SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area
Preprint disponible en http://hdl.handle.net/10261/209125
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2020-05-16
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| Subjects: | Environmental surveillance, Influent water, Reclaimed water, Concentration protocol, RNA virus, Coronavirus, |
| Online Access: | http://hdl.handle.net/10261/211707 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100011033 |
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dig-iata-es-10261-2117072022-05-16T04:30:30Z SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area Randazzo, Walter Truchado, Pilar Cuevas Ferrando, Enric Simón, Pedro Allende, Ana Sánchez Moragas, Gloria Consejo Superior de Investigaciones Científicas (España) European Commission Agencia Estatal de Investigación (España) Ministerio de Ciencia, Innovación y Universidades (España) Environmental surveillance Influent water Reclaimed water Concentration protocol RNA virus Coronavirus Preprint disponible en http://hdl.handle.net/10261/209125 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 200,000 reported COVID-19 cases in Spain resulting in more than 20,800 deaths as of April 21, 2020. Faecal shedding of SARS-CoV-2 RNA from COVID-19 patients has extensively been reported. Therefore, we investigated the occurrence of SARS-CoV-2 RNA in six wastewater treatments plants (WWTPs) serving the major municipalities within the Region of Murcia (Spain), the area with the lowest COVID-19 prevalence within Iberian Peninsula. Firstly, an aluminum hydroxide adsorption-precipitation concentration method was validated using a porcine coronavirus (Porcine Epidemic Diarrhea Virus, PEDV) and mengovirus (MgV). The procedure resulted in average recoveries of 10 ± 3.5% and 10 ± 2.1% in influent water (n = 2) and 3.3 ± 1.6% and 6.2 ± 1.0% in effluent water (n = 2) samples for PEDV and MgV, respectively. Then, the method was used to monitor the occurrence of SARS-CoV-2 from March 12 to April 14, 2020 in influent, secondary and tertiary effluent water samples. By using the real-time RT-PCR (RT-qPCR) Diagnostic Panel validated by US CDC that targets three regions of the virus nucleocapsid (N) gene, we estimated quantification of SARS-CoV-2 RNA titers in untreated wastewater waters of 5.4 ± 0.2 log10 genomic copies/L on average. Two secondary water samples resulted positive (2 out of 18) and all tertiary water samples tested as negative (0 out 12). This environmental surveillance data were compared to declared COVID-19 cases at municipality level, revealing that members of the community were shedding SARS-CoV-2 RNA in their stool even before the first cases were reported by local or national authorities in many of the cities where wastewaters have been sampled. The detection of SARS-CoV-2 in wastewater in early stages of the spread of COVID-19 highlights the relevance of this strategy as an early indicator of the infection within a specific population. At this point, this environmental surveillance could be implemented by municipalities right away as a tool, designed to help authorities to coordinate the exit strategy to gradually lift its coronavirus lockdown. The study was funded by the projects 20180705 of ESAMUR, 202070E101 of CSIC and “VIRIDIANA” AGL2017-82909 (AEI/FEDER, UE) of MICIU. WR is supported by APOSTD/2018/150 postdoctoral fellowship of Generalitat Valenciana. EC-F is recipient of a predoctoral contract from the MICINN, Call 2018. PT is holder of the RYC2018- 025510-I Ramón y Cajal contract from the MICIU. Peer reviewed 2020-05-20T09:37:34Z 2020-05-20T09:37:34Z 2020-05-16 artículo http://purl.org/coar/resource_type/c_6501 Water Research 181: 115942 (2020) 0043-1354 http://hdl.handle.net/10261/211707 10.1016/j.watres.2020.115942 1879-2448 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100011033 32425251 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-82909 AGL2017-82909/AEI/10.13039/501100011033 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC-2018- 025510-I RYC-2018-025510-I/AEI/10.13039/501100011033 Postprint https://doi.org/10.1016/j.watres.2020.115942 Sí open Elsevier |
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biblioteca |
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Environmental surveillance Influent water Reclaimed water Concentration protocol RNA virus Coronavirus Environmental surveillance Influent water Reclaimed water Concentration protocol RNA virus Coronavirus |
| spellingShingle |
Environmental surveillance Influent water Reclaimed water Concentration protocol RNA virus Coronavirus Environmental surveillance Influent water Reclaimed water Concentration protocol RNA virus Coronavirus Randazzo, Walter Truchado, Pilar Cuevas Ferrando, Enric Simón, Pedro Allende, Ana Sánchez Moragas, Gloria SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area |
| description |
Preprint disponible en http://hdl.handle.net/10261/209125 |
| author2 |
Consejo Superior de Investigaciones Científicas (España) |
| author_facet |
Consejo Superior de Investigaciones Científicas (España) Randazzo, Walter Truchado, Pilar Cuevas Ferrando, Enric Simón, Pedro Allende, Ana Sánchez Moragas, Gloria |
| format |
artículo |
| topic_facet |
Environmental surveillance Influent water Reclaimed water Concentration protocol RNA virus Coronavirus |
| author |
Randazzo, Walter Truchado, Pilar Cuevas Ferrando, Enric Simón, Pedro Allende, Ana Sánchez Moragas, Gloria |
| author_sort |
Randazzo, Walter |
| title |
SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area |
| title_short |
SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area |
| title_full |
SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area |
| title_fullStr |
SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area |
| title_full_unstemmed |
SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area |
| title_sort |
sars-cov-2 rna in wastewater anticipated covid-19 occurrence in a low prevalence area |
| publisher |
Elsevier |
| publishDate |
2020-05-16 |
| url |
http://hdl.handle.net/10261/211707 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100011033 |
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