Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development
Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30–240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways.
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dig-cirad-fr-5827162024-01-28T23:55:06Z http://agritrop.cirad.fr/582716/ http://agritrop.cirad.fr/582716/ Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Ivamoto Suzana Tiemi, Sakuray Leonardo M., Ferreira Lucia Pires, Kitzberger Cíntia S.G., Scholz Maria Brigida dos Santos, Pot David, Leroy Thierry, Vieira Luiz Gonzaga Esteves, Domingues Douglas S., Pereira Luiz Filipe P.. 2017. Plant Physiology and Biochemistry, 111 : 340-347.https://doi.org/10.1016/j.plaphy.2016.12.004 <https://doi.org/10.1016/j.plaphy.2016.12.004> Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development Ivamoto, Suzana Tiemi Sakuray, Leonardo M. Ferreira, Lucia Pires Kitzberger, Cíntia S.G. Scholz, Maria Brigida dos Santos Pot, David Leroy, Thierry Vieira, Luiz Gonzaga Esteves Domingues, Douglas S. Pereira, Luiz Filipe P. eng 2017 Plant Physiology and Biochemistry F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires U30 - Méthodes de recherche Coffea arabica composition chimique tissu végétal diterpénoïde expression des gènes biosynthèse développement biologique PCR HPLC racine feuille fleur http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_27990 http://aims.fao.org/aos/agrovoc/c_32428 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_921 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_6651 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_2993 Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30–240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/582716/3/1-s2.0-S0981942816304624-main.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.plaphy.2016.12.004 10.1016/j.plaphy.2016.12.004 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2016.12.004 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.plaphy.2016.12.004 |
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Biblioteca del CIRAD Francia |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires U30 - Méthodes de recherche Coffea arabica composition chimique tissu végétal diterpénoïde expression des gènes biosynthèse développement biologique PCR HPLC racine feuille fleur http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_27990 http://aims.fao.org/aos/agrovoc/c_32428 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_921 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_6651 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_2993 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires U30 - Méthodes de recherche Coffea arabica composition chimique tissu végétal diterpénoïde expression des gènes biosynthèse développement biologique PCR HPLC racine feuille fleur http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_27990 http://aims.fao.org/aos/agrovoc/c_32428 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_921 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_6651 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_2993 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires U30 - Méthodes de recherche Coffea arabica composition chimique tissu végétal diterpénoïde expression des gènes biosynthèse développement biologique PCR HPLC racine feuille fleur http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_27990 http://aims.fao.org/aos/agrovoc/c_32428 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_921 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_6651 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_2993 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires U30 - Méthodes de recherche Coffea arabica composition chimique tissu végétal diterpénoïde expression des gènes biosynthèse développement biologique PCR HPLC racine feuille fleur http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_27990 http://aims.fao.org/aos/agrovoc/c_32428 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_921 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_6651 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_2993 Ivamoto, Suzana Tiemi Sakuray, Leonardo M. Ferreira, Lucia Pires Kitzberger, Cíntia S.G. Scholz, Maria Brigida dos Santos Pot, David Leroy, Thierry Vieira, Luiz Gonzaga Esteves Domingues, Douglas S. Pereira, Luiz Filipe P. Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development |
description |
Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30–240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires U30 - Méthodes de recherche Coffea arabica composition chimique tissu végétal diterpénoïde expression des gènes biosynthèse développement biologique PCR HPLC racine feuille fleur http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_27990 http://aims.fao.org/aos/agrovoc/c_32428 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_921 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_6651 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_2993 |
author |
Ivamoto, Suzana Tiemi Sakuray, Leonardo M. Ferreira, Lucia Pires Kitzberger, Cíntia S.G. Scholz, Maria Brigida dos Santos Pot, David Leroy, Thierry Vieira, Luiz Gonzaga Esteves Domingues, Douglas S. Pereira, Luiz Filipe P. |
author_facet |
Ivamoto, Suzana Tiemi Sakuray, Leonardo M. Ferreira, Lucia Pires Kitzberger, Cíntia S.G. Scholz, Maria Brigida dos Santos Pot, David Leroy, Thierry Vieira, Luiz Gonzaga Esteves Domingues, Douglas S. Pereira, Luiz Filipe P. |
author_sort |
Ivamoto, Suzana Tiemi |
title |
Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development |
title_short |
Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development |
title_full |
Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development |
title_fullStr |
Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development |
title_full_unstemmed |
Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development |
title_sort |
diterpenes biochemical profile and transcriptional analysis of cytochrome p450s genes in leaves, roots, flowers, and during coffea arabica l. fruit development |
url |
http://agritrop.cirad.fr/582716/ http://agritrop.cirad.fr/582716/3/1-s2.0-S0981942816304624-main.pdf |
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