Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies
Fifty-eight rhizobial strains were isolated from root nodules of Vicia faba cv. Equina and Vicia faba cv. Minor by the host-trapping method in soils collected from eleven sites in Bejaia, Eastern Algeria. Eleven genotypic groups were distinguished based on the combined PCR/RFLP of 16S rRNA, 16S–23S rRNA intergenic spacer and symbiotic (nodC and nodD-F) genes and further confirmed by multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and rpoB), the 16S rRNA gene and the nodulation genes nodC and nodD. Of the 11 genotypes, 5 were dominant and 2 were the most represented. Most of the strains shared high nodD gene sequence similarity with Rhizobium leguminosarum sv. viciae; their nodC sequences were similar to both Rhizobium leguminosarum and Rhizobium laguerreae. Sequence analyses of the 16S–23S rRNA intergenic spacer showed that all the new strains were phylogenetically related to those described from Vicia sativa and V. faba in several African, European, American and Asian countries, with which they form a group related to Rhizobium leguminosarum. Phylogenetic analysis based on MLSA of 16S rRNA, recA, atpD and rpoB genes allowed the affiliations of strain AM11R to Rhizobium leguminosarum sv. viciae and of strains EB1 and ES8 to Rhizobium laguerreae. In addition, two separate clades with <97% similarity may represent two novel genospecies within the genus Rhizobium.
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dig-cirad-fr-5883442022-02-15T07:14:35Z http://agritrop.cirad.fr/588344/ http://agritrop.cirad.fr/588344/ Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies. Belhadi Djellali, De Lajudie Philippe, Ramdani Nacer, Le Roux Christine, Boulila Farida, Tisseyre Pierre, Boulil Abdelghani, Benguedouar Amar, Kaci Yahia, Laguerre Gisèle. 2018. Systematic and Applied Microbiology, 41 (2) : 122-130.https://doi.org/10.1016/j.syapm.2017.10.004 <https://doi.org/10.1016/j.syapm.2017.10.004> Researchers Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies Belhadi, Djellali De Lajudie, Philippe Ramdani, Nacer Le Roux, Christine Boulila, Farida Tisseyre, Pierre Boulil, Abdelghani Benguedouar, Amar Kaci, Yahia Laguerre, Gisèle eng 2018 Systematic and Applied Microbiology F30 - Génétique et amélioration des plantes P34 - Biologie du sol Algérie http://aims.fao.org/aos/agrovoc/c_259 Fifty-eight rhizobial strains were isolated from root nodules of Vicia faba cv. Equina and Vicia faba cv. Minor by the host-trapping method in soils collected from eleven sites in Bejaia, Eastern Algeria. Eleven genotypic groups were distinguished based on the combined PCR/RFLP of 16S rRNA, 16S–23S rRNA intergenic spacer and symbiotic (nodC and nodD-F) genes and further confirmed by multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and rpoB), the 16S rRNA gene and the nodulation genes nodC and nodD. Of the 11 genotypes, 5 were dominant and 2 were the most represented. Most of the strains shared high nodD gene sequence similarity with Rhizobium leguminosarum sv. viciae; their nodC sequences were similar to both Rhizobium leguminosarum and Rhizobium laguerreae. Sequence analyses of the 16S–23S rRNA intergenic spacer showed that all the new strains were phylogenetically related to those described from Vicia sativa and V. faba in several African, European, American and Asian countries, with which they form a group related to Rhizobium leguminosarum. Phylogenetic analysis based on MLSA of 16S rRNA, recA, atpD and rpoB genes allowed the affiliations of strain AM11R to Rhizobium leguminosarum sv. viciae and of strains EB1 and ES8 to Rhizobium laguerreae. In addition, two separate clades with <97% similarity may represent two novel genospecies within the genus Rhizobium. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/588344/1/1-s2.0-S0723202017301741-main.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.syapm.2017.10.004 10.1016/j.syapm.2017.10.004 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.syapm.2017.10.004 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.syapm.2017.10.004 |
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F30 - Génétique et amélioration des plantes P34 - Biologie du sol http://aims.fao.org/aos/agrovoc/c_259 F30 - Génétique et amélioration des plantes P34 - Biologie du sol http://aims.fao.org/aos/agrovoc/c_259 |
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F30 - Génétique et amélioration des plantes P34 - Biologie du sol http://aims.fao.org/aos/agrovoc/c_259 F30 - Génétique et amélioration des plantes P34 - Biologie du sol http://aims.fao.org/aos/agrovoc/c_259 Belhadi, Djellali De Lajudie, Philippe Ramdani, Nacer Le Roux, Christine Boulila, Farida Tisseyre, Pierre Boulil, Abdelghani Benguedouar, Amar Kaci, Yahia Laguerre, Gisèle Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies |
description |
Fifty-eight rhizobial strains were isolated from root nodules of Vicia faba cv. Equina and Vicia faba cv. Minor by the host-trapping method in soils collected from eleven sites in Bejaia, Eastern Algeria. Eleven genotypic groups were distinguished based on the combined PCR/RFLP of 16S rRNA, 16S–23S rRNA intergenic spacer and symbiotic (nodC and nodD-F) genes and further confirmed by multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and rpoB), the 16S rRNA gene and the nodulation genes nodC and nodD. Of the 11 genotypes, 5 were dominant and 2 were the most represented. Most of the strains shared high nodD gene sequence similarity with Rhizobium leguminosarum sv. viciae; their nodC sequences were similar to both Rhizobium leguminosarum and Rhizobium laguerreae. Sequence analyses of the 16S–23S rRNA intergenic spacer showed that all the new strains were phylogenetically related to those described from Vicia sativa and V. faba in several African, European, American and Asian countries, with which they form a group related to Rhizobium leguminosarum. Phylogenetic analysis based on MLSA of 16S rRNA, recA, atpD and rpoB genes allowed the affiliations of strain AM11R to Rhizobium leguminosarum sv. viciae and of strains EB1 and ES8 to Rhizobium laguerreae. In addition, two separate clades with <97% similarity may represent two novel genospecies within the genus Rhizobium. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes P34 - Biologie du sol http://aims.fao.org/aos/agrovoc/c_259 |
author |
Belhadi, Djellali De Lajudie, Philippe Ramdani, Nacer Le Roux, Christine Boulila, Farida Tisseyre, Pierre Boulil, Abdelghani Benguedouar, Amar Kaci, Yahia Laguerre, Gisèle |
author_facet |
Belhadi, Djellali De Lajudie, Philippe Ramdani, Nacer Le Roux, Christine Boulila, Farida Tisseyre, Pierre Boulil, Abdelghani Benguedouar, Amar Kaci, Yahia Laguerre, Gisèle |
author_sort |
Belhadi, Djellali |
title |
Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies |
title_short |
Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies |
title_full |
Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies |
title_fullStr |
Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies |
title_full_unstemmed |
Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies |
title_sort |
vicia faba l. in the bejaia region of algeria is nodulated by rhizobium leguminosarum sv. viciae , rhizobium laguerreae and two new genospecies |
url |
http://agritrop.cirad.fr/588344/ http://agritrop.cirad.fr/588344/1/1-s2.0-S0723202017301741-main.pdf |
work_keys_str_mv |
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