Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090]
Amblyomma variegatum ticks are considered as main pest of ruminants in Africa and in the Caribbean, transmitting heartwater and reactivating bovine dermatophilosis. The objective of our study was to understand the role of Amblyomma saliva in tick-host interactions using proteomics and cellular biology approaches. Among the 336 proteins identified in A. variegatum saliva, a bioinformatics analysis highlighted bioactive molecules with anti-inflammatory and immuno-modulatory properties. We also characterised an ubiquitination complex that could participate in saliva-induced immune suppression of the host. We focused on the immuno-modulating effects of A. variegatum saliva on bovine peripheral blood mononuclear cells (PBMCs) and on macrophages in vitro. We first evidenced a significant decrease of lymphoproliferation for ConA stimulated PBMCs indicative of saliva immuno-suppressive properties. Then, we studied the effect of saliva on bovine macrophages, by measuring nitric oxide production and pro- or anti-inflammatory cytokines such as interleukins IL-10, IL-12p40, and TNF-α. We also analyzed activation and co-stimulatory molecules such as MHC-II, CD40, CD80 or CD86. Finally, we suggest an integrated model of interaction between tick saliva and host immune cells, with a potential effect during tick feeding to favour pathogens dissemination by decreasing host immune response efficacy. Our results bring new insights for a better understanding of tick-ruminant interactions at the molecular level, and pave the way towards integrative strategies to interfere with both the immunosuppressive and infectious processes in corresponding tick-borne diseases. (Texte intégral)
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dig-cirad-fr-5865202019-09-23T09:13:58Z http://agritrop.cirad.fr/586520/ http://agritrop.cirad.fr/586520/ Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090]. Holzmuller Philippe, Rodrigues Valérie, Fernandez Bernard, Chamayou Léo, Vercoutere Arthur, Andersen Alexandre, Demettre Edith, Seveno Martial, Vigy Oana, Aprelon Rosalie, Giraud-Girard Ken, Stachurski Frédéric, Vachiéry Nathalie. 2017. In : Book of abstracts of 9th International conference on tick and tick borne pathogens: One health. TTP9-APRC1. Cairns : TTP9-APRC1, Résumé, 49. 9th International conference on tick and tick borne pathogens, Cairns, Australie, 27 Août 2017/1 Septembre 2017.http://www.cvent.com/events/one-health-9th-tick-and-tick-borne-pathogen-conference-and-1st-asia-pacific-rickettsia-conference/custom-17-6727cb512f6d4620bfa742a79a711dd6.aspx <http://www.cvent.com/events/one-health-9th-tick-and-tick-borne-pathogen-conference-and-1st-asia-pacific-rickettsia-conference/custom-17-6727cb512f6d4620bfa742a79a711dd6.aspx> Researchers Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090] Holzmuller, Philippe Rodrigues, Valérie Fernandez, Bernard Chamayou, Léo Vercoutere, Arthur Andersen, Alexandre Demettre, Edith Seveno, Martial Vigy, Oana Aprelon, Rosalie Giraud-Girard, Ken Stachurski, Frédéric Vachiéry, Nathalie eng 2017 TTP9-APRC1 Book of abstracts of 9th International conference on tick and tick borne pathogens: One health L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Amblyomma variegatum ticks are considered as main pest of ruminants in Africa and in the Caribbean, transmitting heartwater and reactivating bovine dermatophilosis. The objective of our study was to understand the role of Amblyomma saliva in tick-host interactions using proteomics and cellular biology approaches. Among the 336 proteins identified in A. variegatum saliva, a bioinformatics analysis highlighted bioactive molecules with anti-inflammatory and immuno-modulatory properties. We also characterised an ubiquitination complex that could participate in saliva-induced immune suppression of the host. We focused on the immuno-modulating effects of A. variegatum saliva on bovine peripheral blood mononuclear cells (PBMCs) and on macrophages in vitro. We first evidenced a significant decrease of lymphoproliferation for ConA stimulated PBMCs indicative of saliva immuno-suppressive properties. Then, we studied the effect of saliva on bovine macrophages, by measuring nitric oxide production and pro- or anti-inflammatory cytokines such as interleukins IL-10, IL-12p40, and TNF-α. We also analyzed activation and co-stimulatory molecules such as MHC-II, CD40, CD80 or CD86. Finally, we suggest an integrated model of interaction between tick saliva and host immune cells, with a potential effect during tick feeding to favour pathogens dissemination by decreasing host immune response efficacy. Our results bring new insights for a better understanding of tick-ruminant interactions at the molecular level, and pave the way towards integrative strategies to interfere with both the immunosuppressive and infectious processes in corresponding tick-borne diseases. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/586520/7/ID586520.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://www.cvent.com/events/one-health-9th-tick-and-tick-borne-pathogen-conference-and-1st-asia-pacific-rickettsia-conference/custom-17-6727cb512f6d4620bfa742a79a711dd6.aspx info:eu-repo/semantics/altIdentifier/purl/http://www.cvent.com/events/one-health-9th-tick-and-tick-borne-pathogen-conference-and-1st-asia-pacific-rickettsia-conference/custom-17-6727cb512f6d4620bfa742a79a711dd6.aspx info:eu-repo/semantics/reference/purl/http://www.ttp9-aprc1.com/ |
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L72 - Organismes nuisibles des animaux L73 - Maladies des animaux L72 - Organismes nuisibles des animaux L73 - Maladies des animaux |
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L72 - Organismes nuisibles des animaux L73 - Maladies des animaux L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Holzmuller, Philippe Rodrigues, Valérie Fernandez, Bernard Chamayou, Léo Vercoutere, Arthur Andersen, Alexandre Demettre, Edith Seveno, Martial Vigy, Oana Aprelon, Rosalie Giraud-Girard, Ken Stachurski, Frédéric Vachiéry, Nathalie Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090] |
description |
Amblyomma variegatum ticks are considered as main pest of ruminants in Africa and in the Caribbean, transmitting heartwater and reactivating bovine dermatophilosis. The objective of our study was to understand the role of Amblyomma saliva in tick-host interactions using proteomics and cellular biology approaches. Among the 336 proteins identified in A. variegatum saliva, a bioinformatics analysis highlighted bioactive molecules with anti-inflammatory and immuno-modulatory properties. We also characterised an ubiquitination complex that could participate in saliva-induced immune suppression of the host. We focused on the immuno-modulating effects of A. variegatum saliva on bovine peripheral blood mononuclear cells (PBMCs) and on macrophages in vitro. We first evidenced a significant decrease of lymphoproliferation for ConA stimulated PBMCs indicative of saliva immuno-suppressive properties. Then, we studied the effect of saliva on bovine macrophages, by measuring nitric oxide production and pro- or anti-inflammatory cytokines such as interleukins IL-10, IL-12p40, and TNF-α. We also analyzed activation and co-stimulatory molecules such as MHC-II, CD40, CD80 or CD86. Finally, we suggest an integrated model of interaction between tick saliva and host immune cells, with a potential effect during tick feeding to favour pathogens dissemination by decreasing host immune response efficacy. Our results bring new insights for a better understanding of tick-ruminant interactions at the molecular level, and pave the way towards integrative strategies to interfere with both the immunosuppressive and infectious processes in corresponding tick-borne diseases. (Texte intégral) |
format |
conference_item |
topic_facet |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux |
author |
Holzmuller, Philippe Rodrigues, Valérie Fernandez, Bernard Chamayou, Léo Vercoutere, Arthur Andersen, Alexandre Demettre, Edith Seveno, Martial Vigy, Oana Aprelon, Rosalie Giraud-Girard, Ken Stachurski, Frédéric Vachiéry, Nathalie |
author_facet |
Holzmuller, Philippe Rodrigues, Valérie Fernandez, Bernard Chamayou, Léo Vercoutere, Arthur Andersen, Alexandre Demettre, Edith Seveno, Martial Vigy, Oana Aprelon, Rosalie Giraud-Girard, Ken Stachurski, Frédéric Vachiéry, Nathalie |
author_sort |
Holzmuller, Philippe |
title |
Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090] |
title_short |
Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090] |
title_full |
Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090] |
title_fullStr |
Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090] |
title_full_unstemmed |
Amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090] |
title_sort |
amblyomma variegatum saliva effects on host immune responses studied by integrative proteomics and cellular biology. [0090] |
publisher |
TTP9-APRC1 |
url |
http://agritrop.cirad.fr/586520/ http://agritrop.cirad.fr/586520/7/ID586520.pdf |
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