Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation

Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation

Trabajo presentado en la Annual Meeting of the Society for Molecular Biology and Evolution (SMBE 2015), celebrada en Viena del 12 al 16 de julio de 2015.

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Main Authors: Mandage, Rajendra, Santpere, Gabriel, Navarro, Arcadi
Format: póster de congreso biblioteca
Language:English
Published: 2015-07
Online Access:http://hdl.handle.net/10261/153631
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spelling dig-ibe-es-10261-1536312018-10-03T07:44:32Z Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation Mandage, Rajendra Santpere, Gabriel Navarro, Arcadi Trabajo presentado en la Annual Meeting of the Society for Molecular Biology and Evolution (SMBE 2015), celebrada en Viena del 12 al 16 de julio de 2015. Worldwide, >90% of the adult population is infected by the Epstein-Barr virus (EBV), which has been an evolutionary companion of our lineage for millions of years. EBV remains within the host after primary infection and can cause mononucleosis. There is also evidence linking it with multiple sclerosis and different types of tumors. Although the EBV has been the focus of extensive research work, much remain s unknown about what makes some individuals more sensitive to EBV infection and to adverse outcomes as a result of infection. The EBV is used to transform B-cells into lymphoblastoid cell lines (LCLs). We hypothesized that differences among individual LCLs in the EBV load resulting from EBV transformation may reflect different genetic susceptibility to EBV infection. To test this hypothesis, we retrieved whole-genome sequenced LCL reads 2215 samples sequenced within the 1000 Genome Project and derived from 26 different populations worldwide. We subjected these samples to in silico viral load estimation, and the accuracy was validated by RT-PCR. Our results showed considerable differences in viral load among populations, while no significant difference was observed between males and females. The proper estimation of EBV load has made it possible to perform a genome wide association analysis (GWAS) between estimated EBV load and genetic variants determined within the 1000 genome project samples. GWAS yielded many putative candidate genes that necessitate further evaluation to reveal the biological mechanisms underlying EBV load and EBV associated diseases. No 2017-07-27T10:30:50Z 2017-07-27T10:30:50Z 2015-07 póster de congreso http://purl.org/coar/resource_type/c_6670 Annual Meeting of the Society for Molecular Biology and Evolution (2015) http://hdl.handle.net/10261/153631 en Sí open
institution IBE ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ibe-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IBE España
language English
description Trabajo presentado en la Annual Meeting of the Society for Molecular Biology and Evolution (SMBE 2015), celebrada en Viena del 12 al 16 de julio de 2015.
format póster de congreso
author Mandage, Rajendra
Santpere, Gabriel
Navarro, Arcadi
spellingShingle Mandage, Rajendra
Santpere, Gabriel
Navarro, Arcadi
Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation
author_facet Mandage, Rajendra
Santpere, Gabriel
Navarro, Arcadi
author_sort Mandage, Rajendra
title Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation
title_short Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation
title_full Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation
title_fullStr Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation
title_full_unstemmed Genetic factors affecting EBV Load in Transformed LCLs from the 1000 Genome Project: a GWAS on Transformation
title_sort genetic factors affecting ebv load in transformed lcls from the 1000 genome project: a gwas on transformation
publishDate 2015-07
url http://hdl.handle.net/10261/153631
work_keys_str_mv AT mandagerajendra geneticfactorsaffectingebvloadintransformedlclsfromthe1000genomeprojectagwasontransformation
AT santperegabriel geneticfactorsaffectingebvloadintransformedlclsfromthe1000genomeprojectagwasontransformation
AT navarroarcadi geneticfactorsaffectingebvloadintransformedlclsfromthe1000genomeprojectagwasontransformation
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