Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America
Geminiviruses are a group of plant-infecting viruses with single-stranded DNA genomes. Within this family, viruses in the genus Begomovirus are known to have a worldwide distribution causing a range of severe diseases in a multitude of dicotyledonous plant species. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and their ssDNA genomes can be either monopartite or bipartite. As part of a viral survey, various plants including those in the families Alliaceae, Amaranthaceae, Apiaceae, Asteraceae, Brassicaceae, Cactaceae, Cucurbitaceae, Lamiaceae, Lauraceae, Malvaceae, Oleaceae and Solanaceae were sampled and screened for begomoviruses using both a high-throughput sequencing and a begomovirus-specific primer pair approach. Based on the sequences derived using these approaches, the full-length genome of various begomoviruses were amplified from plants using abutting primers. Squash leaf curl virus (SLCV) and watermelon chlorotic stunt virus (WCSV) were identified in Cactaceae (n = 25), Solanaceae (n = 7), Cucurbitaceae (n = 2) and Lamiaceae (n = 1) samples. WCSV is an Old World bipartite begomovirus that has only recently been discovered infecting watermelons in the Americas. Our discovery of WCSV in the USA is the first indication that it has reached this country and indicates that this virus might be widespread throughout North America. Phylogenetic analysis suggests WCSV was introduced to the New World twice. The detection of begomoviruses in cactus plants suggests possible spillover events from agricultural areas into native vegetation. Since WCSV and SLCV have previously been found in mixed infections, pseudo-recombination infection experiments were conducted. We demonstrate that WCSV DNA-B is successfully trans-replicated by SLCV DNA-A despite very low degree of similarity between the replication-associated iterative sequences present in their common region, an essential feature for binding of the replication associated protein. This study highlights the importance of viral surveys for the detection of spillover events into native vegetation, but also suggests the need for more surveillance of WCSV in the USA, as this virus is a serious threat to watermelon cultivation in the Middle East.
Main Authors: | , , , , , , , , , , , , , |
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Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, virus des végétaux, begomovirus, recombinaison, Bemisia tabaci, génome, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_61d49fca, http://aims.fao.org/aos/agrovoc/c_6475, http://aims.fao.org/aos/agrovoc/c_30755, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_8114, |
Online Access: | http://agritrop.cirad.fr/598184/ http://agritrop.cirad.fr/598184/1/Fontenele_Begomovirus%20North%20America_2021b.pdf |
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H20 - Maladies des plantes virus des végétaux begomovirus recombinaison Bemisia tabaci génome http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_8114 H20 - Maladies des plantes virus des végétaux begomovirus recombinaison Bemisia tabaci génome http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_8114 |
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H20 - Maladies des plantes virus des végétaux begomovirus recombinaison Bemisia tabaci génome http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_8114 H20 - Maladies des plantes virus des végétaux begomovirus recombinaison Bemisia tabaci génome http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_8114 Fontenele, Rafaela S Bhaskara, Amulya Cobb, Ilaria N. Majure, Lucas C. Salywon, Andrew M. Avalos-Calleros, Jesús Argüello‐Astorga, Gerardo R. Schmidlin, Kara Roumagnac, Philippe Ribeiro, Simone G. Kraberger, Simona Martin, Darren Patrick Lefeuvre, Pierre Varsani, Arvind Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America |
description |
Geminiviruses are a group of plant-infecting viruses with single-stranded DNA genomes. Within this family, viruses in the genus Begomovirus are known to have a worldwide distribution causing a range of severe diseases in a multitude of dicotyledonous plant species. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and their ssDNA genomes can be either monopartite or bipartite. As part of a viral survey, various plants including those in the families Alliaceae, Amaranthaceae, Apiaceae, Asteraceae, Brassicaceae, Cactaceae, Cucurbitaceae, Lamiaceae, Lauraceae, Malvaceae, Oleaceae and Solanaceae were sampled and screened for begomoviruses using both a high-throughput sequencing and a begomovirus-specific primer pair approach. Based on the sequences derived using these approaches, the full-length genome of various begomoviruses were amplified from plants using abutting primers. Squash leaf curl virus (SLCV) and watermelon chlorotic stunt virus (WCSV) were identified in Cactaceae (n = 25), Solanaceae (n = 7), Cucurbitaceae (n = 2) and Lamiaceae (n = 1) samples. WCSV is an Old World bipartite begomovirus that has only recently been discovered infecting watermelons in the Americas. Our discovery of WCSV in the USA is the first indication that it has reached this country and indicates that this virus might be widespread throughout North America. Phylogenetic analysis suggests WCSV was introduced to the New World twice. The detection of begomoviruses in cactus plants suggests possible spillover events from agricultural areas into native vegetation. Since WCSV and SLCV have previously been found in mixed infections, pseudo-recombination infection experiments were conducted. We demonstrate that WCSV DNA-B is successfully trans-replicated by SLCV DNA-A despite very low degree of similarity between the replication-associated iterative sequences present in their common region, an essential feature for binding of the replication associated protein. This study highlights the importance of viral surveys for the detection of spillover events into native vegetation, but also suggests the need for more surveillance of WCSV in the USA, as this virus is a serious threat to watermelon cultivation in the Middle East. |
format |
article |
topic_facet |
H20 - Maladies des plantes virus des végétaux begomovirus recombinaison Bemisia tabaci génome http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_8114 |
author |
Fontenele, Rafaela S Bhaskara, Amulya Cobb, Ilaria N. Majure, Lucas C. Salywon, Andrew M. Avalos-Calleros, Jesús Argüello‐Astorga, Gerardo R. Schmidlin, Kara Roumagnac, Philippe Ribeiro, Simone G. Kraberger, Simona Martin, Darren Patrick Lefeuvre, Pierre Varsani, Arvind |
author_facet |
Fontenele, Rafaela S Bhaskara, Amulya Cobb, Ilaria N. Majure, Lucas C. Salywon, Andrew M. Avalos-Calleros, Jesús Argüello‐Astorga, Gerardo R. Schmidlin, Kara Roumagnac, Philippe Ribeiro, Simone G. Kraberger, Simona Martin, Darren Patrick Lefeuvre, Pierre Varsani, Arvind |
author_sort |
Fontenele, Rafaela S |
title |
Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America |
title_short |
Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America |
title_full |
Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America |
title_fullStr |
Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America |
title_full_unstemmed |
Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America |
title_sort |
identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in north america |
url |
http://agritrop.cirad.fr/598184/ http://agritrop.cirad.fr/598184/1/Fontenele_Begomovirus%20North%20America_2021b.pdf |
work_keys_str_mv |
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dig-cirad-fr-5981842024-01-29T03:29:57Z http://agritrop.cirad.fr/598184/ http://agritrop.cirad.fr/598184/ Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America. Fontenele Rafaela S, Bhaskara Amulya, Cobb Ilaria N., Majure Lucas C., Salywon Andrew M., Avalos-Calleros Jesús, Argüello‐Astorga Gerardo R., Schmidlin Kara, Roumagnac Philippe, Ribeiro Simone G., Kraberger Simona, Martin Darren Patrick, Lefeuvre Pierre, Varsani Arvind. 2021. Viruses, 13 (5):810, 17 p.https://doi.org/10.3390/v13050810 <https://doi.org/10.3390/v13050810> Identification of the begomoviruses squash leaf curl virus and watermelon chlorotic stunt virus in various plant samples in North America Fontenele, Rafaela S Bhaskara, Amulya Cobb, Ilaria N. Majure, Lucas C. Salywon, Andrew M. Avalos-Calleros, Jesús Argüello‐Astorga, Gerardo R. Schmidlin, Kara Roumagnac, Philippe Ribeiro, Simone G. Kraberger, Simona Martin, Darren Patrick Lefeuvre, Pierre Varsani, Arvind eng 2021 Viruses H20 - Maladies des plantes virus des végétaux begomovirus recombinaison Bemisia tabaci génome http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_3224 États-Unis d'Amérique http://aims.fao.org/aos/agrovoc/c_8114 Geminiviruses are a group of plant-infecting viruses with single-stranded DNA genomes. Within this family, viruses in the genus Begomovirus are known to have a worldwide distribution causing a range of severe diseases in a multitude of dicotyledonous plant species. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and their ssDNA genomes can be either monopartite or bipartite. As part of a viral survey, various plants including those in the families Alliaceae, Amaranthaceae, Apiaceae, Asteraceae, Brassicaceae, Cactaceae, Cucurbitaceae, Lamiaceae, Lauraceae, Malvaceae, Oleaceae and Solanaceae were sampled and screened for begomoviruses using both a high-throughput sequencing and a begomovirus-specific primer pair approach. Based on the sequences derived using these approaches, the full-length genome of various begomoviruses were amplified from plants using abutting primers. Squash leaf curl virus (SLCV) and watermelon chlorotic stunt virus (WCSV) were identified in Cactaceae (n = 25), Solanaceae (n = 7), Cucurbitaceae (n = 2) and Lamiaceae (n = 1) samples. WCSV is an Old World bipartite begomovirus that has only recently been discovered infecting watermelons in the Americas. Our discovery of WCSV in the USA is the first indication that it has reached this country and indicates that this virus might be widespread throughout North America. Phylogenetic analysis suggests WCSV was introduced to the New World twice. The detection of begomoviruses in cactus plants suggests possible spillover events from agricultural areas into native vegetation. Since WCSV and SLCV have previously been found in mixed infections, pseudo-recombination infection experiments were conducted. We demonstrate that WCSV DNA-B is successfully trans-replicated by SLCV DNA-A despite very low degree of similarity between the replication-associated iterative sequences present in their common region, an essential feature for binding of the replication associated protein. This study highlights the importance of viral surveys for the detection of spillover events into native vegetation, but also suggests the need for more surveillance of WCSV in the USA, as this virus is a serious threat to watermelon cultivation in the Middle East. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/598184/1/Fontenele_Begomovirus%20North%20America_2021b.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3390/v13050810 10.3390/v13050810 info:eu-repo/semantics/altIdentifier/doi/10.3390/v13050810 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3390/v13050810 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/?term=MW588366 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/?term=MW588417 |