Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes
6 pages, 4 tables, 1 figure.-- Available online 19 February 2010.
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2010-05
|
| Subjects: | Cereal, Breeding, HMW-GS, Pasting, GM, Wheat, |
| Online Access: | http://hdl.handle.net/10261/40744 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-iata-es-10261-40744 |
|---|---|
| record_format |
koha |
| spelling |
dig-iata-es-10261-407442020-04-07T11:34:05Z Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes León, Elena Pistón, Fernando Aouni, Racha Shewry, Peter R. Rosell, Cristina M. Martín, Antonio Barro Losada, Francisco Cereal Breeding HMW-GS Pasting GM Wheat 6 pages, 4 tables, 1 figure.-- Available online 19 February 2010. Seven transgenic lines of a commercial wheat (Triticum aestivum L.) cultivar expressing transgenic subunits 1Ax1, 1Dx5 and 1Dy10, alone or in combination have been developed. Pasting properties were determined in these transgenic lines using a Rapid Visco Analyser (RVA) in order to determine the possible impact of HMW-GS transgene expression on the starch properties. Expression of the HMW-GS transgenes increased the proportions of the corresponding 1Ax, 1Dx and 1Dy subunits affecting significantly the ratios of HMW-GS:LMW-GS and x-type:y-type HMW-GS. Starch granule size distribution varied significantly among all transgenic lines, with the Anza control and transgenic line T616 (expressing subunits 1Ax1 and 1Dy10) showing the highest and the lowest percentage of B granules, respectively. All transgenic lines increased the water binding capacities (WBC) at 25ºC and 90ºC. Line T606 (expressing subunits 1Ax1 and 1Dx5) and line T590 (expressing subunit 1Dy10) showed the lowest and the highest values for peak viscosity, respectively. Notably, lines expressing only transgenic x-type subunits (T580, T581 and T606), with high ratios of x-type:y-type HMW-GS, had low peak viscosities, final viscosities and breakdown viscosities. Line T590 had the highest breakdown viscosity while lines T606 and T581 had the lowest. Funding by the Spanish C.I.C.Y.T. (project AGL2007-65685-C02- 01). Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC) of the UK. Peer reviewed 2011-10-07T09:50:50Z 2011-10-07T09:50:50Z 2010-05 artículo http://purl.org/coar/resource_type/c_6501 Journal of Cereal Science (2010) 51: 344-349 0733-5210 http://hdl.handle.net/10261/40744 10.1016/j.jcs.2010.02.002 en http://dx.doi.org/10.1016/j.jcs.2010.02.002 open Elsevier |
| institution |
IATA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-iata-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del IATA España |
| language |
English |
| topic |
Cereal Breeding HMW-GS Pasting GM Wheat Cereal Breeding HMW-GS Pasting GM Wheat |
| spellingShingle |
Cereal Breeding HMW-GS Pasting GM Wheat Cereal Breeding HMW-GS Pasting GM Wheat León, Elena Pistón, Fernando Aouni, Racha Shewry, Peter R. Rosell, Cristina M. Martín, Antonio Barro Losada, Francisco Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes |
| description |
6 pages, 4 tables, 1 figure.-- Available online 19 February 2010. |
| format |
artículo |
| topic_facet |
Cereal Breeding HMW-GS Pasting GM Wheat |
| author |
León, Elena Pistón, Fernando Aouni, Racha Shewry, Peter R. Rosell, Cristina M. Martín, Antonio Barro Losada, Francisco |
| author_facet |
León, Elena Pistón, Fernando Aouni, Racha Shewry, Peter R. Rosell, Cristina M. Martín, Antonio Barro Losada, Francisco |
| author_sort |
León, Elena |
| title |
Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes |
| title_short |
Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes |
| title_full |
Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes |
| title_fullStr |
Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes |
| title_full_unstemmed |
Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes |
| title_sort |
pasting properties of transgenic lines of a commercial bread wheat expressing combinations of hmw glutenin subunit genes |
| publisher |
Elsevier |
| publishDate |
2010-05 |
| url |
http://hdl.handle.net/10261/40744 |
| work_keys_str_mv |
AT leonelena pastingpropertiesoftransgeniclinesofacommercialbreadwheatexpressingcombinationsofhmwgluteninsubunitgenes AT pistonfernando pastingpropertiesoftransgeniclinesofacommercialbreadwheatexpressingcombinationsofhmwgluteninsubunitgenes AT aouniracha pastingpropertiesoftransgeniclinesofacommercialbreadwheatexpressingcombinationsofhmwgluteninsubunitgenes AT shewrypeterr pastingpropertiesoftransgeniclinesofacommercialbreadwheatexpressingcombinationsofhmwgluteninsubunitgenes AT rosellcristinam pastingpropertiesoftransgeniclinesofacommercialbreadwheatexpressingcombinationsofhmwgluteninsubunitgenes AT martinantonio pastingpropertiesoftransgeniclinesofacommercialbreadwheatexpressingcombinationsofhmwgluteninsubunitgenes AT barrolosadafrancisco pastingpropertiesoftransgeniclinesofacommercialbreadwheatexpressingcombinationsofhmwgluteninsubunitgenes |
| _version_ |
1777669969650122752 |