 Sequence editing and preparation of phylogenetic matrices 2.4 Phylogenetic inference 2.5 Topology tests 2.6 Molecular clock analyses 3 RESULTS 3.1 Molecular dataset available for analysis 3.2 Phylogenetic relationships of Cryptocephalinae with other subfamilies 3.3 Molecular phylogeny of the Cryptocephalinae 3.4 Validation of monophyly hypotheses 3.5 Inferred ages of relevant clades 4 DISCUSSION 4.1 Phylogenetic support of Cryptocephalinae and their relatives 4.2 Higher-level systematics of the Cryptocephalinae 4.3 The tribes of Cryptocephalinae 4.3.1 Mylassini, a new tribe of Cryptocephalinae 4.3.2 Fulcidacini 4.3.3 Pachybrachini 4.3.4 Clytrini 4.3.5 Cryptocephalini 4.4 The evolution of the Cryptocephalinae ACKNOWLEDGEMENTS 2021-09-20T07:40:03Z 2021-09-20T07:40:03Z 2021-07-19 2021-09-20T07:40:03Z artículo http://purl.org/coar/resource_type/c_6501 doi: https://doi.org/10.1111/zsc.12501 issn: 0300-3256 e-issn: 1463-6409 Zoologica Scripta 50,5: 592- 615 (2021) http://hdl.handle.net/10261/250516 https://doi.org/10.1111/zsc.12501 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CGL2017-83324-P Publisher's version https://doi.org/10.1111/zsc.12501 Sí open John Wiley & Sons |