Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx

Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx

Table S2. Primer sequences used for RT-qPCR analysis.

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Bibliographic Details
Main Authors: Martins, Davide Coelho, Rubiales, Diego, Vaz Patto, María Carlota
Format: dataset biblioteca
Language:English
Published: Figshare 2022-03-24
Subjects:Genome-wide association study (GWAS), Grass pea, Partial resistance, Natural variation, Rust,
Online Access:http://hdl.handle.net/10261/330571
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spelling dig-ias-es-10261-3305712023-07-06T08:31:30Z Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx Martins, Davide Coelho Rubiales, Diego Vaz Patto, María Carlota Genome-wide association study (GWAS) Grass pea Partial resistance Natural variation Rust Table S2. Primer sequences used for RT-qPCR analysis. Uromyces pisi ([Pers.] D.C.) Wint. is an important foliar biotrophic pathogen infecting grass pea (Lathyrus sativus L.), compromising their yield stability. To date, few efforts have been made to assess the natural variation in grass pea resistance and to identify the resistance loci operating against this pathogen, limiting its efficient breeding exploitation. To overcome this knowledge gap, the genetic architecture of grass pea resistance to U. pisi was investigated using a worldwide collection of 182 accessions through a genome-wide association approach. The response of the grass pea collection to rust infection under controlled conditions and at the seedling stage did not reveal any hypersensitive response but a continuous variation for disease severity, with the identification of promising sources of partial resistance. A panel of 5,651 high-quality single-nucleotide polymorphism (SNP) markers previously generated was used to test for SNP-trait associations, based on a mixed linear model accounting for population structure. We detected seven SNP markers significantly associated with U. pisi disease severity, suggesting that partial resistance is oligogenic. Six of the associated SNP markers were located in chromosomes 4 and 6, while the remaining SNP markers had no known chromosomal position. Through comparative mapping with the pea reference genome, a total of 19 candidate genes were proposed, encoding for leucine-rich repeat, NB-ARC domain, and TGA transcription factor family, among others. Results presented in this study provided information on the availability of partial resistance in grass pea germplasm and advanced our understanding of the molecular mechanisms of quantitative resistance to rust in grass pea. Moreover, the detected associated SNP markers constitute promising genomic targets for the development of molecular tools to assist disease resistance precision breeding. Peer reviewed 2023-07-06T08:20:09Z 2023-07-06T08:20:09Z 2022-03-24 dataset Martins, Davide Coelho; Rubiales, Diego; Vaz Patto, María Carlota; 2022; Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx [Dataset]; Figshare; https://doi.org/10.3389/fpls.2022.842545.s005 http://hdl.handle.net/10261/330571 10.3389/fpls.2022.842545.s005 en Martins, Davide Coelho; Rubiales, Diego; Vaz Patto, María Carlota. Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance. https://doi.org/10.3389/fpls.2022.842545 . http://hdl.handle.net/10261/286222 https://doi.org/10.3389/fpls.2022.842545.s005 Sí open application/vnd.ms-excel Figshare
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
language English
topic Genome-wide association study (GWAS)
Grass pea
Partial resistance
Natural variation
Rust
Genome-wide association study (GWAS)
Grass pea
Partial resistance
Natural variation
Rust
spellingShingle Genome-wide association study (GWAS)
Grass pea
Partial resistance
Natural variation
Rust
Genome-wide association study (GWAS)
Grass pea
Partial resistance
Natural variation
Rust
Martins, Davide Coelho
Rubiales, Diego
Vaz Patto, María Carlota
Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
description Table S2. Primer sequences used for RT-qPCR analysis.
format dataset
topic_facet Genome-wide association study (GWAS)
Grass pea
Partial resistance
Natural variation
Rust
author Martins, Davide Coelho
Rubiales, Diego
Vaz Patto, María Carlota
author_facet Martins, Davide Coelho
Rubiales, Diego
Vaz Patto, María Carlota
author_sort Martins, Davide Coelho
title Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_short Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_full Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_fullStr Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_full_unstemmed Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_sort table_2_association mapping of lathyrus sativus disease response to uromyces pisi reveals novel loci underlying partial resistance.xlsx
publisher Figshare
publishDate 2022-03-24
url http://hdl.handle.net/10261/330571
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AT rubialesdiego table2associationmappingoflathyrussativusdiseaseresponsetouromycespisirevealsnovellociunderlyingpartialresistancexlsx
AT vazpattomariacarlota table2associationmappingoflathyrussativusdiseaseresponsetouromycespisirevealsnovellociunderlyingpartialresistancexlsx
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