Prevalence of the main viruses infecting banana in La Réunion
On Reunion Island, banana production, intended for the local market and sold through short distribution channels, represents 17% of fruit production. It contributes to the region's objectives of food self-sufficiency and public health, which rely on increased consumption of locally-produced fresh foodstuff. The damage caused by pests to banana production in Reunion is well documented, but the prevalence and impact of viral diseases remain largely unknown. Yet viruses affecting banana plants (Musa spp) are major constraints for the production of fruits and planting material, and for the conservation and exchange of Musa germplasm. Since no curative means are available against plant viruses, the control of viral diseases of banana relies mainly on prophylactic approaches such as the use of certified virusfree planting material, the replacement of infected plants by healthy ones and epidemiological surveillance, all of which require reliable, high-performance diagnostic methods that are as inexpensive as possible. We report on the first prevalence study of the main banana viruses in La Réunion. Existing immunological, immunomolecular and molecular diagnostic techniques for these viruses were successfully optimised for the analysis of large sample series, and implemented on 443 samples collected from banana plants in commercial production plots or in CIRAD's Musa genetic resources collection and from wild banana plants collected on roadsides. Our results show a low prevalence of BanMMV (2.71%), BSGFV (1.58%), BSMYV (2.26%) and BSOLV (0.45%) in the analysed samples, and constitute the first observation of BanMMV in La Réunion. They indicate that wild banana plants are potential reservoirs of BSGFV and BSMYV. Molecular analysis of BanMMV isolates from La Réunion showed that the level of molecular diversity of these isolates is significantly lower than in Guadeloupe or the rest of the world, and that there is no geographical structuring of this diversity in La Réunion. In addition, BBTV, BBrMV, BSIMV and CMV were not detected in the analysed samples.
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| Format: | thesis biblioteca |
| Language: | eng |
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Gembloux Agro-Bio Tech
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| Online Access: | http://agritrop.cirad.fr/609142/ http://agritrop.cirad.fr/609142/1/TFE_LAVENA_VANCRANENBROECK_VF.pdf |
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| Summary: | On Reunion Island, banana production, intended for the local market and sold through short distribution channels, represents 17% of fruit production. It contributes to the region's objectives of food self-sufficiency and public health, which rely on increased consumption of locally-produced fresh foodstuff. The damage caused by pests to banana production in Reunion is well documented, but the prevalence and impact of viral diseases remain largely unknown. Yet viruses affecting banana plants (Musa spp) are major constraints for the production of fruits and planting material, and for the conservation and exchange of Musa germplasm. Since no curative means are available against plant viruses, the control of viral diseases of banana relies mainly on prophylactic approaches such as the use of certified virusfree planting material, the replacement of infected plants by healthy ones and epidemiological surveillance, all of which require reliable, high-performance diagnostic methods that are as inexpensive as possible. We report on the first prevalence study of the main banana viruses in La Réunion. Existing immunological, immunomolecular and molecular diagnostic techniques for these viruses were successfully optimised for the analysis of large sample series, and implemented on 443 samples collected from banana plants in commercial production plots or in CIRAD's Musa genetic resources collection and from wild banana plants collected on roadsides. Our results show a low prevalence of BanMMV (2.71%), BSGFV (1.58%), BSMYV (2.26%) and BSOLV (0.45%) in the analysed samples, and constitute the first observation of BanMMV in La Réunion. They indicate that wild banana plants are potential reservoirs of BSGFV and BSMYV. Molecular analysis of BanMMV isolates from La Réunion showed that the level of molecular diversity of these isolates is significantly lower than in Guadeloupe or the rest of the world, and that there is no geographical structuring of this diversity in La Réunion. In addition, BBTV, BBrMV, BSIMV and CMV were not detected in the analysed samples. |
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