Insight into Ultrasound-Induced Modifications of the Proteome and Flavor-Related Proteins of Unsmoked Bacon by Applying Label-Free Quantitation Technology

The aim of this study was to investigate the modifications of the proteome and flavor-related proteins in unsmoked bacon resulting from ultrasound treatment with the application of label-free quantitation technology together with bioinformatics analysis. Results showed that the expression levels of 137 proteins were markedly affected by ultrasound with most of them being significantly upregulated. The proteins distributed in the cytoplasm and the cytosol, the mitochondrion, and the nucleus were more susceptible to ultrasound treatment. Meanwhile, 20 flavor-related proteins, mostly myofibrillar proteins and metabolic enzymes mainly involved in the metabolic pathways of signaling and cellular processes and environmental information processing, were screened out. In addition, the differential expressions of flavor-related proteins induced by ultrasound were verified by western blotting. This study displayed insightful information from the proteomics perspective for a better understanding of the influential effect of ultrasound treatment on meat flavor.

Saved in:
Bibliographic Details
Main Authors: Zhang, Jian, Toldrá, Fidel, Zhang, Wangang
Other Authors: 0000-0002-9843-0193
Format: artículo biblioteca
Language:English
Published: American Chemical Society 2022-08-24
Subjects:Bioinformatics, Flavor, Label-free quantitation, Proteome, Ultrasound, Unsmoked bacon,
Online Access:http://hdl.handle.net/10261/296916
https://api.elsevier.com/content/abstract/scopus_id/85136313293
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-iata-es-10261-296916
record_format koha
spelling dig-iata-es-10261-2969162024-05-17T20:41:19Z Insight into Ultrasound-Induced Modifications of the Proteome and Flavor-Related Proteins of Unsmoked Bacon by Applying Label-Free Quantitation Technology Zhang, Jian Toldrá, Fidel Zhang, Wangang 0000-0002-9843-0193 0000-0001-6910-130X Bioinformatics Flavor Label-free quantitation Proteome Ultrasound Unsmoked bacon The aim of this study was to investigate the modifications of the proteome and flavor-related proteins in unsmoked bacon resulting from ultrasound treatment with the application of label-free quantitation technology together with bioinformatics analysis. Results showed that the expression levels of 137 proteins were markedly affected by ultrasound with most of them being significantly upregulated. The proteins distributed in the cytoplasm and the cytosol, the mitochondrion, and the nucleus were more susceptible to ultrasound treatment. Meanwhile, 20 flavor-related proteins, mostly myofibrillar proteins and metabolic enzymes mainly involved in the metabolic pathways of signaling and cellular processes and environmental information processing, were screened out. In addition, the differential expressions of flavor-related proteins induced by ultrasound were verified by western blotting. This study displayed insightful information from the proteomics perspective for a better understanding of the influential effect of ultrasound treatment on meat flavor. This work was financially supported by the China Agriculture Research System of MOF and MARA (CARS-35), the earmarked fund for the Jiangsu Agricultural Industry Technology System (JATS(2021)476), and the Kekedala City of the 4th Division Science and Technology Plan (2022GY03). Peer reviewed 2023-03-08T09:20:07Z 2023-03-08T09:20:07Z 2022-08-24 artículo http://purl.org/coar/resource_type/c_6501 Journal of Agricultural and Food Chemistry 70 (33): 10259-10270 (2022) 0021-8561 http://hdl.handle.net/10261/296916 10.1021/acs.jafc.2c03605 35947788 2-s2.0-85136313293 https://api.elsevier.com/content/abstract/scopus_id/85136313293 en Journal of agricultural and food chemistry Postprint https://doi.org/10.1021/acs.jafc.2c03605 Sí open American Chemical Society
institution IATA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-iata-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IATA España
language English
topic Bioinformatics
Flavor
Label-free quantitation
Proteome
Ultrasound
Unsmoked bacon
Bioinformatics
Flavor
Label-free quantitation
Proteome
Ultrasound
Unsmoked bacon
spellingShingle Bioinformatics
Flavor
Label-free quantitation
Proteome
Ultrasound
Unsmoked bacon
Bioinformatics
Flavor
Label-free quantitation
Proteome
Ultrasound
Unsmoked bacon
Zhang, Jian
Toldrá, Fidel
Zhang, Wangang
Insight into Ultrasound-Induced Modifications of the Proteome and Flavor-Related Proteins of Unsmoked Bacon by Applying Label-Free Quantitation Technology
description The aim of this study was to investigate the modifications of the proteome and flavor-related proteins in unsmoked bacon resulting from ultrasound treatment with the application of label-free quantitation technology together with bioinformatics analysis. Results showed that the expression levels of 137 proteins were markedly affected by ultrasound with most of them being significantly upregulated. The proteins distributed in the cytoplasm and the cytosol, the mitochondrion, and the nucleus were more susceptible to ultrasound treatment. Meanwhile, 20 flavor-related proteins, mostly myofibrillar proteins and metabolic enzymes mainly involved in the metabolic pathways of signaling and cellular processes and environmental information processing, were screened out. In addition, the differential expressions of flavor-related proteins induced by ultrasound were verified by western blotting. This study displayed insightful information from the proteomics perspective for a better understanding of the influential effect of ultrasound treatment on meat flavor.
author2 0000-0002-9843-0193
author_facet 0000-0002-9843-0193
Zhang, Jian
Toldrá, Fidel
Zhang, Wangang
format artículo
topic_facet Bioinformatics
Flavor
Label-free quantitation
Proteome
Ultrasound
Unsmoked bacon
author Zhang, Jian
Toldrá, Fidel
Zhang, Wangang
author_sort Zhang, Jian
title Insight into Ultrasound-Induced Modifications of the Proteome and Flavor-Related Proteins of Unsmoked Bacon by Applying Label-Free Quantitation Technology
title_short Insight into Ultrasound-Induced Modifications of the Proteome and Flavor-Related Proteins of Unsmoked Bacon by Applying Label-Free Quantitation Technology
title_full Insight into Ultrasound-Induced Modifications of the Proteome and Flavor-Related Proteins of Unsmoked Bacon by Applying Label-Free Quantitation Technology
title_fullStr Insight into Ultrasound-Induced Modifications of the Proteome and Flavor-Related Proteins of Unsmoked Bacon by Applying Label-Free Quantitation Technology
title_full_unstemmed Insight into Ultrasound-Induced Modifications of the Proteome and Flavor-Related Proteins of Unsmoked Bacon by Applying Label-Free Quantitation Technology
title_sort insight into ultrasound-induced modifications of the proteome and flavor-related proteins of unsmoked bacon by applying label-free quantitation technology
publisher American Chemical Society
publishDate 2022-08-24
url http://hdl.handle.net/10261/296916
https://api.elsevier.com/content/abstract/scopus_id/85136313293
work_keys_str_mv AT zhangjian insightintoultrasoundinducedmodificationsoftheproteomeandflavorrelatedproteinsofunsmokedbaconbyapplyinglabelfreequantitationtechnology
AT toldrafidel insightintoultrasoundinducedmodificationsoftheproteomeandflavorrelatedproteinsofunsmokedbaconbyapplyinglabelfreequantitationtechnology
AT zhangwangang insightintoultrasoundinducedmodificationsoftheproteomeandflavorrelatedproteinsofunsmokedbaconbyapplyinglabelfreequantitationtechnology
_version_ 1802820529530339328