Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants
Different transgenic tobacco lines down-regulated for either one or two enzymes of the monolignol pathway were compared for their lignin content and composition, and developmental patterns. The comparison concerned CCR and CAD down-regulated lines (homozygous or heterozygous for the transgene) and the hybrids resulting from the crossing of transgenic lines individually altered for CCR or CAD activities. Surprisingly, the crosses containing only one allele of each antisense transgene, exhibit a dramatic reduction of lignin content similar to the CCR down-regulated parent but, in contrast to this transgenic line, display a normal phenotype and only slight alterations of the shape of the vessels. Qualitatively the lignin of the double transformant displays characteristics more like the wild type control than either of the other transgenics. In the transgenics with a low lignin content, the transformations induced other biochemical changes involving polysaccharides, phenolic components of the cell wall and also soluble phenolics. These results show that the ectopic expression of a specific transgene may have a different impact depending on the genetic background and suggest that the two transgenes present in the crosses may operate synergistically to reduce the lignin content. In addition, these data confirm that plants with a severe reduction in lignin content may undergo normal development at least in controlled conditions.
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Subjects: | F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale, F62 - Physiologie végétale - Croissance et développement, |
Online Access: | http://agritrop.cirad.fr/578393/ http://agritrop.cirad.fr/578393/1/Chabannes%20et%20al.%2C%202001a.pdf |
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dig-cirad-fr-5783932022-04-22T13:09:04Z http://agritrop.cirad.fr/578393/ http://agritrop.cirad.fr/578393/ Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants. Chabannes Matthieu, Barakate Abdellah, Lapierre Catherine, Marita Jane M., Ralph John, Péan Michel, Danoun Saïda, Halpin Claire, Grima Pettenati Jacqueline, Boudet Alain-Michel. 2001. Plant Journal, 28 (3) : 257-270.https://doi.org/10.1046/j.1365-313X.2001.01140.x <https://doi.org/10.1046/j.1365-313X.2001.01140.x> Researchers Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants Chabannes, Matthieu Barakate, Abdellah Lapierre, Catherine Marita, Jane M. Ralph, John Péan, Michel Danoun, Saïda Halpin, Claire Grima Pettenati, Jacqueline Boudet, Alain-Michel eng 2001 Plant Journal F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F62 - Physiologie végétale - Croissance et développement Different transgenic tobacco lines down-regulated for either one or two enzymes of the monolignol pathway were compared for their lignin content and composition, and developmental patterns. The comparison concerned CCR and CAD down-regulated lines (homozygous or heterozygous for the transgene) and the hybrids resulting from the crossing of transgenic lines individually altered for CCR or CAD activities. Surprisingly, the crosses containing only one allele of each antisense transgene, exhibit a dramatic reduction of lignin content similar to the CCR down-regulated parent but, in contrast to this transgenic line, display a normal phenotype and only slight alterations of the shape of the vessels. Qualitatively the lignin of the double transformant displays characteristics more like the wild type control than either of the other transgenics. In the transgenics with a low lignin content, the transformations induced other biochemical changes involving polysaccharides, phenolic components of the cell wall and also soluble phenolics. These results show that the ectopic expression of a specific transgene may have a different impact depending on the genetic background and suggest that the two transgenes present in the crosses may operate synergistically to reduce the lignin content. In addition, these data confirm that plants with a severe reduction in lignin content may undergo normal development at least in controlled conditions. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/578393/1/Chabannes%20et%20al.%2C%202001a.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1046/j.1365-313X.2001.01140.x 10.1046/j.1365-313X.2001.01140.x info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1365-313X.2001.01140.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1046/j.1365-313X.2001.01140.x |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F62 - Physiologie végétale - Croissance et développement |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F62 - Physiologie végétale - Croissance et développement Chabannes, Matthieu Barakate, Abdellah Lapierre, Catherine Marita, Jane M. Ralph, John Péan, Michel Danoun, Saïda Halpin, Claire Grima Pettenati, Jacqueline Boudet, Alain-Michel Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants |
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Different transgenic tobacco lines down-regulated for either one or two enzymes of the monolignol pathway were compared for their lignin content and composition, and developmental patterns. The comparison concerned CCR and CAD down-regulated lines (homozygous or heterozygous for the transgene) and the hybrids resulting from the crossing of transgenic lines individually altered for CCR or CAD activities. Surprisingly, the crosses containing only one allele of each antisense transgene, exhibit a dramatic reduction of lignin content similar to the CCR down-regulated parent but, in contrast to this transgenic line, display a normal phenotype and only slight alterations of the shape of the vessels. Qualitatively the lignin of the double transformant displays characteristics more like the wild type control than either of the other transgenics. In the transgenics with a low lignin content, the transformations induced other biochemical changes involving polysaccharides, phenolic components of the cell wall and also soluble phenolics. These results show that the ectopic expression of a specific transgene may have a different impact depending on the genetic background and suggest that the two transgenes present in the crosses may operate synergistically to reduce the lignin content. In addition, these data confirm that plants with a severe reduction in lignin content may undergo normal development at least in controlled conditions. |
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article |
topic_facet |
F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F62 - Physiologie végétale - Croissance et développement |
author |
Chabannes, Matthieu Barakate, Abdellah Lapierre, Catherine Marita, Jane M. Ralph, John Péan, Michel Danoun, Saïda Halpin, Claire Grima Pettenati, Jacqueline Boudet, Alain-Michel |
author_facet |
Chabannes, Matthieu Barakate, Abdellah Lapierre, Catherine Marita, Jane M. Ralph, John Péan, Michel Danoun, Saïda Halpin, Claire Grima Pettenati, Jacqueline Boudet, Alain-Michel |
author_sort |
Chabannes, Matthieu |
title |
Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants |
title_short |
Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants |
title_full |
Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants |
title_fullStr |
Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants |
title_full_unstemmed |
Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants |
title_sort |
strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl coa reductase (ccr) and cinnamyl alcohol dehydrogenase (cad) in tobacco plants |
url |
http://agritrop.cirad.fr/578393/ http://agritrop.cirad.fr/578393/1/Chabannes%20et%20al.%2C%202001a.pdf |
work_keys_str_mv |
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