Effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects
7 pages, 1 figure, 3 tables.
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| Format: | artículo biblioteca |
| Language: | English |
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Cambridge University Press
2009-05-18
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| Subjects: | Intestinal microbiota, Gluten-free diet, Coeliac disease, Immunity, |
| Online Access: | http://hdl.handle.net/10261/15885 |
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dig-iata-es-10261-158852019-10-23T09:46:21Z Effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects Palma, Giada de Nadal, Inmaculada Collado, María Carmen Sanz Herranz, Yolanda Intestinal microbiota Gluten-free diet Coeliac disease Immunity 7 pages, 1 figure, 3 tables. Diet influences the composition of the gut microbiota and host's health, particularly in patients suffering from food-related diseases. Coeliac disease (CD) is a permanent intolerance to cereal gluten proteins and the only therapy for the patients is to adhere to a life-long gluten-free diet (GFD). In the present preliminary study, the effects of a GFD on the composition and immune function of the gut microbiota were analysed in ten healthy subjects (mean age 30·3 years) over 1 month. Faecal microbiota was analysed by fluorescence in situ hybridisation (FISH) and quantitative PCR (qPCR). The ability of faecal bacteria to stimulate cytokine production by peripheral blood mononuclear cells (PBMC) was determined by ELISA. No significant differences in dietary intake were found before and after the GFD except for reductions (P = 0·001) in polysaccharides. Bifidobacterium, Clostridium lituseburense and Faecalibacterium prausnitzii proportions decreased (P = 0·007, P = 0·031 and P = 0·009, respectively) as a result of the GFD analysed by FISH. Bifidobacterium, Lactobacillus and Bifidobacterium longum counts decreased (P = 0·020, P = 0·001 and P = 0·017, respectively), while Enterobacteriaceae and Escherichia coli counts increased (P = 0·005 and P = 0·003) after the GFD assessed by qPCR. TNF-α, interferon-γ, IL-10 and IL-8 production by PBMC stimulated with faecal samples was also reduced (P = 0·021, P = 0·037, P = 0·002 and P = 0·007, respectively) after the diet. Therefore, the GFD led to reductions in beneficial gut bacteria populations and the ability of faecal samples to stimulate the host's immunity. Thus, the GFD may constitute an environmental variable to be considered in treated CD patients for its possible effects on gut health. This work was supported by grants AGL2008-01440/ALI and Consolider Fun-C-Food 20 CSD2007-00063 from the Spanish Ministry of Science and Innovation. Peer reviewed 2009-08-11T11:38:35Z 2009-08-11T11:38:35Z 2009-05-18 artículo http://purl.org/coar/resource_type/c_6501 British Journal of Nutrition 102(8):1154-1160 (2009) 0007-1145 http://hdl.handle.net/10261/15885 10.1017/S0007114509371767 en http://dx.doi.org/10.1017/S0007114509371767 open 193239 bytes application/pdf Cambridge University Press |
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España |
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ES |
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biblioteca |
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Europa del Sur |
| libraryname |
Biblioteca del IATA España |
| language |
English |
| topic |
Intestinal microbiota Gluten-free diet Coeliac disease Immunity Intestinal microbiota Gluten-free diet Coeliac disease Immunity |
| spellingShingle |
Intestinal microbiota Gluten-free diet Coeliac disease Immunity Intestinal microbiota Gluten-free diet Coeliac disease Immunity Palma, Giada de Nadal, Inmaculada Collado, María Carmen Sanz Herranz, Yolanda Effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects |
| description |
7 pages, 1 figure, 3 tables. |
| format |
artículo |
| topic_facet |
Intestinal microbiota Gluten-free diet Coeliac disease Immunity |
| author |
Palma, Giada de Nadal, Inmaculada Collado, María Carmen Sanz Herranz, Yolanda |
| author_facet |
Palma, Giada de Nadal, Inmaculada Collado, María Carmen Sanz Herranz, Yolanda |
| author_sort |
Palma, Giada de |
| title |
Effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects |
| title_short |
Effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects |
| title_full |
Effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects |
| title_fullStr |
Effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects |
| title_full_unstemmed |
Effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects |
| title_sort |
effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects |
| publisher |
Cambridge University Press |
| publishDate |
2009-05-18 |
| url |
http://hdl.handle.net/10261/15885 |
| work_keys_str_mv |
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